Large-Scale Integration of All-Glass Valves on a Microfluidic Device

Yalikun, Yaxiaer; Tanaka, Yo

doi:10.3390/mi7050083

Cited by 39 publications

(23 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, poly(dimethylsiloxane) (PDMS) is the most commonly used membrane material due to its good optical transparency and high elasticity for large deformations [10]. Other materials, such as thermal plastic polymer [11,12], shape memory alloy [13,14], glass [15], and more are also used in certain situations. As for the deflection of the membrane, various mechanical [16,17], electrostatic [18,19], pneumatic [20,21], magnetic [22,23], piezoelectric [24], or thermal [25] mechanisms have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Passive Valve with Ultra-Low Threshold Pressure for High-Throughput Liquid Delivery

Zhang

Oseyemi

2019

Micromachines

View full text Add to dashboard Cite

The microvalve for accurate flow control under low fluidic pressure is vital in cost-effective and miniaturized microfluidic devices. This paper proposes a novel microfluidic passive valve comprising of a liquid chamber, an elastic membrane, and an ellipsoidal control chamber, which actualizes a high flow rate control under an ultra-low threshold pressure. A prototype of the microvalve was fabricated by 3D printing and UV laser-cutting technologies and was tested under static and time-dependent pressure conditions. The prototype microvalve showed a nearly constant flow rate of 4.03 mL/min, with a variation of ~4.22% under the inlet liquid pressures varied from 6 kPa to 12 kPa. In addition, the microvalve could stabilize the flow rate of liquid under the time-varying sinusoidal pressures or the square wave pressures. To validate the functionality of the microvalve, the prototype microvalve was applied in a gas-driven flow system which employed an air blower or human mouth blowing as the low-cost gas source. The microvalve was demonstrated to successfully regulate the steady flow delivery in the system under the low driving pressures produced by the above gas sources. We believe that this new microfluidic passive valve will be suitable for controlling fluid flow in portable microfluidic devices or systems of wider applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Microfluidic Passive Valve with Ultra-Low Threshold Pressure for High-Throughput Liquid Delivery

Zhang

Oseyemi

2019

Micromachines

View full text Add to dashboard Cite

show abstract

“…Each glass device had an orifice layer and a microchannel layer. First, we fabricated the microchannel layer using the following lithography method . The channel design was patterned onto a glass photomask by a mask generator (DL‐1000 Nano System Solution, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

et al. 2019

Self Cite

View full text Add to dashboard Cite

Imaging flow cytometry is a powerful tool by virtue of its capability for high‐throughput cell analysis. The advent of high‐speed optical imaging methods on a microfluidic platform has significantly improved cell throughput and brought many degrees of freedom to instrumentation and applications over the last decade, but it also poses a predicament on microfluidic chips. Specifically, as the throughput increases, the flow speed also increases (currently reaching 10 m/s): consequently, the increased hydrodynamic pressure on the microfluidic chip deforms the wall of the microchannel and produces detrimental effects lead to defocused and blur image. Here, we present a comprehensive study of the effects of flow‐induced microfluidic chip wall deformation on imaging flow cytometry. We fabricated three types of microfluidic chips with the same geometry and different degrees of stiffness made of polydimethylsiloxane (PDMS) and glass to investigate material influence on image quality. First, we found the maximum deformation of a PDMS microchannel was >60 μm at a pressure of 0.6 MPa, while no appreciable deformation was identified in a glass microchannel at the same pressure. Second, we found the deviation of lag time that indicating velocity difference of migrating microbeads due to the deformation of the microchannel was 29.3 ms in a PDMS microchannel and 14.9 ms in a glass microchannel. Third, the glass microchannel focused cells into a slightly narrower stream in the X‐Y plane and a significantly narrower stream in the Z‐axis direction (focusing percentages were increased 30%, 32%, and 5.7% in the glass channel at flow velocities of 0.5, 1.5, and 3 m/s, respectively), and the glass microchannel showed stabler equilibrium positions of focused cells regardless of flow velocity. Finally, we achieved the world's fastest imaging flow cytometry by combining a glass microfluidic device with an optofluidic time‐stretch microscopy imaging technique at a flow velocity of 25 m/s. © 2019 International Society for Advancement of Cytometry

show abstract

“…S1, Supporting Information) and the fabrication method of the glass device used in this study were the same as in our previous research. 35 The chip had a four-layer-bonded structure. Layer 1 was a layer (0.4 mm in thickness) with channels (150 μm width, 75 μm depth) fabricated by typical wet etching process.…”

Section: Device Fabricationmentioning

confidence: 99%

“…In our previous research, 110 computer-controlled commercial piezoelectric units purchased from KGS Corporation (Saitama, Japan) were used. 35 In this study, we used 11 individual piezoelectric units in the first line. The white pins were piezoelectric units with a diameter of 1 mm, and the pitches of these pins were 4.8 mm (horizontal) and 2.4 mm (vertical).…”

Section: System Structurementioning

confidence: 99%

“…The position control property of the piezoelectric unit was investigated in our previous paper. 35 This system consisted of three main parts: a PC (with a graphic user interface (GUI) installed), a commercial circuitboard-based controller that provided power and control signals, and a piezoelectric head containing 110 piezoelectric pin units. We could first design one or numerous graphic patterns to describe the locations and activating time sequence of the valves that we wanted to open and those that needed to be closed.…”

Section: System Structurementioning

confidence: 99%

See 1 more Smart Citation

Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Single cell analysis has gained attention as a means to investigate the heterogeneity of cells and amplify a cell with desired characteristics. However, obtaining a single cell from a large number of cells remains difficult because preparation of single-cell samples relies on conventional techniques such as pipetting that are labor intensive. In this study, we developed a system combining a 0.6-mm thin glass microfluidic device and machine vision approach to isolate single Euglena gracilis cells, as a model of microorganism with mobility, in a small/thin glass chamber. A single E. gracilis cell in a chamber was cultured for 4 days to monitor its multiplication. With this system, we successfully simplified preparation of single cells of interest and determined that it is possible to combine it with other analytical techniques to observe single cells continuously.

show abstract

Large-Scale Integration of All-Glass Valves on a Microfluidic Device

Cited by 39 publications

References 50 publications

Microfluidic Passive Valve with Ultra-Low Threshold Pressure for High-Throughput Liquid Delivery

Microfluidic Passive Valve with Ultra-Low Threshold Pressure for High-Throughput Liquid Delivery

Effects of Flow‐Induced Microfluidic Chip Wall Deformation on Imaging Flow Cytometry

Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells

Contact Info

Product

Resources

About