Large scale production of d-allose from d-psicose using continuous bioreactor and separation system

Rare sugar d-allulose: Potential role and therapeutic monitoring in maintaining obesity and type 2 diabetes mellitus

“…14 C-Labelled D-allulose was enzymatically synthesized (Morimoto et al, 2006) to study its absorption, distribution, and elimination after both intravenous and oral administration in…”

Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing d-allose from d-psicose

“…Moreover, a combination use of D-allose as a potent immunosuppressant with a low dose of FK506 significantly increased the rate of allograft survival with less tissue damage (Hossain et al 2000). The biological manufacture of D-allose has been studied using L-rhamnose isomerase (L-RI) from Pseudomonas stutzeri, which is the only reported enzyme (Bhuiyan et al 1998;Leang et al 2004a;Morimoto et al 2006;Menavuvu et al 2006). Therefore, the investigation and further development for other sources of enzymes to produce D-allose should be greatly needed.…”

Cloning, Expression, and Transcription Analysis ofL-Arabinose Isomerase Gene fromMycobacterium smegmatisSMDU

“…[17][18][19][20][21] We found that mass production of D-allose and D-psicose from D-psicose and D-fructose were achievable using L-rhamnose isomerase and D-tagatose 3-epimerase respectively, and that both of these enzymes showed broad substrate specificity on various kinds of sugars. 4,22) Meanwhile, L-AI (EC 5.3.1.4), which specifically recognizes the substrate owing to the configuration of the sugar structure, catalyzes the conversion of D-galactose to D-tagatose as well as the conversion of L-arabinose to L-ribulose. 10) This property might be explained by the structural similarity between L-arabinose and D-galactose, including a shared L-cis-hydroxyl configuration at C3-C4.…”

“…Recently, rare sugars increased attraction not only in the food industry but also in pharmaceutical and nutritional industries on account of multipurpose applications such as their use as reduced-calorie sweeteners, inhibitors of microbial growth, bulking agents, antioxidants, and nucleoside analogues. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] We are working mainly on the production of various kinds of rare sugars using microorganisms screened from a variety of natural habitats and their enzymes. Using these microorganisms, we are studying several aldose-ketose isomerases (ketol isomerases), such as Lfucose isomerase (D-arabinose isomerase), L-arabinose isomerase (L-AI), L-ribose isomerase, and L-rhamnose isomerase, which are applicable in the production of various rare sugars as well as D-tagatose 3-epimerase and polyol dehydrogenases.…”