Laser induced fluorescence spectroscopy of various carbon nanostructures (GO, G and nanodiamond) in Rd6G solution

Abstract

Abstract:The effect of carbon nanostructures such as graphene (G), graphene oxide (GO) and nanodiamond (ND) on the spectral properties of Rhodamine 6G (Rd6G) emission due to the laser induced fluorescence (LIF) was investigated. It is shown that the addition of carbon nanostructures lead to sensible Red/Blue shifts which depend on the optical properties and surface functionality of nanoparticles. The current theories such as resonance energy transfer (RET), fluorescence quenching and photon propagation in scattering media support the experimental findings. Stern-Volmer curves for dynamic and static quenching of Rd6G molecules embedded with G, GO and nanodiamond are correlated with spectral shifts. Furthermore, time evolution of the spectral shift contributes to determine loading/release rates of fluorescent species with large S-parameter on the given nano-carriers.

Fluorescence properties of several chemotherapy drugs: doxorubicin, paclitaxel and bleomycin

“…In this way, the interplay between the Stokes shift and overlapping area are exhibited in a definitive way. Figures 2(a), 2(c) and 2(e) illustrate the emissive wavelengths in terms of concentrations where the red shift usually realizes as a function of fluorescence concentration [17]. Similarly, the emissive wavelengths follow an initial rise due to the reabsorption events and then exhibit a plateau at higher fluorophore concentrations.…”

LIF spectroscopy of stained malignant breast tissues

“…The nature of the short penetration depth at 405nm, as well as the relatively small overlapping region between the C7 absorption and the fluorescence emissions, intensify this result. On the other hand, the absorption coefficient of the Rd6G is lucidly large at 532 nm [13,16], thus the photons undergo more reabsorption events, leading to a sensible red shift, in comparison.…”

“…The formation of the numerous micro-cavities is initiated at the onset of the abnormal cell proliferation due to the structural alteration of tubular architecture [38]. The latter resembles to induce the micro-partitioning effect [16,32]. Here, the micropartitioning architecture of the cancerous scaffold slows down the aggregation events such that the micro-cavities do trap further fluorophore molecules.…”

“…This leads mainly to a further self-quenching. On the other hand, the small Stocks shift usually induces a notable spectral shift [13,16], which is the focus of our work, where distinct characteristics of several fluorophores such as Rd6G are sought.…”

A spectrometer based on smartphones and a low-cost kit for transmittance and absorbance measurements in real-time

“…We prepared ethanol solutions of Rhodamine 6G, which is an inexpensive a highly fluorescent dye with a well-known absorption spectrum [11].…”