2015
DOI: 10.1128/aem.03157-14
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Large-Scale 13 C Flux Profiling Reveals Conservation of the Entner-Doudoroff Pathway as a Glycolytic Strategy among Marine Bacteria That Use Glucose

Abstract: e Marine bacteria form one of the largest living surfaces on Earth, and their metabolic activity is of fundamental importance for global nutrient cycling. Here, we explored the largely unknown intracellular pathways in 25 microbes representing different classes of marine bacteria that use glucose: Alphaproteobacteria, Gammaproteobacteria, and Flavobacteriia of the Bacteriodetes phylum. We used 13 C isotope experiments to infer metabolic fluxes through their carbon core pathways. Notably, 90% of all strains stu… Show more

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Cited by 71 publications
(67 citation statements)
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“…A recent detailed survey of a wide range of marine bacteria found that most of them had the required genes to metabolize glucose using three different pathways, namely the Entner-Doudoroff (ED), the Embden-Meyerhof-Parnas (EMP) and the pentose-phosphate pathway (PPP) [42]. 13 C flux-profiling experiments showed that all of them catabolized glucose using either ED or EMP, sometimes complemented by PPP.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…A recent detailed survey of a wide range of marine bacteria found that most of them had the required genes to metabolize glucose using three different pathways, namely the Entner-Doudoroff (ED), the Embden-Meyerhof-Parnas (EMP) and the pentose-phosphate pathway (PPP) [42]. 13 C flux-profiling experiments showed that all of them catabolized glucose using either ED or EMP, sometimes complemented by PPP.…”
Section: Resultsmentioning
confidence: 99%
“…elongata is able to grow on lactate [9]. Another peculiarity is that the enzymes for both ED and EMP pathways were detected in the proteomic analysis unlike the cases studied by Klingner and co-workers [42]. However, only the expression of the ED-pathway is up regulated with increasing salinity (phosphogluconate dehydratase, 2-keto-3-deoxyphosphogluconate aldolase), while none of the enzymes participating in the EMP-pathway and the pentose-phosphate pathway are significantly regulated by salt.…”
Section: Resultsmentioning
confidence: 99%
“…Thioredoxin also reduces the cytoplasmic methionine sulfoxide reductases MsrA and MsrB, which repair oxidized proteins (Atack and Kelly, 2008). Thus, ED-positive isolates may be better able to cope with oxidative stress than ED-negative isolates; this has been shown to be the case experimentally in Pseudomonas putida (Chavarria et al, 2013) and in a range of marine bacteria (Klingner et al, 2015). Secondly, the absence of Pfk but the presence of the remaining EMP pathway enzymes in campylobacters means that the ED reactions are not “linear.” Instead, the metabolism of glucose in ED-positive isolates could occur via the “cyclic” fusion of the ED and EMP reactions, as has been shown in Pseudomonas spp., which interestingly also lack Pfk (Nikel et al, 2015).…”
Section: Discussionmentioning
confidence: 95%
“…The Entner-Doudoroff Pathway (EDP) is a well conserved pathway in the Pseudomonas genus [116,117], whose members lack a complete EMPP, and has been shown to be preferentially utilized by glucose consuming marine bacteria and to correlate with oxidative stress tolerance [118]. The EDP was postulated to have selective advantages based on the observation of lowered fitness when Fructose-bisphosphate aldolase was introduced in a related species, Pseudomonas putida [119].…”
Section: Metabolism and Pathogenicitymentioning
confidence: 99%