Large scale purification of puerarin from Puerariae Lobatae Radix through resins adsorption and acid hydrolysis

Guo, Hai-dong; Chen, Jiguang; Shangguang, Xin-Cheng; Guo, Yu-Xian

doi:10.1016/j.jchromb.2014.12.014

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…Natural products from Z. officinale are used as spices in the food industry and in medicine, and extracts are known to inhibit XOD . Extracts of S. baicalensis and P. lobata have been shown to possess anti‐inflammation and antioxidation activities. In this study, we screened extracts from these plants using the method described above, and chromatograms are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Screening inhibitors of xanthine oxidase from natural products using enzyme immobilized magnetic beads by high‐performance liquid chromatography coupled with tandem mass spectrometry

Wang

et al. 2017

J of Separation Science

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In this study, high-performance liquid chromatography coupled with tandem mass spectrometry was used to assess the results of bioactive compound screening from natural products using immobilized enzyme magnetic beads. We compared three commercial magnetic beads with modified amino, carboxy, and N-hydroxysuccinimide groups, respectively. Amino magnetic beads performed best for immobilization and were selected for further experiments. Xanthine oxidase was immobilized on amino magnetic beads and applied to screen potential inhibitors in fresh Zingiber officinale Roscoe, extracts of Scutellaria baicalensis Georgi, and Pueraria lobata Ohwi. In total, 12 potential xanthine oxidase ligands were identified from fresh Zingiber root and Scutellaria root extracts, of which eight were characterized and the concentration required for 50% inhibition was determined. Preliminary structure-function relationships were discussed based on these results. A convenient and effective method was therefore developed for the identification of active compounds from complex natural product mixtures.

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Section: Resultsmentioning

confidence: 99%

Screening inhibitors of xanthine oxidase from natural products using enzyme immobilized magnetic beads by high‐performance liquid chromatography coupled with tandem mass spectrometry

Wang

et al. 2017

J of Separation Science

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“…Langmuir and Freundlich equations are the two theoretical models frequently used for analysis of adsorption from solution. The Langmuir model describes a monolayer adsorption based on homogeneous and finite‐sorption‐site assumptions, while Freundlich model is suited for both monolayer and multilayer adsorption on heterogeneous surfaces (Guo et al., ). Table listed the simulated parameters through the experimental data for both models.…”

Section: Resultsmentioning

confidence: 99%

“…Langmuir and Freundlich models were used to simulate the adsorption isotherm data. The models are expressed as follows (Guo, Zhang, Chen, Shangguang, & Guo, ; Sandhu & Gu, ):…”

Section: Methodsmentioning

confidence: 99%

Purification of total flavonoids from Rhizoma Smilacis Glabrae through cyclodextrin‐assisted extraction and resin adsorption

Zhang

Zheng

2019

Food Science & Nutrition

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Flavonoids are the main bioactive components responded for the health promoting effects of Rhizoma Smilacis Glabrae (RSG), an herbal material used in many functional food of China. An eco‐friendly method with β‐cyclodextrin (β‐CD)‐assisted extraction and resin adsorption/desorption was developed for total flavonoids purification from RSG. Because of complexes formation between flavonoids and β‐CD, aqueous solution was used for extraction instead of ethanol. The CD‐assisted extraction was optimized through defining optimal CD species, concentration, extraction temperature, and time. The adsorption property of eight macroporous resins on astilbin was compared by adsorption kinetics and capacity. All resins could reach the adsorption equilibrium within 2 hr. Further analyzed by Langmuir and Freundlich models, H103 resin with the best adsorption capacity was selected. The desorption property of different ethanol–water solution was compared. Results showed that by using 75% ethanol, astilbin could be well desorbed from the resin with the recovery of 96.3%. Because of complexes formation, the presence of β‐CD would slightly decrease the adsorption rate and capacity of H103 with concentration dependent. In dynamic adsorption, decreasing the flow rate could minish the effects of β‐CD. The developed method was successfully used for total flavonoids purified from RSG. The yield of purified product was 8.78%, with astilbin and total flavonoids content of 363.8 and 505.7 mg/g, respectively. The purity was 1.74 times increased with the recovery of 94.38% compared with the extract obtained directly through 50% ethanol extraction.

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“…In recent years, various methods for extracting flavonoids have been developed, such as organic solvent extraction, ultrasound-assisted extraction, microwave extraction, and supercritical fluid extraction [19]. However, some of these methods suffer from the shortcomings of needing specific costly instrumentations, consuming a large amount of organic solvents, low output, or are unsuitable on an industrial scale [20]. To minimize the use of organic solvents and simplify the operating procedure, many other extraction methods have been developed in recent years.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Six Isoflavones from Puerariae Lobatae Radix by CPE-HPLC and Effect of Puerarin on Tyrosinase Activity

Song

Zhang

et al. 2020

Molecules

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Tyrosinase inhibitors with excellent inhibitory activities and lower side effects have promising applications in the fields of medicine, agriculture, food sciences and cosmetics. In this study, a method for simultaneous separation and determination of six target compounds (puerarin, daidzin, genistein, daidzein, genistin, and formononetin) in Puerariae Lobatae Radix was established by cloud point extraction (CPE) and concentration combined with high performance liquid chromatography (HPLC). To achieve high extraction yields, an ultrasound-assisted extraction method was developed based on a salt-modified Triton X-100 system. The optimal extraction conditions are: surfactant Triton X-100 concentration 0.07 g/mL, liquid-solid ratio 80:1 (mL/g), NaCl addition amount 0.6 g, equilibrium time 40 min, equilibrium temperature 70 °C. Under the optimal conditions, the total maximum extraction yield of the six target isoflavones reached 8.92 mg/g. Using l-tyrosine and l-dopa as substrates, the effects of puerarin on the monophenolase and diphenolase activity of tyrosinase activity were investigated by the enzyme kinetics method. The results showed that puerarin inhibited monophenolase activity with an IC50 of 0.537 mg/mL and activated diphenolase activity. The inhibition type of puerarin on monophenolase and the activation type of puerarin on diphenolase were analyzed by Lineweaver-Burk plots which show that puerarin showed mixed inhibition on monophenolase and mixed activation on diphenolase. Therefore, puerarin can be used as both a tyrosinase inhibitor and a tyrosinase activator.

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Large scale purification of puerarin from Puerariae Lobatae Radix through resins adsorption and acid hydrolysis

Cited by 14 publications

References 30 publications

Screening inhibitors of xanthine oxidase from natural products using enzyme immobilized magnetic beads by high‐performance liquid chromatography coupled with tandem mass spectrometry

Screening inhibitors of xanthine oxidase from natural products using enzyme immobilized magnetic beads by high‐performance liquid chromatography coupled with tandem mass spectrometry

Purification of total flavonoids from Rhizoma Smilacis Glabrae through cyclodextrin‐assisted extraction and resin adsorption

Simultaneous Determination of Six Isoflavones from Puerariae Lobatae Radix by CPE-HPLC and Effect of Puerarin on Tyrosinase Activity

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