Laser-Assisted End-to-End Vascular Anastomosis of Elastin Heterograft to Carotid Artery with an Albumin Stent in Vivo

Abstract

Laser-assisted vascular anastomosis of elastin heterograft to medium size vessel is possible. Albumin stent played an important role in strength of the anastomosis. Chronic studies are warranted to determine long-term patency and histology of the laser-assisted vascular anastomosis.

Laser‐assisted end‐to‐end bioweld® anastomosis in an ovine model

“…By employing the ''fold and bond'' technique, we have been able to reduce the amount of laser energy required to form a stable anastomosis. This was achieved by delivering approximately 120 J of laser energy per completed anastomosis, compared to the 212 J required to achieve the same endstate in a similar sized porcine arterial model [22] at an output power approximately 30% of that described as optimal by Ren et al [23]. As energy is delivered to the target tissue, the ICG dye within the BioWeld 1 solder causes preferential absorption of the laser.…”

Improving the strength of sutureless laser-assisted vessel repair using preloaded longitudinal compression on tissue edge

“…Recent research has evaluated the ability of chemical materials, such as glues and/or dyes, to increase laser absorption and thus improve tissue welding strength . Most in vivo studies have used stay sutures , to approximate both edges of vessel segments and to increase tension.…”

“…Pushing the sample towards the coapted tissue end prior to irradiation may avoid tissue separation during laser irradiation. A review paper published in 2004 identified 37 relevant studies of LAVA/LAVR , and we found additional 31 relevant studies published mainly after 2004 . Of these 68 studies, 26 evaluated LAVA/LAVR without stay sutures .…”

Temporal heating profile influence on the immediate bond strength following laser tissue soldering

“…Most researchers do not incorporate temperature control in their LTB system. They usually apply a constant laser power and the tissue reaches some steady-state temperature (which is unknown, and can only be estimated in some of the cases) [15][16][17][18]. The few researchers who did use temperature control in their system heated abruptly the bonded surface to a desired temperature, T set .…”

“…The temperature rise resembled a ''step'' function, whose rise time was roughly 3-4 seconds. Once the settemperature, T set , was reached, the heated spot was either kept at that temperature for some predetermined dwell time, or was slowly translated along the cut at a constant rate [14][15][16][17][18][19][20][21][22][23][24].…”