Laryngeal abductor muscle reinnervation in a pig model

Birchall, Martin A.; Idowu, Bernadine; Murison, PJ; Jones, Alan J.; Burt, Rachel; Ayling, Sarah; Stokes, Chris R.; Pope, Laysan; Terenghi, Giorgio

doi:10.1080/00016480410022507

Cited by 14 publications

(23 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No attempt was made to repair nerves since the primary aim was to study/perfect the transplant surgery and no functional recovery would occur by 1 week in any case. We have described nerve repair elsewhere [18, 26]. Mucosal biopsies were taken on reperfusion, which marked the end of cold ischaemia.…”

Section: Methodsmentioning

confidence: 99%

Laryngeal transplantation in minipigs: vascular, myologic and functional outcomes

Birchall

Kingham

Murison

et al. 2010

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

There is no effective way of replacing all the functions of the larynx in those requiring laryngectomy. Regenerative medicine offers promise, but cannot presently deliver implants with functioning neuromuscular units. A single well-documented laryngeal transplant in man was a qualified success, but more information is required before clinical trials may be proposed. We studied the early response of the larynx to laryngeal transplantation between 17 pairs of NIH minipigs full matched at the MHC2 locus. Following iterative technical improvements, pigs had good swallowing and a patent airway at 1 week. No significant changes in mucosal blood flux were observed compared with pre-operative measurements. Changes in muscle morphology and fibre phenotype were observed in transplant muscles retrieved after 7 days: the levels of fast and slow myosin heavy chain (MyHC) protein were reduced and embryonic MyHC was up regulated consistent with denervation induced atrophy. At 1 week laryngeal transplantation can result in good swallowing, and is not associated with clinical evidence of ischemia-reperfusion injury in MHC-matched pigs.

show abstract

Section: Methodsmentioning

confidence: 99%

Laryngeal transplantation in minipigs: vascular, myologic and functional outcomes

Birchall

Kingham

Murison

et al. 2010

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

show abstract

“…The results from other studies on the immunological architecture of mucosal tissues, together with the present results in the larynx, suggest that this is attributable to the high prevalence of antigen-presenting cells (MHC class II + ) throughout mucosal tissues. This is of increasing importance as laryngeal transplantation becomes an increasingly viable therapeutic prospect due, in part, to the harnessing of modern neuroscience to the problem of functional laryngeal reinnervation [15,16]. Thus, when laryngeal transplantation is performed, the presence of these cells is likely to affect adversely survival of an allograft, confirming the need for studies of effective immunosuppression or specific immunomanipulation targeted to the local class II-CD4 + cell axis.…”

Section: Discussionmentioning

confidence: 97%

“…One successful laryngeal transplant has been performed on a human subject [13], and the patient continues to do well in terms of speech and swallowing 7 years later ( [14]; Strome 2005, personal communication). Limited re-innervation did occur in this patient, and the problem of functional restoration is under study in several human and animal models, including the pig [15][16][17]. However, several questions regarding long-term survival and immunosuppression need to be answered before routine clinical use can be advocated.…”

Section: Introductionmentioning

confidence: 99%

The larynx as an immunological organ: immunological architecture in the pig as a large animal model

Barker

Haverson

Stokes

et al. 2005

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryThe larynx is a mucosal organ positioned at the divergence of the respiratory and digestive tracts. It is exposed to a wide variety of environmental components, including foreign antigens, tobacco smoke, laryngopharyngeal reflux and pollutants. The mucosal immune system generates either active immune responses or tolerance, depending on the nature of the antigen and we hypothesize that the larynx is important organ for immunological decisionmaking in the airway. Because the pig is an ideal large animal model in which to explore laryngological research questions, such as those relating to laryngeal transplantation, we investigated the normal mucosal immunology of the porcine larynx. Pig larynges and tracheae were processed and prepared for bright-field microscopy and quantitative, multiple-colour immunofluorescence histology using pig-specific monoclonal antibodies. There was an abundance of immunologically active cells within the mucosa of the larynx and trachea of both the newborn and adult animal. Specifically, major histocompatibility complex class II (MHC class II

show abstract

“…Current surgical procedures cannot restore true laryngeal motion. Thus, our group has been examining novel methods to repair the injured RLN using techniques developed for the treatment of other peripheral nerve injuries (Birchall et al. 2004; Kingham et al.…”

Section: The Larynx and Recurrent Laryngeal Nerve Injurymentioning

confidence: 99%

“…We have built on our previous work with the use of PHB nerve conduits for peripheral nerve repair. Using a pig model we performed unilateral RLN transection followed by anastomosis of the phrenic and RLN repaired with PHB mats (Birchall et al. 2004; Kingham et al.…”

Section: Neurotrophic Factors and Nerve Conduits For Rln Repair And Lmentioning

confidence: 99%

Bioengineered nerve regeneration and muscle reinnervation

2006

Self Cite

View full text Add to dashboard Cite

The peripheral nervous system has the intrinsic capacity to regenerate but the reinnervation of muscles is often suboptimal and results in limited recovery of function. Injuries to nerves that innervate complex organs such as the larynx are particularly difficult to treat. The many functions of the larynx have evolved through the intricate neural regulation of highly specialized laryngeal muscles. In this review, we examine the responses of nerves and muscles to injury, focusing on changes in the expression of neurotrophic factors, and highlight differences between the skeletal limb and laryngeal muscle systems. We also describe how artificial nerve conduits have become a useful tool for delivery of neurotrophic factors as therapeutic agents to promote peripheral nerve repair and might eventually be useful in the treatment of laryngeal nerve injury.

show abstract

Laryngeal abductor muscle reinnervation in a pig model

Cited by 14 publications

References 10 publications

Laryngeal transplantation in minipigs: vascular, myologic and functional outcomes

Laryngeal transplantation in minipigs: vascular, myologic and functional outcomes

The larynx as an immunological organ: immunological architecture in the pig as a large animal model

Bioengineered nerve regeneration and muscle reinnervation

Contact Info

Product

Resources

About