Large-Scale Studies on Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Food Animals in Developed Areas of Eastern China

Ma, Jian-Gang; Zhou, Wei; Wu, Jing; Liu, Xiaofeng; Lin, Jiahui; Ji, Xiaofeng; Lin, Hui; Wang, Jianmei; Jiang, Han; Zhou, Qianjin; Zhao, Guoping; Yang, Hua; Tang, Biao

doi:10.1128/spectrum.02015-22

Cited by 34 publications

(26 citation statements)

References 42 publications

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“…To understand the current situation of AMR of E. coli , we analyzed AMR, MLST and virulence genes of E. coli isolated from anal swab samples of pigs and chickens in farms in Lishui, Quzhou and Jinhua, Zhejiang Province, Eastern China. In this study, relatively high AMR rates of FFC (79.56%), SXT (85.64%), SF (95.03%), SPT (88.40%) and AMP (84.53%) were detected that were consistent with the previously published results ( Ma et al, 2022 ; Peng Z. et al, 2022 ). At the same time, the AMR rates of A/C (3.31%), MEM (2.76%), CL (5.52%) and CAZ (7.18%) were low.…”

Section: Discussionsupporting

confidence: 93%

“…The recent emergence and rapid increase of multi-drug resistant (MDR, resistance to more than three kinds of antibiotics) bacteria have caused public concern, represented by Escherichia coli resistant to carbapenem, colistin and tigecycline which were recognized as the last line of resort ( Li et al, 2017 ; He et al, 2019 ; Ma et al, 2022 ). Due to incorrect use and misuse of antibiotics, the spread of antimicrobial resistance (AMR) is accelerating ( Wang et al, 2022 ; Tang et al, 2022a , b ).…”

Section: Introductionmentioning

confidence: 99%

“…E. coli is a commonly used AMR indicator in human and food animal ( Brisola et al, 2019 ; Ma et al, 2022 ). There have been many reports on the study in E. coli and monitoring of the spread of AMR.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

et al. 2022

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Escherichia coli is considered an opportunistic pathogen and an indicator for antimicrobial resistance (AMR) monitoring. Despite many reports on its AMR monitoring, studies based on genome-based analysis of AMR genes are still insufficient. Here, 181 E. coli strains were isolated from anal swab samples collected from pigs and chickens of animal farms located in Eastern China and sequenced through the Illumina platform. The results showed that 87.85% (159/181) of the E. coli isolates were multidrug-resistant (MDR). Ampicillin (AMP)- spectinomycin (SPT)- tetracycline (TET)- florfenicol (FFC)- sulfisoxazole (SF)- trimethoprim/sulfamethoxazole (SXT) was the predominant AMR pattern. By whole-genome sequencing, we found that ST10 (10.49%, 19/181) and ST48 (7.18%, 13/181) were major sequence types. IncFIB and IncX1 were the most prevalent plasmid replicons. The AMR genes blaNDM-5 (1.10%, 2/181), mcr-1 (1.10%, 2/181), tet(X4) (1.10%, 2/181), and cfr (6.08%, 2/181) were also found in these isolates. In addition, among the 169 virulence genes detected, we identified astA (37.02%, 67/181), hlyA (1.66%, 3/181), hlyB (1.66%, 3/181) and hlyD (1.66%, 3/181), which were closely related to heat-stable enterotoxin 1 and α-hemolysin. In addition, there were 33 virulence genes associated with the iron uptake system, and 46 were adhesion-related genes. Our study highlighted the need for routine surveillance of AMR with advanced genomic approaches, providing up-to-date data on the prevalence of AMR for the development and execution of antimicrobial stewardship policy.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

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“…S1-PFGE was used to detect plasmid size in strains performed as previously described ( Ma et al, 2022a , b ; Tang et al, 2022b ). In brief, the plugs were made from the fresh cultures embedded in 2% gold agarose and lysed with cell lysis buffer.…”

Section: Methodsmentioning

confidence: 99%

“…The tigecycline still exhibits antibacterial activities in the bacteria containing the earlier tetracyclines resistance genes. The plasmid-mediated tet (X3) and tet (X4) genes conferring tigecycline resistance were discovered in various Gram-negative bacteria, including carbapenem-resistant and colistin-resistant bacterial strains ( He T. et al, 2019 ; Sun et al, 2019 ; Tang et al, 2022a ; Ma et al, 2022b ). The tet (X) variant-positive isolates from animals, retail meat, and humans have been identified ( Zheng et al, 2020 ; Tang et al, 2021 ; Umar et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine

Wang

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et al. 2023

Front. Microbiol.

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The tigecycline resistance gene tet(X4) was widespread in various bacteria. However, limited information about the plasmid harboring the tet(X4) gene spread among the different species is available. Here, we investigated the transmission mechanisms of the tet(X4) gene spread among bacteria in a pig farm. The tet(X4) positive Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Enterobacter hormaeche were identified in the same farm. The whole genome sequencing (WGS) analysis showed that the K. pneumoniae belonged to ST727 (n = 11) and ST3830 (n = 1), E. cloacae and E. hormaeche belonged to ST524 (n = 1) and ST1862 (n = 1). All tet(X4) genes were located on the IncHI1 plasmids that could be conjugatively transferred into the recipient E. coli C600 at 30°C. Moreover, a fusion plasmid was identified that the IncHI1 plasmid recombined with the IncN plasmid mediated by ISCR2 during the conjugation from strains B12L to C600 (pB12L-EC-1). The fusion plasmid also has been discovered in a K. pneumoniae (K1L) that could provide more opportunities to spread antimicrobial resistance genes. The tet(X4) plasmids in these bacteria are derived from the same plasmid with a similar structure. Moreover, all the IncHI1 plasmids harboring the tet(X4) gene in GenBank belonged to the pST17, the newly defined pMLST. The antimicrobial susceptibility testing was performed by broth microdilution method showing the transconjugants acquired the most antimicrobial resistance from the donor strains. Taken together, this report provides evidence that IncHI1/pST17 is an important carrier for the tet(X4) spread in Enterobacteriaceae species, and these transmission mechanisms may perform in the environment.

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Farm to table: colistin resistance hitchhiking through food

et al. 2023

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Large-Scale Studies on Antimicrobial Resistance and Molecular Characterization of Escherichia coli from Food Animals in Developed Areas of Eastern China

Abstract: Pigs and chickens are the most common food animals that are the important vectors for spreading antimicrobial-resistant pathogens among animals and humans. Limited systematic AMR monitoring of these food animal origin bacteria had been reported, especially in developed areas of China.

Cited by 34 publications

References 42 publications

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine

Farm to table: colistin resistance hitchhiking through food

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