Laser-Induced Cell Detachment, Patterning, and Regrowth on Gold Nanoparticle Functionalized Surfaces

Abstract

We report on the selective cell detachment from nanoengineered gold nanoparticle (AuNP) surfaces triggered by laser irradiation, which occurs in a nonthermal manner. The gold nanoparticle-based surfaces reveal good adhesion of NIH3T3 fibroblast cells. Patterning is achieved by lithographic microcontact printing, selective gold nanoparticle deposition, and by laser beam profiling. It is shown that the effectiveness of fibroblast cell detachment depends on the cell age, laser power, and AuNP patterning profile. Heat distribution and temperature rise around gold nanoparticle functionalized surfaces is modeled, revealing low heating of nanoparticles by laser illumination. The nonthermal photochemical mechanism of cell detachment due to production of reactive oxygen species under illumination of gold nanoparticles by green laser light is studied. We also demonstrate that cells migrate from unirradiated areas leading to their reattachment and surface recovery which is important for controlled spatial organization of cells in wound healing and tissue engineering. Research presented in this work is targeted at designing biointerfaces for cell cultures.

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

“…ROS damages cell membranes and that led to cell detachments. Furthermore, surface recovery due to migration of cells from non‐irradiated areas was observed …”

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

“…Although the need for less-invasive cell harvesting methods has been noted in the literature, only a few works, which either require electricity-induction (Yeo et al, 2001, Inaba et al, 2009, Wildt et al, 2010, Zhu et al, 2008), pH change-induction (Chen et al, 2012, Guillaume-Gentil et al, 2011), or light-induction methods (Hong et al, 2013, Pasparakis et al, 2011, Sada et al, 2011, Shin et al, 2011, Kolesnikova et al, 2012, Higuchi et al, 2004) have successfully addressed this issue. Only a few non-invasive cell detachment methods such as those that use aptamers (Wan et al, 2012, Zhang et al, 2012b, Zhu et al, 2012) or hydrogels (Plouffe et al, 2009a, Hatch et al, 2011, Hatch et al, 2012) have been shown to be successful for cell detachment.…”

Responsive systems for cell sheet detachment

“…43 Fibroblasts were cultured on gold nanoparticle based surfaces. Gold nanoparticles have strong photoabsorpotion in the green spectral range.…”