Laparoscopic visualization with histologic confirmation represents the best available option to date in the diagnosis of endometriosis

Marchino, Gian Luigi; Gennarelli, Gianluca; Enria, Raffaella; Bongioanni, Francesca; Lipari, Giovanni; Massobrio, M

doi:10.1016/j.fertnstert.2005.03.025

Cited by 6 publications

(5 citation statements)

References 3 publications

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“…Followed laparoscopic biopsy lesions have been used to get to know and look for lesions and perform staging which provides accuracy of the results of histopathology of 50-67%. 7,8 Although laparoscopy is regarded as a diagnostic method commonly used up to now, but this way is not always easy to do and not always accepted by the female suspect suffered from endometriosis since it is an invasive action. As a result, there is often a delay in diagnosis, and even let loose detection of disease.…”

Section: Introductionmentioning

confidence: 99%

“…1,6,9 Investigations such as ultrasound, computed tomography (CT) scan, MRI, and CA-125 is a diagnostic method that is noninvasive, but these methods only useful for diagnosing severe abnormalities. 1,[6][7][8][9] Aetiopathogenesis has been proposed for a long time, but the theory of implantation of Sampson remains the main theory used to underlie the pathogenesis concept at this time. Immunological factors, genetic, hormonal, and inflammation have also extensively covered the incidence of the disease through the theory.…”

Section: Introductionmentioning

confidence: 99%

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Simple Immunocytochemistry from Menstrual Blood in Diagnosis of Endometriosis

Madjid¹,

Hernowo²

2019

Journal of South Asian Federation of Obstetrics and Gynaecology

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Introduction:The menstrual reflux containing viable endometrial cells precede the development of endometriosis lesion. Based on that, we can assume that we can detect the presence of endometriosis by utilizing the endometrial cells in the menstrual blood sloughing. The purpose of this study was to prove that simple immunocytochemistry technique on a sample of menstrual blood may show endometrial cells. Materials and methods: A case-control study involving 60 women was performed, from February 2007 to February 2008. Screening for suspected endometriosis was performed by history taking, physical diagnosis and additional examination. Diagnostic laparoscopy or laparotomy and biopsy were performed afterward. Endometriosis histopathologically defined as the presence of epithelial cells of endometrial glands and stroma in the tissue being examined. Menstrual blood sampling is performed by taking 20 drops of blood into 20 mL preservative solution. In this study, the cells/tissues staining by immunohistochemistry using diaminobenzene (DAB), and for comparison hematoxylin-eosin staining (HE) was used so that the stromal cells which express caspase-3, caspase-9 and MMP-9 are stained brown with a blue background. Results: The endometrial cells were successfully isolated using a preservative solution, and all samples from endometriosis subjects could be analyzed for the expression of caspase-3, caspase-9, and MMP-9. Discussion: The results of this study lead to a conclusion that immunocytochemistry analysis of the menstrual blood endometrial cells can be applied as a noninvasive method for establishing the diagnosis of endometriosis in daily practice.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simple Immunocytochemistry from Menstrual Blood in Diagnosis of Endometriosis

Madjid¹,

Hernowo²

2019

Journal of South Asian Federation of Obstetrics and Gynaecology

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“…Therefore, the diagnosis of endometriosis is usually confirmed by laparoscopy, preferably performed in conjunction with histological evaluation of excised lesions. 5,7,10 The need of an invasive procedure performed by an experienced surgical team and the reported lack of consistency between laparoscopic and histological diagnosis of endometriosis, in particular for minimal/mild stages, 7,10,11 have prompted countless studies in the search of noninvasive diagnostic methods with adequate accuracy. The ideal method should noninvasively detect all types of endometriotic lesions and also provide functional information.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the diagnosis of endometriosis is usually confirmed by laparoscopy, preferably performed in conjunction with histological evaluation of excised lesions. 5,7,10…”

Section: Introductionmentioning

confidence: 99%

18F-Fluorocholine Uptake and Positron Emission Tomography Imaging in Rat Peritoneal Endometriosis

et al. 2018

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Endometriosis is a debilitating disease that still needs surgery to be confirmed. Endometriosis is associated with increased plasma levels of phosphatidylcholines. F-fluorocholine ([F]FCH) is a radiopharmaceutical that is metabolized to phosphatidylcholine inside the cells and can be traced by positron emission tomography (PET). Here we evaluate [F]FCH as a potential tool for the noninvasive diagnosis of peritoneal endometriosis. Adult female Wistar rats had autologous uterine fragments dissected and grafted to the peritoneal wall to model peritoneal endometriosis. Ex vivo biodistribution assay and PET imaging studies were performed 30 minutes after [F]FCH administration. The [F]FCH uptake was 3-fold higher in endometriotic implant tissues than in muscle or peritoneum. Positron emission tomography imaging revealed the grafted uterine tissue in contrast to surrounding structures. Region-of-interest analysis of the reconstructed images showed higher accumulation of [F]FCH by endometriotic lesions, 0.34 (0.04)% of injected dose per gram of tissue (ID/g), in comparison with muscle tissue, 0.08 (0.01)% ID/g. However, sham implants with fat tissue were also detectable in PET imaging. These preliminary findings of [F]FCH uptake by ectopic uterine tissue implants and their localization by PET imaging encourage the future evaluation of this technique to detect small superficial endometriosis lesions in humans. Study protocols need to be further perfected and adapted for tests in women with endometriosis.

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“…Dyspareunia, pain during menstruation and infertility are other symptoms of the disease. Laparoscopy and biopsy are the primary methods for histological diagnosis . Endometriosis is considered a multifactorial disease and most probably a chronic inflammation in the peritoneal cavity triggers the disease .…”

Section: Introductionmentioning

confidence: 99%