Larval stages of the bluefin tuna blood fluke Cardicola opisthorchis (Trematoda: Aporocotylidae) found from Terebella sp. (Polychaeta: Terebellidae)

Sugihara, Yukitaka; Yamada, Toshiyuki; Tamaki, Akio; Yamanishi, Ryohei; Kanai, Kazuaki

doi:10.1016/j.parint.2013.11.010

Cited by 46 publications

(22 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other farm infrastructure such as mooring lines and chains, walkways and buoys are cleaned at much lower frequencies, occasionally only following the grow-out phase. Therefore, biofouling accumulation on these structures can be considerable (Bloecher et al 2015) and may act as a reservoir for pathogens (Sugihara et al 2014;Shirakashi and Hirano 2015), likely exacerbating biofouling recruitment to cage nets (Bloecher et al 2015).…”

Section: Net Cleaning and Exchangementioning

confidence: 99%

Biofouling in marine aquaculture: a review of recent research and developments

et al. 2019

View full text Add to dashboard Cite

Biofouling in marine aquaculture is one of the main barriers to efficient and sustainable production. Owing to the growth of aquaculture globally, it is pertinent to update previous reviews to inform management and guide future research. Here, the authors highlight recent research and developments on the impacts, prevention and control of biofouling in shellfish, finfish and seaweed aquaculture, and the significant gaps that still exist in aquaculturalists' capacity to manage it. Antifouling methods are being explored and developed; these are centred on harnessing naturally occurring antifouling properties, culturing fouling-resistant genotypes, and improving farming strategies by adopting more sensitive and informative monitoring and modelling capabilities together with novel cleaning equipment. While no simple, quick-fix solutions to biofouling management in existing aquaculture industry situations have been developed, the expectation is that effective methods are likely to evolve as aquaculture develops into emerging culture scenarios, which will undoubtedly influence the path for future solutions. ARTICLE HISTORY

show abstract

Section: Net Cleaning and Exchangementioning

confidence: 99%

Biofouling in marine aquaculture: a review of recent research and developments

et al. 2019

View full text Add to dashboard Cite

show abstract

“…A total of 64terebellidpolychaetes, including 39 Terebella sp. were collected from ropes and floats attached to tuna cages at a PBTculture farm in Tsushima, Nagasaki Prefecture, Japan on February 15, 2016, the same site as Sugihara et al [7]collected infected polychaetes. Terebellidpolychaeteswere also sampledat a PBT culture farm in Kushimoto, Wakayama Prefecture, Japan on June 23, 2015 as a part of monthly samplings [6].…”

Section: Field Samplingmentioning

confidence: 99%

“…Each polychaete was flattened and examined for sporocysts in the body cavity by light microscopy in Tsushima as in Sugihara et al [8]or by stereomicroscopy in Kushimoto as in Shirakashi et al [6].When found infected, sporocystswere isolated in clean sea water and later used for morphological and molecular analyses. For sporocysts collected in Kushimoto, the 28S ribosomal DNA sequences were determined as 4 described in Shirakashi et al [6].For those collected in Tsushima, C. opisthorchis was confirmed by the sequences of the PCR products targeting the internal transcribedspacer 2 and 28S of the ribosomal DNA sequences, as described in Sugihara et al [7].The infected terebellids were fixed in 10% seawater formalin for traditional identification.…”

Section: Field Samplingmentioning

confidence: 99%

“…Sugihara et al [7]was the first to describe the larval stages of C. opisthorchis in the intermediate host.…”

Section: ) Remarksmentioning

confidence: 99%

“…Over the last few years, the intermediate hosts of all these blood flukes infecting PBT have been identified in Japan. Sugihara et al [7]demonstrated molecularly thatthe larval stages (cercaria and sporocyst) collected from Terebella sp. (Terebellidae) were those of C. opisthorchis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Developmental stages of fish blood flukes, Cardicola forsteri and Cardicola opisthorchis (Trematoda: Aporocotylidae), in their polychaete intermediate hosts collected at Pacific bluefin tuna culture sites in Japan

Ogawa

Shirakashi

Tani

et al. 2017

Parasitology International

Self Cite

View full text Add to dashboard Cite

Farming of Pacific bluefin tuna (PBT), Thunnus orientalis, is a rapidly growing industry in Japan. Aporocotylid blood flukes of the genus Cardicola comprising C. orientalis, C. opisthorchis and C. forsteri are parasites of economic importance for PBT farming. Recently, terebellid polychaetes have been identified as the intermediate hosts for all these parasites. We collected infected polychaetes, Terebella sp., the intermediate host of C. opisthorchis, from ropes and floats attached to tuna cages in Tsushima, Nagasaki Prefecture, Japan. Also, Neoamphitrite vigintipes (formerly as Amphitrite sp. sensu Shirakashi et al., 2016), the intermediate host of C. forsteri, were collected from culture cages in Kushimoto, Wakayama Prefecture, Japan. The terebellid intermediate hosts harbored the sporocysts and cercariae in their body cavity. Developmental stages of these blood flukes were molecularly identified using species specific PCR primers. In this paper, we describe the cercaria and sporocyst stages of C. opisthorchis and C. forsteri and compare their morphological characteristics among three Cardicola blood flukes infecting PBT. We also discuss phylogenetic relations of the six genera of the terebellid intermediate hosts (Artacama, Lanassa, Longicarpus, Terebella, Nicolea and Neoamphitrite) of blood flukes infecting marine fishes, based on their morphological characters.

show abstract