Laser induced self-N-doped porous graphene as an electrochemical biosensor for femtomolar miRNA detection

Zhang, Wan; Umer, Muhammad; Lobino, Mirko; Thiel, David V.; Nguyen, Nam‐Trung; Trinchi, Adrian; Shiddiky, Muhammad J. A.; Gao, Yongsheng; Q, Li

doi:10.1016/j.carbon.2020.03.043

Cited by 147 publications

(102 citation statements)

References 75 publications

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“…Benefiting from large surface areas and rich surface defects induced during the laser scribing process, pristine LIG-based devices have been widely explored for detections of small molecules [50,[86][87][88][89][90] and nucleic acids [91] in biofluids (e.g., sweat, urine, saliva). For instance, Xu et al have developed the electrochemical neurotransmitter sensors based on grass-like LIG, which can selectively detect dopamine (DA), epinephrine (EP) and norepinephrine (NE) using the differential pulse voltammetry (DPV) method [87].…”

Section: Biofluids Biochemical Sensorsmentioning

confidence: 99%

“…In this study, in vivo demonstrations are also provided for gout management, indicating promising applications of LIG-based biochemical sensors for personalized sweat analysis. In addition, the ability of LIG-based biochemical sensors to detect other analytes in biofluids, such as sodium sulfate, hydrazine, miRNA, and lead (Pb), have also been recently reported [88][89][90][91].…”

Section: Biofluids Biochemical Sensorsmentioning

confidence: 99%

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Laser-induced graphene for bioelectronics and soft actuators

Fei

Page

et al. 2021

Nano Res.

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Laser-assisted process can enable facile, mask-free, large-area, inexpensive, customizable, and miniaturized patterning of laser-induced porous graphene (LIG) on versatile carbonaceous substrates (e.g., polymers, wood, food, textiles) in a programmed manner at ambient conditions. Together with high tailorability of its porosity, morphology, composition, and electrical conductivity, LIG can find wide applications in emerging bioelectronics (e.g., biophysical and biochemical sensing) and soft robots (e.g., soft actuators). In this review paper, we first introduce the methods to make LIG on various carbonaceous substrates and then discuss its electrical, mechanical, and antibacterial properties and biocompatibility that are critical for applications in bioelectronics and soft robots. Next, we overview the recent studies of LIG-based biophysical (e.g., strain, pressure, temperature, hydration, humidity, electrophysiological) sensors and biochemical (e.g., gases, electrolytes, metabolites, pathogens, nucleic acids, immunology) sensors. The applications of LIG in flexible energy generators and photodetectors are also introduced. In addition, LIG-enabled soft actuators that can respond to chemicals, electricity, and light stimulus are overviewed. Finally, we briefly discuss the future challenges and opportunities of LIG fabrications and applications.

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Section: Biofluids Biochemical Sensorsmentioning

confidence: 99%

Section: Biofluids Biochemical Sensorsmentioning

confidence: 99%

Laser-induced graphene for bioelectronics and soft actuators

Fei

Page

et al. 2021

Nano Res.

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“…In another investigation, femtomolar miRNA detection was reported using laser induced self-N-doped porous graphene as an electrochemical biosensor. 89 In order to detect the target miRNAs, they were puried with laser induced graphene sensor using miRNA extraction and magnetic isolation process. The results obtained revealed the detection of miRNA until the concentration reaches 10 fM.…”

Section: Nitrogen-doped Graphene-based Sensorsmentioning

confidence: 99%

Heteroatom-doped graphene as sensing materials: a mini review

et al. 2020

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“…Wan’s group used a screen-printed graphene electrode, for miRNAs detection isolated and directly adsorbed on the surface of the graphene electrode via graphene–miRNA affinity interaction. This method showed an LOD of 10 fM [ 177 ]. According to the present two works, we can conclude that graphene–miRNA affinity is higher than the gold–miRNA affinity.…”

Section: Electrochemical Label-free Biosensingmentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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Laser induced self-N-doped porous graphene as an electrochemical biosensor for femtomolar miRNA detection

Cited by 147 publications

References 75 publications

Laser-induced graphene for bioelectronics and soft actuators

Laser-induced graphene for bioelectronics and soft actuators

Heteroatom-doped graphene as sensing materials: a mini review

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

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