Laser Heating of Gold Nanospheres Functionalized with Octreotide: <i>In Vitro</i> Effect on HeLa Cell Viability

Mendoza-Nava, Héctor; Ferro-Flores, Guillermina; Ocampo‐García, Blanca; Serment-Guerrero, Jorge; Santos-Cuevas, Clara; Jiménez-Mancilla, Nallely; Luna-Gutiérrez, Myrna; Camacho-López, Miguel A.

doi:10.1089/pho.2012.3320

Cited by 25 publications

(23 citation statements)

References 25 publications

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“…14,15,17,[38][39][40][41][42][43] Almost all have not included a negative relevant control cell line to ensure specificity. [14][15][16][17]38,[43][44][45] Therefore, by directing energy provided from laser sources to α-PSMA-conjugated PEG-functionalized MWCNTs placed in extreme proximity to the surface of tumor cells, we have demonstrated the capacity of this platform to exclusively damage and/or destroy targeted cells upon treatment without significant bulk heating. To achieve over 80% of targeted cell ablation (approximately 240,000 cells out of 300,000 cells), only 0.33 µg of functionalized MWCNTs, that would approximately equate to 100,000 particles per cell with cells expressing approximately 180,000 PSMA surface antigens, 46 was needed to be exposed by a 2.7-W laser for 30 s (Figure 4(a)) without a significant increase in medium bulk temperature.…”

Section: Discussionmentioning

confidence: 99%

Prostate-specific membrane antigen–directed nanoparticle targeting for extreme nearfield ablation of prostate cancer cells

et al. 2017

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Almost all biological therapeutic interventions cannot overcome neoplastic heterogeneity. Physical ablation therapy is immune to tumor heterogeneity, but nearby tissue damage is the limiting factor in delivering lethal doses. Multi-walled carbon nanotubes offer a number of unique properties: chemical stability, photonic properties including efficient light absorption, thermal conductivity, and extensive surface area availability for covalent chemical ligation. When combined together with a targeting moiety such as an antibody or small molecule, one can deliver highly localized temperature increases and cause extensive cellular damage. We have functionalized multi-walled carbon nanotubes by conjugating an antibody against prostate-specific membrane antigen. In our in vitro studies using prostate-specific membrane antigenpositive LNCaP prostate cancer cells, we have effectively demonstrated cell ablation of >80% with a single 30-s exposure to a 2.7-W, 532-nm laser for the first time without bulk heating. We also confirmed the specificity and selectivity of prostate-specific membrane antigen targeting by assessing prostate-specific membrane antigen-null PC3 cell lines under the same conditions (<10% cell ablation). This suggests that we can achieve an extreme nearfield cell ablation effect, thus restricting potential tissue damage when transferred to in vivo clinical applications. Developing this new platform will introduce novel approaches toward current therapeutic modalities and will usher in a new age of effective cancer treatment squarely addressing tumoral heterogeneity.

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Section: Discussionmentioning

confidence: 99%

Prostate-specific membrane antigen–directed nanoparticle targeting for extreme nearfield ablation of prostate cancer cells

et al. 2017

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“…Several authors have mentioned that the local temperature near the AuNP is increased up to approximately 700-800 ∘ , which is lower than the fusion temperature of gold (1063 ∘ C) [7][8][9][10][11]. Therefore, a temperature increase of the order of 10 ∘ C at macroscopic levels could be expected.…”

Section: Laser Irradiationmentioning

confidence: 99%

“…The density power DP was kept constant at 0.65 W/cm 2 for each irradiation time point (6.5, 3.5, and 1.5 min) which corresponds to the 5, 10, and 15 Hz frequencies, respectively [8,24].…”

Section: Laser Irradiation Experimentsmentioning

confidence: 99%

“…The photothermal ablation process generated by AuNPs is due to the optical absorption phenomenon present when AuNPs are exposed to light that induces a collective oscillation of the free electrons (surface plasmon resonance, SPR) [1,[4][5][6]. The temperature around the AuNPs reaches 800 ∘ C and causes irreversible thermal destruction to the tissue [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Study of the Optical Properties of Functionalized Gold Nanoparticles in Different Tissues and Their Correlation with the Temperature Increase

Carrillo-Cazares¹,

Jiménez-Mancilla²,

Luna-Gutiérrez³

et al. 2017

Journal of Nanomaterials

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Mie theory explains the interaction of light with a gold nanoparticle (AuNP) through the absorption ( abs ), scattering ( sca ), and extinction ( ext ) cross sections. These parameters have been calculated in the case of AuNPs dispersed in homogeneous media, but not for specific tissues. The aim of this research was to theoretically obtain the optical cross sections ( abs , sca , and ext ) of functionalized AuNPs in liver and colon tissues through Mie theory and correlate them with the temperature increase observed experimentally in tissues containing AuNPs under plasmonic photothermal irradiation using a Nd-YAG laser ( = 532 nm). Calculations showed that abs represents 98.96 ± 0.03% of ext at 532 nm. The ext value for a functionalized AuNP in water was 365.66 nm 2 (94% of the theoretical maximum value at 522.5 nm), 404.24 nm 2 in colon (98% of the theoretical maximum value at 525 nm), and 442.39 nm 2 in liver (96% of the theoretical maximum value at 525 nm). Therefore, nanoparticles irradiated at 532 nm are very close to their resonance value. These results correlated with the experimental irradiation of functionalized AuNPs in different tissues, where the average temperature increase showed the pattern liver > colon > water. The temperature increase observed (Δ up to 13 ∘ C) is sufficient to produce cellular death.

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“…The effect of laser heating, a well-characterized AuNP-OCT system on HeLa cell viability, was evaluated as a suitable agent for plasmonic photothermal therapy in the treatment of cervical cancer. 81 The peptide was conjugated to AuNPs (∼20 nm) by spontaneous reaction of thiol groups. HeLa cells were incubated at 37°C with AuNP-citrate, with AuNP-OCT, or without nanoparticles.…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Receptor binding peptides for target-selective delivery of nanoparticles encapsulated drugs

Accardo¹,

Aloj²,

Aurilio³

et al. 2014

IJN

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Active targeting by means of drug encapsulated nanoparticles decorated with targeting bioactive moieties represents the next frontier in drug delivery; it reduces drug side effects and increases the therapeutic index. Peptides, based on their chemical and biological properties, could have a prevalent role to direct drug encapsulated nanoparticles, such as liposomes, micelles, or hard nanoparticles, toward the tumor tissues. A considerable number of molecular targets for peptides are either exclusively expressed or overexpressed on both cancer vasculature and cancer cells. They can be classified into three wide categories: integrins; growth factor receptors (GFRs); and G-protein coupled receptors (GPCRs). Therapeutic agents based on nanovectors decorated with peptides targeting membrane receptors belonging to the GPCR family overexpressed by cancer cells are reviewed in this article. The most studied targeting membrane receptors are considered: somatostatin receptors; cholecystokinin receptors; receptors associated with the Bombesin like peptides family; luteinizing hormone-releasing hormone receptors; and neurotensin receptors. Nanovectors of different sizes and shapes (micelles, liposomes, or hard nanoparticles) loaded with doxorubicin or other cytotoxic drugs and externally functionalized with natural or synthetic peptides are able to target the overexpressed receptors and are described based on their formulation and in vitro and in vivo behaviors

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Laser Heating of Gold Nanospheres Functionalized with Octreotide: In Vitro Effect on HeLa Cell Viability

Abstract: The AuNP-octreotide system exhibited properties suitable for plasmonic photothermal therapy in the treatment of cervical cancer.

Cited by 25 publications

References 25 publications

Prostate-specific membrane antigen–directed nanoparticle targeting for extreme nearfield ablation of prostate cancer cells

Prostate-specific membrane antigen–directed nanoparticle targeting for extreme nearfield ablation of prostate cancer cells

Study of the Optical Properties of Functionalized Gold Nanoparticles in Different Tissues and Their Correlation with the Temperature Increase

Receptor binding peptides for target-selective delivery of nanoparticles encapsulated drugs

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