Laser Desorption Ionization Mass Spectrometry on Silicon Nanowell Arrays

Gülbakan, Basri; Park, Dooho; Kang, Myungchan; Keçeci, Kaan; Martin, Charles R.; Powell, David H.; Wang, Tan

doi:10.1021/ac101149b

Cited by 23 publications

(20 citation statements)

References 33 publications

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“…An increased surface area can enhance LDI signals [1,2,7,8,10], but comparisons among NPG surfaces showed differences between surface area and LDI efficiency. Although the RSA is larger by the order of NPG #59#49#39#29#1 (refer to Figure 1S, Supporting Information), the LDI signal from NPG #1 was larger than those from NPG #2 and #3.…”

Section: Evaluation and Comparison Of Npg Surfaces For Ldi-tof-msmentioning

confidence: 97%

“…Particles or surfaces with nanostructures have been combined with LDI to improve ionization efficiency and reduce matrix interference [1][2][3][4][5][6][7][8][9][10][11][12][13]. Although many studies have employed nanostructures to improve LDI efficiency and selectivity, relatively few reports have examined how the nanostructure is related to LDI efficiency [9].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Nanoporous Gold with Controlled Surface Structures for Laser Desorption Ionization (LDI) Analysis: Surface Area Versus LDI Signal Intensity

Jin

Choi

Kim

et al. 2012

J. Am. Soc. Mass Spectrom.

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The structural effect of a nanoporous gold (NPG) surface on the signal intensities of laser desorption ionization-mass spectrometry (LDI-MS) were investigated using NPG surfaces with controlled structures. The relationship between surface area and LDI efficiency was compared and evaluated. Comparisons between bare flat gold and NPG surfaces show that nanostructures increased LDI efficiency. We also found that the LDI signal decreased with increasing depth of nanoporous layers, thus increasing the surface area. This result agrees with a previous report (Shin J. A. et al., J. Am. Soc. Mass Spectrom. 2010, 21, 989) in which the LDI efficiency of small molecules decreased for ZnO wires with longer lengths. This observation was explained by the penetration and deposition of samples into locations inaccessible to photons because of structural screening. The LDI-MS analysis of oils with NPG surfaces (but without matrix) showed the same trend whereby the NPG with about a 200 nm depth of porous area showed the highest sensitivity. This study clearly shows that the active surface area for solution chemistry can differ from LDI-MS and that NPGs can function as a substrate for LDI oil analysis.

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Section: Evaluation and Comparison Of Npg Surfaces For Ldi-tof-msmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Evaluation of Nanoporous Gold with Controlled Surface Structures for Laser Desorption Ionization (LDI) Analysis: Surface Area Versus LDI Signal Intensity

Jin

Choi

Kim

et al. 2012

J. Am. Soc. Mass Spectrom.

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show abstract

“…It has also been applied in mass spectrometric imaging studies (Yanes et al, 2009). In a recent study by Gulbakan et al (2010), anodic nanoporous alumina was used efficiently to make well-ordered nanowell array surfaces. In this study, small molecules and peptides (adenosine, bradykinin, tripeptide) were analyzed by changing the pore depth (10-50 nanometers).…”

Section: Nanostructured Surfacesmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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“…Sample preparation Similar to MALDI MS practice, the samples for SALDI MS can be prepared in several ways: The analyte can be mixed with NPs and a suitable buffer prior deposition (dry droplet method), 47 loaded directly onto a plate coated with single-or multi-layer NPs or having a nanostructured surface (matrix-first method) 48 or deposited first and subsequently covered by NPs (sample-first method).…”

Section: 44-46mentioning

confidence: 99%

Analysis of Biomolecules through Surface‐Assisted Laser, Desorption/Ionization Mass Spectrometry Employing Nanomaterials

Chen

Tomalová

Preisler

2011

J Chinese Chemical Soc

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Nanomaterials, primarily nanoparticles (NPs), can serve as an alternative matrix for the analysis of various biomolecules through surface-assisted laser desorption/ionization mass spectrometry (SALDI MS). SALDI MS has been developed to overcome poor reproducibility and high background in the lowmass region commonly occurring in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Various nanomaterials, unmodified or functionalized with recognition ligands, can have a strong affinity toward certain analytes and thus are applicable for their concentration and enrichment from complex biological matrices. In mass spectrometry imaging (MSI), the use of NPs instead of the conventional matrices can improve the spatial resolution up to the cellular level. In this review, the nature of NPs, the methods of sample preparation and approaches for quantitation of biomolecules through SALDI MS are discussed. Practical applications and limitations of SALDI MS employing NPs for separate samples and MSI are mentioned. With regard to the nature of MSI analysis, the use of nanostructured surfaces for MSI is also reflected in this review.

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Laser Desorption Ionization Mass Spectrometry on Silicon Nanowell Arrays

Cited by 23 publications

References 33 publications

Evaluation of Nanoporous Gold with Controlled Surface Structures for Laser Desorption Ionization (LDI) Analysis: Surface Area Versus LDI Signal Intensity

Evaluation of Nanoporous Gold with Controlled Surface Structures for Laser Desorption Ionization (LDI) Analysis: Surface Area Versus LDI Signal Intensity

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Analysis of Biomolecules through Surface‐Assisted Laser, Desorption/Ionization Mass Spectrometry Employing Nanomaterials

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