Large-scale characterization of sex pheromone communication systems in<i>Drosophila</i>

Khallaf, Mohammed A.; Cui, Rongfeng; Weißflog, Jerrit; Svatoš, Aleš; Dweck, Hany K.M.; Valenzano, Dario Riccardo; Hansson, Bill S.; Knaden, Markus

doi:10.1101/2020.09.21.305854

Cited by 8 publications

(13 citation statements)

References 87 publications

(94 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within the 10 minutes, however, all of the flies had copulated except DGRP882 males paired with Z53 males. We did not find however, known "female" CHCs on the males, confirming that any changes to the males were not the result of female CHCs rubbing off of males (KHALLAF et al 2020b). Quantification of CHCs is described in the Supplemental Information.…”

Section: Cuticular Hydrocarbon Collection and Quantificationsupporting

confidence: 64%

“…Female-genotype-dependent courtship plasticity suggests that males require lineage-specific female cues for successful courtship (BARKER 1967;COBB AND JALLON 1990;SEEHOLZER et al 2018). CHCs can both stimulate and inhibit mating (FERVEUR 2005;GRILLET et al 2006;EJIMA et al 2007;KHALLAF et al 2020a;KHALLAF et al 2020b), and a strong difference exists in the female CHCs of Z-type and M-type females (COYNE et al 1999;DALLERAC et al 2000). The ability of males to change their courtship can alter their courtship in subsequent mating interactions, which are typically not considered in the study of speciation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Divergent selection on behavioral and chemical traits contributes to isolation between populations ofDrosophila melanogaster

Jin

Barbash

Castillo

2020

Preprint

View full text Add to dashboard Cite

Speciation is driven by traits that can act to prohibit mating between nascent lineages, including male courtship and female preference for male traits. Mating barriers involving these traits evolve quickly because there is strong selection on males and females to maximize reproductive success, and the tight co-evolution of mating interactions can lead to rapid diversification of sexual behavior. Using lineages of D. melanogaster that show strong asymmetrical reproductive isolation, we ask two key questions: which specific male traits are females selecting, and are these traits under divergent sexual selection? These questions have proven extremely challenging to answer, because even in closely related lineages males often differ in multiple traits related to mating behavior. We address these questions by estimating selection gradients for male courtship and cuticular hydrocarbons for two different female genotypes. We identify both specific behaviors and several cuticular hydrocarbons that are under divergent sexual selection and likely contribute to reproductive isolation. Additionally a subset of these traits are plastic; males adjust these traits based on the identity of the female genotype they interact with. These results suggest that even when male courtship is not fixed between lineages, ongoing selection can contribute to reproductive isolation.

show abstract

Section: Cuticular Hydrocarbon Collection and Quantificationsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Divergent selection on behavioral and chemical traits contributes to isolation between populations ofDrosophila melanogaster

Jin

Barbash

Castillo

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the atrophied ejaculation bulb of the D. suzukii male does not produce cVA (Dekker et al, 2015). Some Drosophila species do not produce but still detect cVA (Khallaf et al 2020). Electrophysiological recording revealed that the D. melanogaster and D. suzukii females both can able to detect cVA by olfactory neurons in antennal trichoid sensilla (Khallaf et al, 2020).…”

Section: Discussionmentioning

confidence: 96%

“…Some Drosophila species do not produce but still detect cVA (Khallaf et al 2020). Electrophysiological recording revealed that the D. melanogaster and D. suzukii females both can able to detect cVA by olfactory neurons in antennal trichoid sensilla (Khallaf et al, 2020). cVA bind to the DmOBP69a (Bentzur et al, 2018) and DmOBP76a (Billeter and Levine, 2015).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Zhan¹,

Dewer

et al. 2021

Heliyon

View full text Add to dashboard Cite

The fruit fly Drosophila suzukii is a fruit crop pest that causes a severe economic threat to soft summer fruit worldwide. The male sex pheromone, cis-vaccenyl acetate (cVA) has multiple functions in intra-species communication in Drosophila melanogaster, which is required in male to suppress male-male courtship. D. suzukii males do not produce cVA; however, the odorant receptor for cVA (Or67d) is still functional. The lack of cVA in D. suzukii casts the question of whether this pheromone might have been replaced by another compound similar to cVA that disrupts mating in D. suzukii. In order to address this question, we cloned two D. suzukii adult antenna-specific odorant-binding proteins (OBPs) DsOBP69a and DsOBP76a and aligned with their D. melanogaster orthologues. Moreover, we examined the binding properties of the newly identified recombinant proteins against 26 potential ligands including cVA, using the fluorescence-based ligand binding assay. The alignment showed that DsOBP69a and DsOBP76a, have six conserved cysteines and belong to the classic OBP family. Furthermore, our results revealed that cVA did not bind to DsOBP69a or DsOBP76a proteins. Interestingly, the floral odorant β-ionone and the bitter substance berberine chloride and coumarin displayed high binding ability. It is also worth noting that DsOBP69a and DsOBP76a have different affinities to (Z)-7-Tricosene that may reflect different functional roles. These findings suggest that DsOBP69a and DsOBP76a are potentially involved in olfaction and gustation of D. suzukii.

show abstract

The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

Borrero‐Echeverry

Solum

Trona

et al. 2021

Preprint

View full text Add to dashboard Cite

Specific mate communication and recognition underlies reproduction and hence speciation. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues.Our study provides new insights in Drosophila melanogaster premating olfactory communication, showing that female pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone cVA interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males.Twin variants of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons, and feed into the same glomerulus in the antennal lobe. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. That Z4-11Al mediates courtship in experienced males, not (R)- linalool, is probably due to courtship learning. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds.Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in to contrast to Z4-11Al. A blend of cVA and the food odour vinegar attracted females, while a blend of female pheromone Z4-11Al and vinegar attracted males instead.Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects.SummaryThe female-produced, species-specific volatile pheromone of D. melanogaster attracts both sexes from a distance, alone and in concert with food odorants, and elicits courtship in experienced males.

show abstract

Large-scale characterization of sex pheromone communication systems inDrosophila

Cited by 8 publications

References 87 publications

Divergent selection on behavioral and chemical traits contributes to isolation between populations ofDrosophila melanogaster

Divergent selection on behavioral and chemical traits contributes to isolation between populations ofDrosophila melanogaster

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

Contact Info

Product

Resources

About