Laser ablation electrospray ionization mass spectrometry imaging as a new tool for accessing patulin diffusion in mold-infected fruits

Lima, Gesiane da Silva; Santos, Glauber Eduardo de Oliveira; Ramalho, Ruver Rodrigues Feitosa; Aguiar, Deborah Victória Alves de; Roque, Jussara V.; Maciel, Lanaia Ítala Louzeiro; Simas, Rosineide C.; Vaz, Boniek Gontijo

doi:10.1016/j.foodchem.2021.131490

Cited by 24 publications

(26 citation statements)

References 39 publications

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“…This process creates a cloud of analytes, which are then ionized with an electrospray [76]. So far, LAESI-MS couplings have been applied, for example, for the nontargeted differentiation of meat species and for the detection of contaminants in fruits [77][78][79]. Instead of nanosecond lasers, picosecond infrared lasers (PIRL) can also be applied to achieve a more efficient vaporization process [80].…”

Section: Ionization Sourcesmentioning

confidence: 99%

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Creydt¹,

Fischer²

2022

Electrophoresis

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The analytical requirements for food testing have increased significantly in recent years. On the one hand, because food fraud is becoming an ever-greater challenge worldwide, and on the other hand because food safety is often difficult to monitor due to the far-reaching trade chains. In addition, the expectations of consumers on the quality of food have increased, and they are demanding extensive information. Cutting-edge analytical methods are required to meet these demands. In this context, non-targeted metabolomics strategies using mass and nuclear magnetic resonance spectrometers (mass spectrometry [MS]) have proven to be very suitable. MS-based approaches are of particular importance as they provide a comparatively high analytical coverage of the metabolome.Accordingly, the efficiency to address even challenging issues is high. A variety of hardware developments, which are explained in this review, have contributed to these advances. In addition, the potential of future developments is highlighted, some of which are currently not yet commercially available or only used to a comparatively small extent but are expected to gain in importance in the coming years.

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Section: Ionization Sourcesmentioning

confidence: 99%

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Creydt¹,

Fischer²

2022

Electrophoresis

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“…In contrast to MALDESI, LAESI requires little to no sample preparation, which propels the technique towards rapid analysis of large sample sets [83][84][85]. However, recent IR-MALDESI studies using only the samples' water or solvent as matrix have shown that extremely high throughput can also be achieved by MALDESI as shown by the analysis of isocitrate dehydrogenase 1 (IDH1) kinetics assays [86].…”

Section: Laesimentioning

confidence: 99%

“…First described by Nemes and Vertes in 2007, LAESI utilises mid‐IR laser ablation orthogonal to an ESI source for the direct analysis of samples with a variety of surface features, as long as the samples have a sufficiently high water content [82]. In contrast to MALDESI, LAESI requires little to no sample preparation, which propels the technique towards rapid analysis of large sample sets [83–85]. However, recent IR‐MALDESI studies using only the samples’ water or solvent as matrix have shown that extremely high throughput can also be achieved by MALDESI as shown by the analysis of isocitrate dehydrogenase 1 (IDH1) kinetics assays [86].…”

Section: Spray‐based Ionisation Techniquesmentioning

confidence: 99%

Advances in ionisation techniques for mass spectrometry‐based omics research

Challen

Cramer

2022

Proteomics

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Omics analysis by mass spectrometry (MS) is a vast field, with proteomics, metabolomics and lipidomics dominating recent research by exploiting biological MS ionisation techniques. Traditional MS ionisation techniques such as electrospray ionisation have limitations in analyte-specific sensitivity, modes of sampling and throughput, leading to many researchers investigating new ionisation methods for omics research. In this review, we examine the current landscape of these new ionisation techniques, divided into the three groups of (electro)spray-based, laser-based and other miscellaneous ionisation techniques. Due to the wide range of new developments, this review can only provide a starting point for further reading on each ionisation technique, as each have unique benefits, often for specialised applications, which promise beneficial results for different areas in the omics world.

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“…Then, an electrospray positioned above the sample ionizes the desorbed analytes, which go toward the mass spectrometer inlet to be analyzed by MS . LAESI usually uses an IR laser beam tuned to 2.94 μm to overlap with the O–H vibrational absorption of water molecules, which makes this method very useful for monitoring the spatial distribution of analytes in water-rich biological samples. − However, the IR laser beam can be adjusted to a different wavelength to meet different research goals. For example, at 3.4 μm, the IR laser beam can overlap with the C–H stretch absorption of nonpolar compounds and may assist in analyzing hydrocarbon-rich samples, such as reservoir rocks.…”

Section: Introductionmentioning

confidence: 99%

“…The LAESI system is illustrated in Figure S2 and has been described previously. 31 It consists of a pulsed IR optical parametric oscillator (IR Opolette, Opotek, Carslband) combined with the Omni Spray Ion Source 2D and the Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap described in the DESI analysis subsection. The rocks were mounted on the 2D moving stage and were irradiated by a 3.4 μm IR laser beam at a 90°angle.…”

Section: ■ Introductionmentioning

confidence: 99%

Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

et al. 2022

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Here, we present a new application of desorption electrospray ionization (DESI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging to assess the spatial location of organic compounds, both polar and nonpolar, directly from rock surfaces. Three carbonaceous rocks collected from an aquatic environment and a berea sandstone subjected to a small-scale oil recovery experiment were analyzed by DESI and LAESI. No rock pretreatment was required before DESI and LAESI analyses. DESI detected and spatially mapped several fatty acids and a disaccharide on the surfaces of carbonaceous rocks, and various nitrogenated and oxygenated compounds on the surfaces of berea sandstone. In contrast, LAESI using a 3.4 μm infrared laser beam was able to detect and map hydrocarbons on the surfaces of all rock samples. Both techniques can be combined to analyze polar and nonpolar compounds. DESI can be used first to detect polar compounds, as it does not destroy the rock surface, and LAESI can then be used to analyze nonpolar analytes, as it destroys a layer of the sample surface. Both techniques have the potential to be used in several scientific areas involving rocks and minerals, such as in the analysis of industry-derived contaminants in aquatic sediments or in small-scale rock–fluid interaction experiments.

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Laser ablation electrospray ionization mass spectrometry imaging as a new tool for accessing patulin diffusion in mold-infected fruits

Cited by 24 publications

References 39 publications

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Advances in ionisation techniques for mass spectrometry‐based omics research

Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

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