Laser Doppler Microscopy: Especially as a Method for Studying Brownian Motion and Flow in the Sieve Tubes of Plants

Johnson, Richard P.

doi:10.1007/978-1-4684-4487-2_9

Cited by 2 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these studies laser beams of relatively large diameter (100 pm) were used to detect cytoplasmic steaming in coenocytic organisms. Only rarely have the results from light scattering experiments on living systems been identified with motions of specific structures (Johnson, 1983) or macromolecules (Carlson, 1983;Fan et al, 1983). Here we describe, for the first time, the analysis of diffusional motions within a small, precisely defined volume of living cytoplasm and the identification of the cell structures most likely to be undergoing these motions.…”

Section: Introductionmentioning

confidence: 99%

Diffusive motions in living cytoplasm probed by laser Doppler microscopy

Steer¹,

Picton²,

Earnshaw³

1984

Journal of Microscopy

View full text Add to dashboard Cite

We have examined cytoplasmic motions in growing pollen tube tips using laser Doppler microscopy, a non-invasive and non-destructive technique. The observed correlation functions were consistent with the existence of diffusive motions in the tip cytoplasm. Further analysis of the data revealed the existence of at least two major and a third, minor, diffusing species in the cytoplasm. Information from quantitative electron microscopy and from published literature values of cytoplasmic viscosity was used to tentatively identify the organelles contributing to the observed diffusive motions. It is concluded that motions of mitochondria and secretory vesicles dominate the observed correlation functions with a minor component detected from either ribosomes or the microfilament network. Sources of variation in the observed data are discussed, as are the physical limitations that prevent a more positive analysis of the present data.

show abstract

Section: Introductionmentioning

confidence: 99%