Large disturbances frequently happen in isolated AC/DC Hybrid Microgrids. Unfortunately, constant power loads (CPLs) with negative impedance characteristics are equivalent to positive feedback, resulting in an increase in large disturbances. The system can easily become unstable. Consequently, large signal stability criteria are proposed in this paper. Combined with a three-dimensional region of asymptotic stability (3D RAS) method for islanded AC/DC Hybrid Microgrids, important parameters to increase stability margins were determined. Firstly, mixed potential theory was used to derive a large-signal stability criterion. The criteria gave constraints on filtering parameters, CPL power, power of the battery to charge and discharge, AC resistive loads, and DC bus voltage. Then, Lyapunov functions were constructed, and the Lasalle invariance principle was adopted to achieve 3D RAS. When large disturbances emerged, and simultaneously voltage and current varied in 3D RAS, the system always obtained stability and reached new steady-state equilibrium points. Finally, according to comparisons, bigger capacitances of the DC bus capacitor and the AC capacitor, larger battery discharging power and smaller charging power could significantly increase stability margins of islanded Microgrids. Simulations and experimental data have shown that the large signal stability criteria and the 3D RAS work.