Large scale propagation of BHK21 cells using the opticell culture system

Bognar, Ernest; Pugh, Gordon G.; Lydersen, Bjorn K.

doi:10.1007/bf01665877

Cited by 8 publications

References 5 publications

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Development of a single‐pass ceramic matrix bioreactor for large‐scale mammalian cell culture

Applegate

Stephanopoulos

1992

Biotech & Bioengineering

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A single-pass, plug-flow bioreactor has been developed in which oxygen is supplied to entrapped hybridoma cells via silicone tubes threaded through the square channels of a macroporous ceramic monolith. Oxygen diffuses from the gas phase, through the silicone tubing, across the open square channel, and into the pores of the ceramic wall where it is consumed by entrapped cells. Advantages of such a reactor include higher product yields, protection of cells from detrimental hydrodynamic effects, no internal moving parts to compromise asepsis, and simplicity of operation. A prototype bioreactor was constructed and operated over a range of residence times. A side-by-side experimental comparison with a conventional recycle bioreactor was performed by inoculating both bioreactors with cells from the same stock culture and feeding medium from the same reservoir. Final antibody titers were 80% higher in the single-pass bioreactor at a residence time of 200 minutes compared with those of the recycle bioreactor at a residence time of 800 minutes. A theoretical analysis of oxygen transport in this bioreactor is developed to highlight important design criteria and operating strategies for scale-up.

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Development of a single‐pass ceramic matrix bioreactor for large‐scale mammalian cell culture

Applegate

Stephanopoulos

1992

Biotech & Bioengineering

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High density cultivation of BSK cells on sintered alumina ceramic foam support

Lee¹,

Grace²,

Chow³

et al. 1991

Cytotechnology

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A perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5 x 10(9) BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offer the advantages of significant reduction in serum requirements and the potential for scale-up.

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