Large-leaf yellow tea attenuates high glucose-induced vascular endothelial cell injury by up-regulating autophagy and down-regulating oxidative stress

Wang, Pu; Huang, Yao; Ren, Jiayue; Rong, Yuezhao; Fan, Lili; Zhang, Peng; Zhang, Xueying; Xi, Junxiao; Mao, Shuying; Min, Su; Zhang, Baobao; Bao, Guan‐Hu; Wu, Feihua

doi:10.1039/d1fo03405g

Cited by 17 publications

(12 citation statements)

References 52 publications

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“…[7][8][9][10] The most important green tea constituents are catechins, a group of bioactive polyphenols. [11][12][13][14][15][16] Although several studies have described the antiaging effects of tea extract, [17][18][19][20][21] antiaging studies on tea components mostly focus on the major catechin, (−)-epigallocatechin gallate (EGCG). Nevertheless, these studies, which only produced mediocre antiaging results, have limited our understanding of the antiaging effects and the underlying material basis of green tea.…”

Section: Introductionmentioning

confidence: 99%

Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways

Gao

et al. 2022

Food Funct.

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Section: Introductionmentioning

confidence: 99%

Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways

Gao

et al. 2022

Food Funct.

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“…Moreover, a study reported that the protein level of p‐p70S6K, a mTORC1 downstream molecule, is elevated in the HG environment (Fan et al, 2017). In our previous study, upregulating the AMPK/mTOR signaling pathway could enhance autophagy via the AMPK‐dependent pathway in HG‐treated HUVECs (Wang et al, 2022). Our results are consistent with those of previous studies which reported that the protein expression levels of p‐mTOR and p‐p70S6K is increased and mTOR is activated in HG‐treated HUVECs.…”

Section: Discussionmentioning

confidence: 91%

“…Long‐term hyperglycaemia in diabetes can cause various cardiovascular diseases, such as atherosclerosis and cardiac hypertrophy. Vascular endothelial injury is the main factor in the development of diabetic vascular complications (Wang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Homoplantaginin attenuates high glucose‐induced vascular endothelial cell apoptosis through promoting autophagy via the AMPK/TFEB pathway

Fan

Zhang

Huang

et al. 2023

Phytotherapy Research

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Vascular endothelial cell (VEC) injury is a key factor in the development of diabetic vascular complications. Homoplantaginin (Hom), one of the main flavonoids from Salvia plebeia R. Br. has been reported to protect VEC. However, its effects and mechanisms against diabetic vascular endothelium remain unclear. Here, the effect of Hom on VEC was assessed using high glucose (HG)‐treated human umbilical vein endothelial cells and db/db mice. In vitro, Hom significantly inhibited apoptosis and promoted autophagosome formation and lysosomal function such as lysosomal membrane permeability and the expression of LAMP1 and cathepsin B. The antiapoptosis effect of Hom was reversed by autophagy inhibitor chloroquine phosphate or bafilomycin A1. Furthermore, Hom promoted gene expression and nuclear translocation of transcription factor EB (TFEB). TFEB gene knockdown attenuated the effect of Hom on upregulating lysosomal function and autophagy. Moreover, Hom activated adenosine monophosphate‐dependent protein kinase (AMPK) and inhibited the phosphorylation of mTOR, p70S6K, and TFEB. These effects were attenuated by AMPK inhibitor Compound C. Molecular docking showed a good interaction between Hom and AMPK protein. Animal studies indicated that Hom effectively upregulated the protein expression of p‐AMPK and TFEB, enhanced autophagy, reduced apoptosis, and alleviated vascular injury. These findings revealed that Hom ameliorated HG‐mediated VEC apoptosis by enhancing autophagy via the AMPK/mTORC1/TFEB pathway.

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“…It is important to research new applications of tea flowers for improving the economic value thereof. Large-leaf yellow tea (LYT) is one kind of yellow-type tea which has multiple health benefits, such as alleviating alcoholic liver disease and inhibiting adipose tissue hypertrophy ( Xu et al., 2021 ; Wang et al., 2022 ; Wu et al., 2022 ). However, LYT is made from old tea leaves and stems, leading to poor aroma and flavor or being unpopular.…”

Section: Resultsmentioning

confidence: 99%

“…In tea flowers, the abundance of volatiles, particularly the terpenoid compounds, was detected. Then, we analyzed the terpenoid biosynthesis in flowers, and the transcriptome data of developing leaves and stems was used for comparison as control, which was generated by our previous study (Zhang et al, 2022). Most early genes involved in terpenoid biosynthesis showed low expression levels in F1, then increased in F2 and F3, and then kept a high level in opening flowers (Supplementary Table S3).…”

Section: Tea Flowers Expressed Terpenoid Biosynthesis Genes For Diver...mentioning

confidence: 99%

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

Tang

Shen

et al. 2022

Front. Plant Sci.

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The flowers of tea plants (Camellia sinensis), as well as tea leaves, contain abundant secondary metabolites and are big potential resources for the extraction of bioactive compounds or preparation of functional foods. However, little is known about the biosynthesis and transcriptional regulation mechanisms of those metabolites in tea flowers, such as terpenoid, flavonol, catechins, caffeine, and theanine. This study finely integrated target and nontarget metabolism analyses to explore the metabolic feature of developing tea flowers. Tea flowers accumulated more abundant terpenoid compounds than young leaves. The transcriptome data of developing flowers and leaves showed that a higher expression level of later genes of terpenoid biosynthesis pathway, such as Terpene synthases gene family, in tea flowers was the candidate reason of the more abundant terpenoid compounds than in tea leaves. Differently, even though flavonol and catechin profiling between tea flowers and leaves was similar, the gene family members of flavonoid biosynthesis were selectively expressed by tea flowers and tea leaves. Transcriptome and phylogenetic analyses indicated that the regulatory mechanism of flavonol biosynthesis was perhaps different between tea flowers and leaves. However, the regulatory mechanism of catechin biosynthesis was perhaps similar between tea flowers and leaves. This study not only provides a global vision of metabolism and transcriptome in tea flowers but also uncovered the different mechanisms of biosynthesis and transcriptional regulation of those important compounds.

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Large-leaf yellow tea attenuates high glucose-induced vascular endothelial cell injury by up-regulating autophagy and down-regulating oxidative stress

Abstract: Vascular endothelial cells injury induced by high glucose (HG) plays an important role in the occurrence and development of diabetic vascular complications. Yellow tea has a protective effect on vascular...

Cited by 17 publications

References 52 publications

Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways

Two new catechins from Zijuan green tea enhance the fitness and lifespan of Caenorhabditis elegans via insulin-like signaling pathways

Homoplantaginin attenuates high glucose‐induced vascular endothelial cell apoptosis through promoting autophagy via the AMPK/TFEB pathway

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

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