Large-scale isolation of monocyte using newly developed nylon-wool bag for high purity and high yield selections of human CD34 positive peripheral blood stem cells.

A rapid nylon-fiber syringe system to deplete CD14+ cells for positive selection of human blood CD34+ cells. Use of immunomagnetic microspheres

“…The subsequent CD34 + cells from mobilized peripheral blood can be achieved by depleting adherent cells, such as monocytes, from immunomagnetic positive selection achieved preparations of 91 ± 8% pure CD34 + cells with a 86 ± 23% the starting cell population. 15 In the present work, we searched for conditions for rapid depletion of monocytes, yield. The overall yield of CD34 + cells was 44 ± 11%, and the time required for all procedures was 5 h. There assessed as CD14 + cells, and a permitted frequency of residual CD14 + cells for further purification, from normal was a tight and an inverse correlation (P Ͻ 0.0001) between the frequency of CD14 + cells in the initial cell human blood mononuclear cells.…”