Larvicidal, Ovicidal, Synergistic, and Repellent Activities of Sophora alopecuroides and Its Dominant Constituents Against Aedes albopictus

Shoukat, Rana Fartab; Shakeel, Muhammad; Rizvi, Syed Arif Hussain; Zafar, Junaid; Zhang, Yuxin; Freed, Shoaib; Xu, Xinyun; Jin, Fengliang

doi:10.3390/insects11040246

Cited by 30 publications

(25 citation statements)

References 51 publications

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“…M. anisopliae produces secondary metabolite destruxin, a compound capable of evading insect cellular and humoral immune responses, troubling the demographic parameters of the pest before its eventual death [ 73 , 74 , 75 , 76 ]. Disturbances in biological parameters of various insect pests have been reported when infected with entomopathogenic fungi [ 10 , 11 , 26 , 61 , 62 ], which strengthens our findings where a distress in biological cycles are reported. During the invasion of pathogens ( M. anisopliae ) the body temperature of insects rises to encounter the infection but this, in turn, affects the larval stadium [ 77 ] supporting the current findings where reduced larval duration was observed.…”

Section: Discussionsupporting

confidence: 91%

“…Several entomopathogenic fungi have been used to control insect pests from different orders such as Diptera [ 3 , 4 ], Hemiptera [ 5 ], Coleoptera [ 6 ], Homoptera [ 7 ], and Lepidoptera [ 8 ]. The excessive and injudicious use of synthetic insecticides has resulted in pest resurgence, environment degradation, resistance development while also causing harmful effects to human health [ 9 , 10 , 11 ]. Such detrimental effects of chemical insecticides stressed the need to develop alternative control strategies.…”

Section: Introductionmentioning

confidence: 99%

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Metarhizium Anisopliae Challenges Immunity and Demography of Plutella xylostella

Zafar

Shoukat

Zhang

et al. 2020

Insects

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Entomopathogenic fungi are naturally existing microbes, that can serve as a key regulator of insect pests in integrated pest management strategies. Besides having no hazardous effects on the environment, these entomopathogens are alternatives to synthetic insecticides that can control notorious insect-like Plutella xylostella, a destructive pest of cruciferous crops. Three different species of entomopathogenic fungi were evaluated before the selection (high larval mortality and least LC50) of Metarhizum anisopliae. The study was designed to investigate the mortality, development, and immune responses of P. xylostella when challenged with M. anisopliae, a naturally existing soil-borne entomopathogenic fungus. M. anisopliae resulted in high pest mortality by killing 93% of larvae. However, no statistically significant effect on hemocyte concentration was observed. The activity of enzymes (Phenoloxidase and Superoxide dismutase) and immune genes (Defensin, Spaetzle, Cecropin, Lysozyme, and Hemolin) did vary at different time points (24, 48, 72 and 96 h) after exposure to M. anisopliae. Disturbance in the biological cycles of P. xylostella was also detected, significantly shorter adult life span (8.11:6.87, M:F) and reduced fecundity (101 eggs/female) were observed along with disturbed larval and pupal duration. Results suggest that M. anisopliae can efficiently hinder the P. xylostella defense and developmental system, resulting in mortality and disturbed demography.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Metarhizium Anisopliae Challenges Immunity and Demography of Plutella xylostella

Zafar

Shoukat

Zhang

et al. 2020

Insects

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“…The ovicidal activity was evaluated by the protocol of Shoukat et al, (2020). Thirty freshly laid eggs of A. aegypti, A. stephensi and C. quinquefasciatus were treated with synthesized AgNPs at 5, 10, 15, 20 and 25 ppm concentrations.…”

Section: Ovicidal Activitymentioning

confidence: 99%

A Novel Entomopathogenic Actinobacteria Mediated Silver Nanoparticles Characterization and Their Insecticidal, Antibacterial and Cytotoxic Activities

Raguvaran

Kalpana

Manimegalai

et al. 2021

Preprint

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Nanomaterials were highly inspired in the field of nanotechnology especially bio synthesized silver nanoparticles (AgNPs) have effectively attracted worldwide that helps to improve in the field of medical science and human health. Actinabacteria mediated nanoparticle were widely used to control insect pests, treatment of antibiotic resistant bacteria, cancer and other diseases due to their potential pharmacological properties compared with other chemical drugs. The present investigation an entomopathogenic actinobacteria Actinokineospora fastidiosa was isolated and synthesis of AgNPs and tested their mosquito larvicidal, antibacterial and anticancer activity. Moreover, the outcome of biosynthesized AgNPs was characterized by UV-visible spectroscopy, X-ray Diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), Transmission electron microscope (TEM), scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDX) and zeta potential analysis. The characterized AgNPs showed a potent larvicidal activity against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus the obtained LC50 values is 9.86 ppm, 8.50 ppm and 8.29 ppm respectively. However, at 25 ppm concentration, AgNPs showed highest ovicidal activity (100 %, 100 % and 97.77 % against A. aegypti, A. stephensi and C. quinquefasciatus respectively) and oviposition deterrence activity was 100 %, 100 % and 98.66 % were achieved against A. aegypti, A. stephensi and C. quinquefasciatus respectively. The enzymes of α, β esterase and Glutathione-S-transferase level were increased due to detoxification process against AgNPs. The histopathological results of AgNPs showed series damage in epithelial cells and brush border cell of mosquito larval gut. In addition, actinobacterial mediated AgNPs was tested against different human pathogenic bacteria. The obtained results showed biosynthesized AgNPs revealed remarkable antibacterial activity against all tested pathogens. The lowest mortality was observed on non-target organisms of Artemia salina at highest concentration of AgNPs. Finally, in vitro toxicity study showed that biosynthesized AgNPs potential anticancer activity and induced reactive oxygen species (ROS) production in human cervical cancer (HeLa) cell line at an inhibitory concentration (IC50) range 42.37 μg/mL. Therefore, it can be concluded that biosynthesized AgNPs using a novel stains Actinokineospora fastidiosa potentially influenced the vector mosquito and bacterial diseases management and pharmacological applications in eco-friendly manner.

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“…aegypti [17] while the larvicidal, ovicidal, and repellent activity of Sophora alopecuroides and synergistic activity of its dominant constituents have been documented against Ae. albopictus [18].…”

Section: Introductionmentioning

confidence: 99%

Ovicidal Activity of 2-Hydroxy-4-Methoxybenzaldehyde, Derivatives and Structural Analogues on Anopheles gambiae eggs

Omolo

Andati

Ndiege

2021

Preprint

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Background Effective remedies for disrupting Anopheles gambiae metamorphosis at the egg stage are crucial in suppression of the malaria vector populations that result in the reduction of disease burden. 2-Hydroxy-4-methoxybenzaldehyde (the major component of Mondia whytei roots), its derivatives, structural analogues and their blends were evaluated against the eggs of An. gambiae in the search for ovicidal compounds with potential use in mosquito control programs. Methods Mature roots were harvested from Mondia whytei plants grown in the Center for African Medicinal & Nutritional Flora and Fauna (CAMNFF) herbal medicinal garden and cleaned with distilled water. 2-Hydroxy-4-methoxybenzaldehyde ( 1 ) was isolated by steam distillation of the chopped roots. The selected derivatives and/or analogues were prepared using established chemical procedures and their structures confirmed by NMR spectroscopy and ESI-MS. Ovicidal activity of the pure compounds, derivatives, structural analogues and/or formulated blends was tested at 1, 10, 25 and 50 ppm on An. gambiae eggs. . Results Eleven mono-substituted ( 3-7 ), di-substituted ( 8-10 ), tri-substituted ( 1-2 ) aromatic compounds were assayed for ovicidal activity against Anopheles gambiae eggs singly or as blends. Benzaldehyde ( 4 ) and 4-methoxybenzaldehyde ( 9 ) were further converted into 2-hydroxy-1, 2-diphenylethanone ( 11 ), 1, 5-diphenylpenta-1, 4-diene-3-one ( 12 ) and 1, 5- bis (4-methoxyphenyl) penta-1, 4-diene-3-one ( 13 ) and evaluated for ovicidal activity individually or as blends. Of the thirteen compounds evaluated individually, 2-hydroxy-4-methoxybenzaldehyde ( 1 ) exhibited the highest ovicidal activity at LC 50 0.7075 ppm while anisole had the lowest activity at LC 50 40.342 ppm. The derivatives exhibited moderate activity: 2-hydroxy-1, 2-diphenylethanone (LC 50 10.599 ppm), 1, 5-diphenylpenta-1, 4-diene-3-one (LC 50 9.019 ppm) and 1, 5-bis (4-methoxyphenyl) penta-1, 4-diene-3-one (LC 50 15.642 ppm). The blends exhibited intriguingly high ovicidal efficacy with the mixture of benzaldehyde and phenol showing the highest (LC 50 0.332 ppm) while phenol and anisole exhibited the lowest activity (LC 50 9.9909 ppm). Conclusion From the activity of the blends, it is evident that anisole is antagonistic to the efficacy of phenol and benzaldehyde. It is also apparent that aldehyde and hydroxyl groups, when directly attached to the phenyl ring, provide the critical structural characteristics that contribute to the ovicidal activity of the aromatic compounds.

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Larvicidal, Ovicidal, Synergistic, and Repellent Activities of Sophora alopecuroides and Its Dominant Constituents Against Aedes albopictus

Cited by 30 publications

References 51 publications

Metarhizium Anisopliae Challenges Immunity and Demography of Plutella xylostella

Metarhizium Anisopliae Challenges Immunity and Demography of Plutella xylostella

A Novel Entomopathogenic Actinobacteria Mediated Silver Nanoparticles Characterization and Their Insecticidal, Antibacterial and Cytotoxic Activities

Ovicidal Activity of 2-Hydroxy-4-Methoxybenzaldehyde, Derivatives and Structural Analogues on Anopheles gambiae eggs

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