Larvicidal efficacy of Cryptomeria japonica leaf essential oils against Anopheles gambiae

Abstract

BackgroundAlternative insecticidal compounds with mortality effect against mosquito life cycle stages are currently needed. The compounds should be biodegradable and nontoxic to non-targeted insects. Plant based larvicides provide effective control of vector populations. This study explored Cryptomeria japonica leaf essential oil larvicidal potency against Anopheles gambiae sensu stricto.MethodsEssential oils (12.5 to 200 μg/mL) extracted from C. japonica leaves were evaluated against An. gambiae s.s. larvae in both the laboratory and semi field in 6 replicates for each dose. Larval mortality readings were taken at 12, 24, 48, and 72 h post treatment.ResultsC. japonica leaf essential oil yield was 17.06 ± 0.56 mL/kg and 1.60 ± 0.33% (w/w). GC-FID and GC-MS analyses revealed 22 constituents. Essential oil was more effective against An. gambiae s.s. larvae in the laboratory than in semi field trials. Mortality increased with increasing dosages (12.5 to 200 μg/mL) in the laboratory (31.75 to 100%) and semi field trials (17.75 to 99.5%), respectively. The LC50 value ranged from 5.55 to 63.92 μg/mL in the laboratory, and 8.22 to 134.84 μg/mL in semi field conditions, LC90 value ranged from 41.34 to 205.93 μg/mL in the laboratory and 50.92 to 213.11 μg/mL in semi field conditions.ConclusionThis study has demonstrated the potential of C. japonica leaf essential oil to cause mortality effects to An. gambiae s. s. larval populations, however, further studies need to be conducted under field conditions and also with individual active compounds of C. japonica essential oil.

Antibacterial Activity of Essential Oils and Their Isolated Constituents against Cariogenic Bacteria: A Systematic Review

“…Cryptomeria japonica (Cupressaceae) is an endemic and widely distributed coniferous plant in Japan, normally used for forestry, whose EO has been reported to have several pharmacological properties including larvicidal [ 51 ], antiulcer [ 52 ], antifungal [ 53 ] and antibacterial [ 20 ]. C. japonica EO is another example of how the complex mixture of chemical molecules plays a synergistic role in the antibacterial power of the EO over its isolated major compounds (sabinene, terpinen-4-ol, α-pinene and α-terpineol) [ 20 ].…”

Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

“…gambiae include larvae and/or pupae growth inhibitors [ 11 , 12 ], larvicides [ 13 ] and ovicides [ 14 ]. These phytochemicals were derived from various mahogany ( Meliaceae ) plant families [ 15 , 16 ], Japanese cedar ( Cryptomeria japonica ) [ 17 ], black pepper ( Piper nigrum ) [ 18 ], Indian wild pepper ( Vitex trifolia ) [ 19 ] and wild honeysuckle tree [ 20 ]. We recently assessed the effects of exposure of larval stages of An.…”

Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms

“…For that reason, require of ecofriendly green pesticide for mosquito control. So researcher find effective molecules from biological sources such as micro-organisms and plants contain considerable amount of metabolites/ essential oils which are promising alternative to synthetic insecticides [2][3][4][5][6].…”