Large-scale propagation of Myrobolan (Prunus cerasifera) in RITA® bioreactors and ISSR-based assessment of genetic conformity

Nasri, Amèni; Baklouti, Emna; Romdhane, Amal Ben; Maâlej, M.; Schumacher, Heinz Martin; Drira, Noureddine; Fki, Lotfi

doi:10.1016/j.scienta.2018.10.016

Cited by 41 publications

(26 citation statements)

References 42 publications

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“…Pistachio, a tree important for its fruits, was cultured in RITA ® using juvenile and mature material of different species. As reported above for black lapacho and myrobolan , pistachio shows apical necrosis when its shoots are proliferated in semi‐solid medium . TIS decreased the incidence of this disorder (thereby increasing the number of shoots suitable for rooting), once hyperhydricity was controlled by adjusting the duration and frequency of immersion .…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 67%

“…Besides eucalyptus, other tree species showed better rooting in bioreactors than in SS, as reported for calabash tree , yerba mate , poplar , black lapacho , and myrobolan . In the two later cases, the use of liquid medium significantly reduced the apical necrosis detected when shoots grew in agar‐based medium, thereby increasing the number of shoots that could be rooted.…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 99%

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Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 67%

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 99%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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“…Akşehirli (2010) found higher number and length of shoots as well as leaf numbers of Myrobalan 29C rootstock in medium containing 1 mg L -1 BAP, 0.5 mg L -1 IBA, and 0.25 mg L -1 GA 3. Recently, Nasri et al (2019) also recorded the maximum shoot multiplication of Myrobalan 29C rootstock in culture medium including 1.0 mg L -1 BAP and 1 mg L -1 IBA. In contrast, it was reported that MS medium containing 2 mg L -1 BAP alone resulted in the best shoot multiplication in Prunus laurocerasus L. (cherry laurel) with 6.13 new shoots and 3.26 cm formed shoot lengths (Sulusoglu and Cavusoglu, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The diverse concentrations of plant growth regulators (PGRs) and mineral elements affect the in vitro propagation of clonal rootstock. There are limited in vitro studies on the micropropagation of Myrobalan 29C rootstocks (Arbeloa et al, 2009;Akşehirli, 2010;Movsiuw, 2011;Plopa et al, 2012;Özbek et al, 2014;Shabani et al, 2015;Nasri et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock

Güney¹

2019

Turk J Agric For

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Stone fruits are well known for their high nutritional value. Therefore, in horticulture, the micropropagation of suitable rootstocks is vital for their cultivation. The aim of the present study was to improve the micropropagation protocol of Myrobalan 29C (Prunus cerasifera Ehrh.) rootstock using shoot-tip culture. Murashige and Skoog (MS) basal medium containing 2 mg L-1 6-benzylaminopurine (BAP) and 0.05 mg L-1 gibberellic acid (GA 3) resulted in the highest number (14.3) of multiple shoots. However, a greater shoot length (2.0 cm) was attained when GA 3 was excluded from the MS medium and the concentration of BAP was reduced to 1 mg L-1. Root induction was best in ½MS medium containing 0.5 mg L-1 indole-3-butyric acid (IBA), 0.5 mg L-1 α-naphthaleneacetic acid, and 10 mL L-1 (≈13 mg L-1 Fe) ethylenediamine di-2-hydroxyphenyl acetate ferric with 7.0 roots per explant. On the other hand, the longest root (12.5 cm) was obtained from increased concentration to 1 mg L-1 of IBA. The establishment of a well-defined micropropagation protocol will lead to further biotechnological improvement of this crop.

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“…Cytokinin is necessary in cultured cells for the initiation of mitosis, promoting cell division and differentiation (Kieber & Schaller, 2018 ;Lustinec et al, 2014;Nasri et al, 2019;Takahashi & Umeda, 2014). However, higher concentrations of BA adversely affected cell proliferation in the liquid medium, as indicated by a reduced FW and DW of cells in the culture.…”

Section: Ec Proliferation In An Air-lift Bioreactor Culturementioning

confidence: 99%

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

Agisimanto¹,

Normah

Ibrahim

2019

Agrivita.J.Agr.Sci

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Embryogenic cell (EC) growth and proliferation as well as somatic embryo induction were examined in a bioreactor culture using Murashige and Skoog basal medium particularly for the influence of 6-benzyladenine (BA) concentration, initial cell density and aeration rate. Embryogenic callus was induced from seeds of Citrus reticulata Blanco cv. Madu. The cell suspension in a 3-L bioreactor exhibited maximum cell growth following the addition of 1.5 mg/l of BA. The fresh weight (FW) of the cells after 28 days of growth was found to increase from an initial of 5.5 g cell culture to 57.3 g, a 10.4-fold increase and the maximum growth rate (GR) of the cells (0.33 g/day) was attained by the 7 th day of culture. In the cell density experiment, ECs at a concentration of 5.5 g/l constituted the most effective inoculum, reaching the highest GR of ECs (0.52 g/ day), again by the 7 th day of culture. In the aeration experiment, the highest EC GR of 2.6 g/day was obtained at the maximum aeration rate of 1.5 vvm (air volume medium/ volume/min). After 28 days of somatic embryogenesis, 79% of ECs became somatic embryos, of which 29% were at cotyledonary stage.

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Large-scale propagation of Myrobolan (Prunus cerasifera) in RITA® bioreactors and ISSR-based assessment of genetic conformity

Cited by 41 publications

References 42 publications

Use of bioreactor systems in the propagation of forest trees

Use of bioreactor systems in the propagation of forest trees

Development of an in vitro micropropagation protocol for Myrobalan 29C rootstock

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

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