Laser computed-tomography microscope

Tomographic diffractive microscopy: Towards high-resolution 3-D real-time data acquisition, image reconstruction and display of unlabeled samples

“…-TDM and its variants give access to the index of refraction distribution within the observed specimen, at the microscopic scale, and the interest of this non-labeling imaging modality has been widely demonstrated for biological investigations as well as for studying artificial structures [15][16][17][18][19][20][21][22][23][24][25][26][27][28]32,37,38,[41][42][43][44][45]. Note that the index of refraction is a complex quantity, related to the refraction and to the absorption.…”

“…While the precision of measurement of interferometric approaches is unsurpassed [12,13], the resolution when reconstructing 3-D transparent specimens is plagued by the small quantity of information, which is recorded, because of the use of only a single illumination direction [14]. In order to increase the recorded quantity of information, a rotation of the specimen [15][16][17][18][19][20][21][22], or a variation of the illumination wave inclination [23][24][25][26][27][28] can be used, and the set of recorded interferograms represents a diffractive tomographic acquisition [29,30]. This allows for a numerical 3-D reconstruction of the observed specimen.…”

Computational confocal tomography for simultaneous reconstruction of objects, occlusions, and aberrations

“…Of course, imaging the sample attenuation coefficient is itself possible by measurement of intensity instead of phase. Katawa et al [9] produced images of spirogyra in a system that essentially achieves rotation of the sample within a collimated beam. This fairly direct application of computed tomography to optics is the most common approach.…”

Tomographic diffractive microscopy with agile illuminations for imaging targets in a noisy background

“…Indeed, the DORT illuminations dimmed the influence of the clutter and allowed the restriction of the investigation domain to small regions surrounding the targets [6]. In this work, we adapt this procedure to optical microscopy and show experimentally its interest for imaging objects of various sizes in a noisy background.Presently, the most powerful optical microscopy technique yielding three-dimensional images of marker-free samples is tomographic diffraction microscopy (TDM) also known as synthetic aperture microscopy or diffraction phase microscopy [7][8][9][10][11][12][13][14][15]. In this approach, the sample is illuminated with a collimated beam under various incident angles and polarization states, and its scattered field (phase and amplitude) is recorded for a large number of observation directions within the numerical aperture (NA) of the microscope objective.…”