Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

Zahoor, Zahida; Davies, Angela J.; Kirk, Ruth; Rollinson, David; Walker, Anthony J.

doi:10.1007/s12192-010-0176-z

Cited by 45 publications

(46 citation statements)

References 65 publications

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“…glabrata hemocytes after incubation with excretory/secretory products (ES) from S . mansoni [77], which was linked to a parasite strategy to manipulate the snail’s immune response. Conversely, other studies have described an up-regulation of the expression levels of this protein as a result of parasite infection [34, 36, 78], so the role of these proteins in the snail’s defense against pathogens is not entirely clear.…”

Section: Discussionmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Cambodia). Chronic O. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma (a fatal type of bile duct cancer). Infectivity of the parasite in the snail host is strongly correlated with destruction of helminths by the snail’s innate immune system, composed of cellular (hemocyte) and humoral (plasma) defense factors. To better understand this important host-parasite interface we applied sequential window acquisition of all theoretical spectra mass spectrometry (SWATH-MS) to identify and quantify the proteins from the hemolymph of B. siamensis goniomphalos experimentally infected with O. viverrini and compare them to non-infected snails (control group). A total of 362 and 242 proteins were identified in the hemocytes and plasma, respectively. Of these, 145 and 117 proteins exhibited significant differences in expression upon fluke infection in hemocytes and plasma, respectively. Among the proteins with significantly different expression patterns, we found proteins related to immune response (up-regulated in both hemocyte and plasma of infected snails) and proteins belonging to the structural and motor group (mostly down-regulated in hemocytes but up-regulated in plasma of infected snails). The proteins identified and quantified in this work will provide important information for the understanding of the factors involved in snail defense against O. viverrini and might facilitate the development of new strategies to control O. viverrini infection in endemic areas.

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Section: Discussionmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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“…2), larvae release a mixture of molecules into the surrounding medium including an array of glycoproteins (Guillou et al 2007; Wu et al 2009). Hemocytes, when exposed to these larval transformation products (or LTPs; Wu et al 2009), exhibit altered in vitro motility, phagocytic activity, and capacity to encapsulate sporocysts (Bayne 2009; Yoshino and Coustau 2011) as well as modulation of specific proteins (e.g., HSP70; Zahoor et al 2010) that are associated with schistosome resistance in snails (Ittiprasert et al 2009; Ittiprasert and Knight 2012). To better understand how LTPs interact with snail cells at the molecular level, Bge cells were treated with LTPs of S. mansoni and Echinostoma caproni Richard, 1964 and transcript expression was evaluated.…”

Section: Molluscan Cell Linesmentioning

confidence: 99%

Molluscan cells in culture: primary cell cultures and cell lines

2013

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata Say, 1818 embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host-parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.

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“…Additionally, unlike the Lockyer et al study where constitutive expression of Hsp 70 transcript was not observed in either resistant or susceptible adult snails, we showed that the expression of Hsp 70 occurs at similar levels in both normal resistant and susceptible juvenile snails. Furthermore, another study done using hemocytes collected from parasite exposed adult resistant and susceptible snails, showed down regulation of the Hsp 70 protein occurs in hemocytes of both these snails following infection, with more suppression of the transcript in susceptible than in resistant adult snails [23]. Thus, from the above studies, it is clear that there are major discrepancies concerning the expression of Hsp 70 between juvenile and adult resistant and susceptible snails, either with, or without infection, and also from hemocytes removed from infected adult resistant and susceptible snails.…”

Section: Introductionmentioning

confidence: 97%

Reversing the Resistance Phenotype of the Biomphalaria glabrata Snail Host Schistosoma mansoni Infection by Temperature Modulation

Ittiprasert

Knight

2012

PLoS Pathog

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Biomphalaria glabrata snails that display either resistant or susceptible phenotypes to the parasitic trematode, Schistosoma mansoni provide an invaluable resource towards elucidating the molecular basis of the snail-host/schistosome relationship. Previously, we showed that induction of stress genes either after heat-shock or parasite infection was a major feature distinguishing juvenile susceptible snails from their resistant counterparts. In order to examine this apparent association between heat stress and snail susceptibility, we investigated the effect of temperature modulation in the resistant snail stock, BS-90. Here, we show that, incubated for up to 4 hrs at 32°C prior to infection, these resistant snails became susceptible to infection, i.e. shedding cercariae at 5 weeks post exposure (PE) while unstressed resistant snails, as expected, remained resistant. This suggests that susceptibility to infection by this resistant snail phenotype is temperature-sensitive (ts). Additionally, resistant snails treated with the Hsp 90 specific inhibitor, geldanamycin (GA) after heat stress, were no longer susceptible to infection, retaining their resistant phenotype. Consistently, susceptible snail phenotypes treated with 100 mM GA before parasite exposure also remained uninfected. These results provide direct evidence for the induction of stress genes (heat shock proteins; Hsp 70, Hsp 90 and the reverse transcriptase [RT] domain of the nimbus non-LTR retrotransposon) in B. glabrata susceptibility to S. mansoni infection and characterize the resistant BS-90 snails as a temperature-sensitive phenotype. This study of reversing snail susceptibility phenotypes to S. mansoni provides an opportunity to directly track molecular pathway(s) that underlie the B. glabrata snail's ability to either sustain or destroy the S. mansoni parasite.

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Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

Cited by 45 publications

References 65 publications

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Molluscan cells in culture: primary cell cultures and cell lines

Reversing the Resistance Phenotype of the Biomphalaria glabrata Snail Host Schistosoma mansoni Infection by Temperature Modulation

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