Laponite–Gelatin Nanofibrous Microsphere Promoting Human Dental Follicle Stem Cells Attachment and Osteogenic Differentiation for Noninvasive Stem Cell Transplantation

Abstract

Nanofibrous microspheres (NFM) are emerging as prominent next‐generation biomimetic injectable scaffold system for stem cell delivery and different tissue regeneration where nanofibrous topography facilitates ECM‐like stem cells niches. Addition of osteogenic bioactive nanosilicate platelets within NFM can provide osteoconductive cues to facilitate matrix mediated osteogenic differentiation of stem cells and enhance the efficiency of bone tissue regeneration. In this study, gelatin nanofibrous microspheres are prepared containing fluoride‐doped laponite XL21 (LP) using the emulsion mediated thermal induce phase separation (TIPS) technique. Systematic studies are performed to understand the effect of physicochemical properties of biomimicking NFM alone and with different concentrations of LP on human dental follicle stem cells (hDFSCs), their cellular attachment, proliferation, and osteogenic differentiation. The study highlights the effect of LP nanosilicate with biomimicking nanofibrous injectable scaffold system aiding in enhancing stem cell differentiation under normal physiological conditions compared to NFM without LP. The laponite–NFM shows suitability as excellent injectable biomaterials system for stem cell attachment, proliferation and osteogenic differentiation for stem cell transplantation and bone tissue regeneration.

Bone Regeneration Facilitated by Autologous Bioscaffold Material: Liquid Phase of Concentrated Growth Factor with Dental Follicle Stem Cell Loading

“…The combination of DFCs with dentin matrix scaffolds can facilitate root regeneration . Moreover, the synergistic use of DFCs with nanofibers, silicon-based materials, and synthetic matrices markedly bolsters osteogenic differentiation. , These findings underscore the potential of DFCs as seminal cells in the realm of bone tissue engineering.…”

Chitosan Hydrogel as Tissue Engineering Scaffolds for Vascular Regeneration Applications

“…Hydrogels can be prepared by physical and chemical crosslinking and are commonly used as injectable materials. These hydrogel scaffolds find extensive applications in various fields such as optical and fluid actuators, tissue engineering scaffolds, cell culture substrates, and drug delivery carriers [91][92][93].…”

Nanofibrous Microspheres: A Biomimetic Platform for Bone Tissue Regeneration

“…Within this landscape, nanofibrous microspheres (NFMS) have emerged as a promising biomimetic platform for bone tissue regeneration. These microspheres offer a high surface area-to-volume ratio and can be precisely engineered to mimic the nanostructure and biochemical cues of the native ECM. , Through their ability to support cell adhesion, proliferation, and differentiation, as well as facilitate the controlled release of bioactive molecules, NFMS present a versatile and effective approach for promoting bone healing . This work presents the first comprehensive review of NFMS specifically for bone tissue regeneration, an area previously unexplored in such depth.…”

“… 20 , 21 Through their ability to support cell adhesion, proliferation, and differentiation, as well as facilitate the controlled release of bioactive molecules, NFMS present a versatile and effective approach for promoting bone healing. 22 This work presents the first comprehensive review of NFMS specifically for bone tissue regeneration, an area previously unexplored in such depth. It highlights recent developments in NFMS technology, from design principles and cutting-edge fabrication methods to the critical features that confer biomimetic properties.…”