Laser-Induced Fluorescence Detection of a Single Molecule in a Capillary

Lee, Yuan-Hsiang; Maus, Russell G.; Smith, B. W.; Winefordner, J. D.

doi:10.1021/ac00095a005

Cited by 123 publications

(86 citation statements)

References 19 publications

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“…It has been maintained mainly by mechanical syringe pumps 16,17 , but the electrophoresis was used in the present work. The basic concept by Soper et al 16 and Lee et al 17 was applied to a single molecule detection of Rhodamine B 914 ANALYTICAL SCIENCES OCTOBER 1998, VOL. 14 in the flowing stream except that the present study was based on two-photon excitation.…”

Section: Single Molecule Detection Of Rhodamine Bmentioning

confidence: 99%

Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell

et al. 1998

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The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.

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Section: Single Molecule Detection Of Rhodamine Bmentioning

confidence: 99%

Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell

et al. 1998

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“…Consequently, LIF using OPO lasers is in minority, though some achievements have been reported [21][22][23]. Another alternative may be a use of tunable excimer lasers, but this is limited to species that absorb in their relatively narrow tuning range [24].…”

Section: Instrumentationmentioning

confidence: 99%

Laser-Induced Fluorescence of Hydroxyl (OH) Radical in Cold Atmospheric Discharges

Voráč¹,

Dvořák²,

Mrkvičková³

2018

Photon Counting - Fundamentals and Applications

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The application of laser-induced fluorescence (LIF) to measurement of absolute concentration of hydroxyl radicals in cold atmospheric discharges is described. Though only the case of OH is presented, the method can be directly applied to other molecules as well. Starting from the rate equations for the LIF process, the main formulas for two-and multilevel excitation scheme are derived. It is also shown how to use partially saturated LIF in practice, enhancing the signal-to-noise ratio. Practical tips for automating the data evaluation are given, allowing processing large data sets, particularly suitable for planar measurements. Gas temperature estimation from fluorescence on different rotational absorption lines is shown as an attractive method for obtaining temperature maps with high spatial resolution. The important aspects of calibration are discussed, particularly the overlap of the laser line with the selected absorption line and the measurement of the Rayleigh scattering for sensitivity calibration, together with the common sources of errors. The application of OH(A, v 0 =0 X, v 00 = 0) excitation scheme to the effluent of atmospheric pressure plasma jet ignited in argon and of OH(A, v 0 =1 X, v 00 = 0) to the plasma of coplanar surface barrier discharge in air and in water vapor is shown.

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“…The LODs of the best CE/laser-based systems are well below 10 ±13 M [11,31] producing mass detection limits of less than 10 molecules [32]. In fact, single molecule detection has been demonstrated in a capillary using LIF [33] and subsequently at the stochatic noise limit in CE [34]. Various arrangements or approaches to LIF have been implemented, including the first on-column LIF detection system developed by Zare et al [35], the postcolumn sheath flow cuvette first demonstrated by Dovichi and co-workers [36±39], and indirect LIEF reported by Yeung and Kuhr [40,41].…”

Section: Laser-induced Fluorescencementioning

confidence: 99%

“…Note that Winefordner and co-workers [33] were the first group to demonstrate single molecule detection in a capillary; however, Chen and Dovichi [34] first showed that single molecule detection could be achieved under CE conditions. More recently, the ability to discern the individual behavior and activity of enzymes has been demonstrated by the detection of reaction products generated from enzyme action by several groups [49±51] (Fig.…”

Section: Post-column Detectionmentioning

confidence: 99%