Laser induced fluorescence photobleaching anemometer for microfluidic devices

Wang, Guiren

doi:10.1039/b416209a

Cited by 47 publications

(43 citation statements)

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“…Haidekker et al [21] explained the change in emission intensity via shear stress using a fluorescent molecular rotor. Wang et al [22] argued that the change in fluorescence emission intensity was due to so-called photobleaching. We investigated the effect of photobleaching in our device configuration.…”

Section: Resultsmentioning

confidence: 99%

A Spectroscopic Technique for Local Temperature Measurement in a Micro-Optofluidic System

et al. 2016

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• A submitted manuscript is the version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publicationGeneral rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. Abstract-We present a spectroscopy technique to measure temperature locally in a polydimethylsiloxane (PDMS) microoptofluidic chip with integrated optical fibers and minimal optical components. The device was fabricated in one step with fiber coupler grooves followed by manual integration of the optical fibers. The experimental setup consists of a microoptofluidic chip with a pair of optical fibers for excitation and fluorescence collection, a laser module and a spectrometer. The laser module is coupled to one of the optical fibers to guide the light into the microchannel. The fluorescence signal is collected by a second integrated optical fiber placed orthogonally. A spectroscopy technique is used to measure the local temperature in a microchannel (500 µm wide and 125 µm in height) using Rhodamine B as a temperature indicator. It is shown that for a flow rate between 200 and 400 μL/min, the local temperature can be determined.

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Section: Resultsmentioning

confidence: 99%

A Spectroscopic Technique for Local Temperature Measurement in a Micro-Optofluidic System

et al. 2016

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“…To measure flow velocity using LIFPA for a given pressure in the microchannel, a calibration relationship between U and RFU is required [27]. This could be obtained through measuring the flush time, t f , of the dye sample with different DP provided by the vacuum pump.…”

Section: Test Of the Vacuum Pumpmentioning

confidence: 99%

“…This paper introduces the Laser Induced Fluorescence Photobleaching Anemometer (LIFPA) [27] developed recently to test the hydrodynamic pump for hydrodynamic velocity, high-voltage power supplier for EOF velocity and the biochip quality, respectively. The method provides us with an easy, fast, instantaneous, accurate, and potentially in-line velocity (or flow rate) measurement.…”

Section: Introductionmentioning

confidence: 99%

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

Wang¹,

Sas²,

Jiang³

et al. 2008

Electrophoresis

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For microfluidic analytical instruments, a facile, fast, and accurate instrument test is highly demanded. The test includes the quantitative verification of the relationship between pressure drop and flow velocity for the hydrodynamic pump, between the electric voltage and electroosmotic flow (EOF) for the high-voltage supply, and the chip quality. The key point for the test is the measurement of the flow velocity. However, most currently available velocimetries cannot be directly used without any instrumental modification or adding extra instruments. We applied a recently developed Laser Induced Fluorescence Photobleaching Anemometer (LIFPA) for the instrument test through measuring fluid flow velocity in a microfluidic instrument with optical measurement without any modification and extra instrument. We have successfully used the method to test Caliper HTS 250 System from Caliper Life Sciences (Hopkinton, MA) with its own light source and detector. The experimental result demonstrates that this single-point method of measuring flow velocity can be easily used for accurate test of a microfluidic instrument in less than 10 min at extremely low cost without any modification and extra instrument.

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“…[40][41][42][43] In LIFPA, a molecular tracer of fluorescence dye and the photobleaching effect are applied as a transducer to measure the flow velocity. The velocity is calculated by measuring fluorescence with a calibration relationship between the velocity and fluorescence.…”

Section: Introductionmentioning

confidence: 99%

A novel far-field nanoscopic velocimetry for nanofluidics

Kuang

Wang

2010

Lab Chip

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For the first time we have been able to measure the flow velocity profile for nanofluidics with a spatial resolution better than 70 nm. Due to the diffraction resolution barrier, traditional optical methods have so far failed in measuring the velocity profile in a nanocapillary or a closed nanochannel without an opened sidewall. A novel optical point measurement method is presented which applies stimulated emission depletion (STED) microscopy to laser induced fluorescence photobleaching anemometer (LIFPA) techniques to measure flow velocity. Herein we demonstrate this far-field nanoscopic velocimetry method by measuring the velocity profile in a nanocapillary with an inner diameter of 360 nm. The closest measuring point to the wall is about 35 nm. This method opens up a new class of functional measuring techniques for nanofluidics and for nanoscale flows from the wall.

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Laser induced fluorescence photobleaching anemometer for microfluidic devices

Cited by 47 publications

References 50 publications

A Spectroscopic Technique for Local Temperature Measurement in a Micro-Optofluidic System

A Spectroscopic Technique for Local Temperature Measurement in a Micro-Optofluidic System

Photobleaching‐based flow measurement in a commercial capillary electrophoresis chip instrument

A novel far-field nanoscopic velocimetry for nanofluidics

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