Larval Mouthparts and Feeding Mechanism of the Hessian Fly (Diptera: Cecidomyiidae)

Hatchett, J. H.; Kreitner, Gerald L.; Elzinga, Richard J.

doi:10.1093/aesa/83.6.1137

Cited by 57 publications

(37 citation statements)

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“…3). M. destructor larvae have sucking mouthparts and these proteinases may also be injected into host plant tissue for partial digestion before sucking the juice up (Haseman, 1930;Hatchett et al, 1990;Cohen, 1998). The function of diversified serine proteinase genes and differential expression of these genes in different developmental stages remains to be characterized.…”

Section: Article In Pressmentioning

confidence: 99%

Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [ (say)]

Zhu

Xiang

Maddur

et al. 2005

Insect Biochemistry and Molecular Biology

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Section: Article In Pressmentioning

confidence: 99%

Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [ (say)]

Zhu

Xiang

Maddur

et al. 2005

Insect Biochemistry and Molecular Biology

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“…The mandibles of first-instar Hessian fly larvae are specialized structures capable of injecting salivary fluids into cells at the surface of wheat leaf sheaths and meristems (Hatchett et al 1990). Five successive days of interaction with a single virulent firstinstar larva can lead to irreversible stunting of a susceptible seedling (Byers & Gallun 1971).…”

Section: Introductionmentioning

confidence: 99%

Modulation of defense-response gene expression in wheat during Hessian fly larval feeding

Sardesai

Subramanyam

Nemacheck

et al. 2005

Journal of Plant Interactions

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Expression profiles of ten genes commonly up-regulated during plant defense against microbial pathogens were compared temporally during compatible and incompatible interactions with first-instar Hessian fly larvae, in two wheat lines carrying different resistance genes. Quantitative real-time PCR revealed that while a lipoxygenase gene (WCI-2 ) was strongly upregulated during the incompatible interactions, genes encoding b-1,3 endoglucanase (GNS ) and an integral membrane protein (WIR1 ) were moderately responsive. Genes for thionin-like protein (WCI-3 ), PR-17-like protein (WCI-5 ), MAP kinase (WCK-1 ), phenylalanine ammonia-lyase (PAL ), pathogenesis-related protein-1 (PR-1 ), receptor-like kinase (LRK10 ) and heat shock protein 70 (HSP70 ) were minimally responsive. The application of signaling molecules, salicylic acid (SA), methyl jasmonate (MJ) and abscisic acid (ABA), to insect-free plants demonstrated association of these genes with specific defense-response pathways. SA-induced up-regulation of a gene related to lipoxygenases that are involved in jasmonic acid (JA)-biosynthesis is suggestive of positive cross-talk between SA-and JA-mediated signaling pathways. Data suggest that alternative mechanisms may be involved since few of these classical defense-response genes are significantly upregulated during incompatible interactions between wheat and Hessian fly.

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“…Hessian fly larvae hatch from the eggs and crawl down to the base of the plant (crown), where they feed on the abaxial surface of developing leaf sheaths (McColloch and Yuasa, 1917). The first-instar Hessian fly larvae have specialized mandibles that are capable of injecting salivary fluids into cells at the surface of wheat leaf sheaths and meristems (Hatchett et al, 1990). Resistance to Hessian fly attack is achieved through R gene-mediated resistance (Hatchett and Gallun, 1970).…”

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Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

et al. 2008

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We previously cloned and characterized a novel jacalin-like lectin gene from wheat (Triticum aestivum) plants that responds to infestation by Hessian fly (Mayetiola destructor) larvae, a major dipteran pest of this crop. The infested resistant plants accumulated higher levels of Hfr-1 (for Hessian fly-responsive gene 1) transcripts compared with uninfested or susceptible plants. Here, we characterize the soluble and active recombinant His 6 -HFR1 protein isolated from Escherichia coli. Functional characterization of the protein using hemagglutination assays revealed lectin activity. Glycan microarray-binding assays indicated strong affinity of His 6 -HFR1 to Mana1-6(Mana1-3)Man trisaccharide structures. Resistant wheat plants accumulated high levels of HFR1 at the larval feeding sites, as revealed by immunodetection, but the avirulent larvae were deterred from feeding and consumed only small amounts of the lectin. Behavioral studies revealed that avirulent Hessian fly larvae on resistant plants exhibited prolonged searching and writhing behaviors as they unsuccessfully attempted to establish feeding sites. During His 6 -HFR1 feeding bioassays, Drosophila melanogaster larvae experienced significant delays in growth and pupation, while percentage mortality increased with progressively higher concentrations of His 6 -HFR1 in the diet. Thus, HFR1 is an antinutrient to dipteran larvae and may play a significant role in deterring Hessian fly larvae from feeding on resistant wheat plants.

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Larval Mouthparts and Feeding Mechanism of the Hessian Fly (Diptera: Cecidomyiidae)

Cited by 57 publications

References 0 publications

Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [ (say)]

Cloning and characterization of chymotrypsin- and trypsin-like cDNAs from the gut of the Hessian fly [ (say)]

Modulation of defense-response gene expression in wheat during Hessian fly larval feeding

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

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