Fluorescence RadicalsLaser-induced fluorescence (LIF) techniques for the quantitative, point-wise measurement of radical concentrations and temperature were developed and applied in laboratory-scale combustion systems. Currcntly, the potential of similar measurement strategies for accurate two-dimensional, single-pulse experiments is being investigated. The sensitivity of typical LIF approaches for the determination of temperature and concentration to several parameters, especially to collision processes, is examined. Instantaneous two-dimensional two-species fluorescence imaging is demonstrated in a turbulent H2 diffusion flame. Implications for the quantitative interpretation of these results are discussed in view of single-pulse multi-species spontaneous Raman measurements under similar conditions.