Larvae of the small white butterfly, <i>Pieris rapae</i>, express a novel serotonin receptor

Qi, Yixin; Xia, Ren-ying; Wu, Ya-su; Stanley, David; Huang, Jia; Yè, Gōngyín

doi:10.1111/jnc.12940

Cited by 28 publications

(21 citation statements)

References 49 publications

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“…This receptor also occurs in the parasitic nematode Haemonchus contortus and is predicted to be present in several additional nematodes (Komuniecki et al, 2012;Beech et al, 2013), but it has not been reported in other invertebrate clades. In the butterfly P. rapae, a novel receptor has been described by Qi et al (2014) that is proposed to belong to a new family of receptors designated 5-HT 8 . 5-HT activation of this receptor in HEK293 cells induced an increase in Ca 2+ , but the sequence and pharmacology do not resemble 5-HT 2 or other insect 5-HT receptors.…”

Section: Rnai Knockdownmentioning

confidence: 99%

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Tierney

2018

Journal of Experimental Biology

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Invertebrate receptors for the neurotransmitter serotonin (5-HT) have been identified in numerous species from diverse phyla, including Arthropoda, Mollusca, Nematoda and Platyhelminthes. For many receptors, cloning and characterization in heterologous systems have contributed data on molecular structure and function across both closely and distantly related species. This article provides an overview of heterologously expressed receptors, and considers evolutionary relationships among them, classification based on these relationships and nomenclature that reflects classification. In addition, transduction pathways and pharmacological profiles are compared across receptor subtypes and species. Previous work has shown that transduction mechanisms are well conserved within receptor subtypes, but responses to drugs are complex. A few ligands display specificity for different receptors within a single species; however, none acts with high specificity in receptors across different species. Two non-selective vertebrate ligands, the agonist 5-methoxytryptamine and antagonist methiothepin, are active in most receptor subtypes in multiple species and hence bind very generally to invertebrate 5-HT receptors. Future challenges for the field include determining how pharmacological profiles are affected by differences in species and receptor subtype, and how function in heterologous receptors can be used to better understand 5-HT activity in intact organisms.

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Section: Rnai Knockdownmentioning

confidence: 99%

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Tierney

2018

Journal of Experimental Biology

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“…Furthermore, these G protein-coupled 5-HT receptors are mainly classified in three groups: (1) 5-HT1 and 5-HT5 receptors that couple preferentially to Gi proteins and inhibit cAMP synthesis, (2) 5-HT2 receptors that activate Gq proteins, which mediate the hydrolysis of inositol phosphates and cause a subsequent increase in cytosolic Ca 2+ , and (3) 5-HT4, 5-HT6, and 5-HT7 receptors that couple to Gs proteins and promote cAMP formation1819. Additionally, an extra novel serotonin receptor (named Pr5-HT8) has been identified from larval Pieris rapae and, and represents the first recognized member of a novel 5-HT receptor class with a unique pharmacological profile20. In marine invertebrates or dormancy animals, only several serotonin receptors have been identified from the barnacle, Balanus amphitrite Darwin21 and the marine mollusc Aplysia 2223, however, the 5-HT receptors and their physiological functions in marine invertebrates still remain largely unknown.…”

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confidence: 99%

Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka)

Wang

Yang

Zhou

et al. 2017

Sci Rep

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Serotonin (5-HT) is an important neurotransmitter and neuromodulator that controls a variety of sensory and motor functions through 5-HT receptors (5-HTRs). The 5-HT4R subfamily is linked to Gs proteins, which activate adenylyl cyclases (ACs), and is involved in many responses in peripheral organs. In this study, the 5-HT4R from Apostichopus japonicus (Aj5-HT4R) was identified and characterised. The cloned full-length Aj5-HT4R cDNA is 1,544 bp long and contains an open reading frame 1,011 bp in length encoding 336 amino acid proteins. Bioinformatics analysis of the Aj5-HT4R protein indicated this receptor was a member of class A G protein coupled receptor (GPCR) family. Further experiments using Aj5-HT4R-transfected HEK293 cells demonstrated that treatment with 5-HT triggered a significant increase in intracellular cAMP level in a dose-dependent manner and induced a rapid internalisation of Aj5-HT4R fused with enhanced green fluorescent protein (Aj5-HT4R-EGFP) from the cell surface into the cytoplasm. In addition, the transcriptional profiles of Aj5-HT4R in aestivating A. japonicas and phosphofructokinase (AjPFK) in 5-HT administrated A. japonicus have been analysed by real-time PCR assays. Results have led to a basic understanding of Aj5-HT4R in A. japonicus, and provide a foundation for further exploration of the cell signaling and regulatory functions of this receptor.

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“…An additional receptor, MOD-1, a serotonin-gated ion channel, was found in C. elegans and exhibited similarities to the mammalian 5-HT3 receptor, but with differences in function ( 76 ). Qi et al ( 77 ) reported a novel receptor in the butterfly Pieris rapae , that was classified into a new family of receptors designated as 5-HT8. As in vertebrates, serotonin activates different downstream signaling pathways in order to control levels of second messengers, specifically adenylate cyclase activity and cAMP production (inhibited by Gi-coupled 5-HT1-like receptors, but stimulated by Gs-coupled 5-HT7-like receptors) or phospholipase C, and subsequently increases Ca 2+ (stimulated by Gq-coupled 5-HT2-like receptors) ( 75 ), to exert both inhibitory and excitatory effects.…”

Section: Serotoninmentioning

confidence: 99%

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

2020

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Vitellogenesis in crustaceans is an energy-consuming process. Though the underlying mechanisms of ovarian maturation in decapod Crustacea are still unclear, evidence indicates the process to be regulated by antagonistically-acting inhibitory and stimulating factors specifically originating from X-organ/sinus gland (XO/SG) complex. Among the reported neuromediators, neuropeptides belonging to the crustacean hyperglycemic hormone (CHH)-family have been studied extensively. The structure and dynamics of inhibitory action of vitellogenesis-inhibiting hormone (VIH) on vitellogenesis have been demonstrated in several species. Similarly, the stimulatory effects of other neuropeptides of the CHH-family on crustacean vitellogenesis have also been validated. Advancement in transcriptomic sequencing and comparative genome analysis has led to the discovery of a large number of neuromediators, peptides, and putative peptide receptors having pleiotropic and novel functions in decapod reproduction. Furthermore, differing research strategies have indicated that neurotransmitters and steroid hormones play an integrative role by stimulating neuropeptide secretion, thus demonstrating the complex intertwining of regulatory factors in reproduction. However, the molecular mechanisms by which the combinatorial effect of eyestalk hormones, neuromediators and other factors coordinate to regulate ovarian maturation remain elusive. These multifunctional substances are speculated to control ovarian maturation possibly via the autocrine/paracrine pathway by acting directly on the gonads or by indirectly exerting their stimulatory effects by triggering the release of a putative gonad stimulating factor from the thoracic ganglion. Acting through receptors, they possibly affect levels of cyclic nucleotides (cAMP and cGMP) and Ca 2+ in target tissues leading to the regulation of vitellogenesis. The “stimulatory paradox” effect of eyestalk ablation on ovarian maturation continues to be exploited in commercial aquaculture operations, and is outweighed by the detrimental physiological effects of this procedure. In this regard, the development of efficient alternatives to eyestalk ablation based on scientific knowledge is a necessity. In this article, we focus principally on the signaling pathways of positive neuromediators and other factors regulating crustacean reproduction, providing an overview of their proposed receptor-mediated stimulatory mechanisms, intracellular signaling, and probable interaction with other hormonal signals. Finally, we provide insight into future research directions on crustacean reproduction as well as potential applications of such research to aquaculture technology development.

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Larvae of the small white butterfly, Pieris rapae, express a novel serotonin receptor

Cited by 28 publications

References 49 publications

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka)

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

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