Laser radiation effect on chondrocytes and intercellular matrix of costal and articular cartilage impregnated with magnetite nanoparticles

Soshnikova, Yulia M.; Shekhter, Anatoly B.; Baum, Olga I.; Shcherbakov, Evgeniy M.; Omelchenko, Alexander I.; Лунин, В. В.; Sobol, Emil N.

doi:10.1002/lsm.22331

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…If cartilage tissue has small defects or undergoes laser irradiation with specific pulse‐periodic regime the distribution of pores can be locally significantly extended and the tissue becomes available for the impregnation of larger particles up to 300 nm . The safety of the used NPs for cartilage cells and matrix was shown in . The morphology and crystalline structure of the obtained NPs as well as their thermal and storage stability were analyzed in .…”

Section: Resultsmentioning

confidence: 99%

“…To increase the absorption of the superficial layers of cartilage, we proposed to use clinically approved absorbing magnetite nanoparticles (NPs) modified with biopolymer that enhances their biocompatibility and stability . Impregnation of NPs in avascular cartilage is possible due to the existence of submicron pores in its structure . It was shown that the time of NPs impregnation and extraction can be accelerated by application of magnetic field .…”

Section: Introductionmentioning

confidence: 99%

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Controlling the near‐infrared transparency of costal cartilage by impregnation with clearing agents and magnetite nanoparticles

Alexandrovskaya

Sadovnikov

Sharov

et al. 2017

Journal of Biophotonics

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Penetration depth of near-infrared laser radiation to costal cartilage is controlled by the tissue absorption and scattering, and it is the critical parameter to provide the relaxation of mechanical stress throughout the whole thickness of cartilage implant. To enhance the penetration for the laser radiation on 1.56 μm, the optical clearing solutions of glycerol and fructose of various concentrations are tested. The effective and reversible tissue clearance was achieved. However, the increasing absorption of radiation should be concerned: 5°C-8°C increase of tissue temperature was detected. Laser parameters used for stress relaxation in cartilage should be optimized when applying optical clearing agents. To concentrate the absorption in the superficial tissue layers, magnetite nanoparticle (NP) dispersions with the mean size 95 ± 5 nm and concentration 3.9 ± 1.1 × 10 particles/mL are applied. The significant increase in the tissue heating rate was observed along with the decrease in its transparency. Using NPs the respective laser power can be decreased, allowing us to obtain the working temperature locally with reduced thermal effect on the surrounding tissue.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controlling the near‐infrared transparency of costal cartilage by impregnation with clearing agents and magnetite nanoparticles

Alexandrovskaya

Sadovnikov

Sharov

et al. 2017

Journal of Biophotonics

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“…The proteoglycan structure has an electrical nature and positive structure provided by positive ions dissolved in tissue water [ 19 ]. The electrical repulsion of the negatively charged groups is an origin of the stress bringing cartilage sample to its initial shape [ 20 , 21 ]. Therefore, calcified cartilage had poor deformation under the same stress, compared with non-calcified costal cartilage.…”

Section: Discussionmentioning

confidence: 99%

Mechanical properties of extensive calcified costal cartilage: An experimental study

Wang¹,

Dong²,

Wang³

et al. 2023

Heliyon

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“…The principle working parameter for effective stress relaxation is sufficient temperature. The laser power used in present work was optimized as a result of our previous investigations on ex vivo and in vivo tissue for short‐term generation of the needed temperature field avoiding substantial cell death and collagen denaturation [3,5–7,9,28]. As it is shown here the average laser‐induced temperature for aged human cartilage is even lower than for the healthy one and needs increasing factor to match the optimized temperature.…”

Section: Discussionmentioning

confidence: 99%

Infrared Laser Effect on Healthy and Ossified Costal Cartilage: The Development of Stable Load‐Bearing Autoimplants

et al. 2020

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Background and Objective The laser‐induced stress relaxation provides new prospects to obtain stable long fragments of costal cartilage for autoimplantation avoiding the risk of spontaneous deformation and poor engraftment. However, the age‐related alterations of cartilage may sufficiently influence its interaction with infrared (IR) laser radiation and disrupt the effectiveness and safety of the technique. The aim of the work is to study the influence of the structural quality of costal cartilage on its interaction with IR laser and efficiency of obtaining of curved implants for trachea surgery. Study Design/Materials and Methods Healthy costal cartilage was taken from pigs and human. Ossified costal cartilage was taken from humans of age 65 ± 7. The cartilage slices with a mean thickness of 3 mm were mechanically curved and processed to stress relaxation by laser irradiation with the wavelength 1.56 µm. The structure and mineral content were studied by X‐ray microtomography and element analysis. The optical measurements included the study of the propagation of IR radiation, speckle interferometry, and IR radiometry. Results The aged cartilage demonstrates a high level of heterogeneity in structure and properties and decreased water content. The presence of dense inclusions consisting of amorphous calcined volumes makes the tissue more fragile and less elastic. The IR radiation propagation intensity for aged cartilage is at least twice higher than that for healthy cartilage. The thermal‐induced motion of scatterers in aged cartilage is slower. X‐ray microtomography showed the cartilage‐like and the bone‐like structures within the ossified samples. Conclusions The main challenge for laser reshaping of aged cartilage is the presence of ossifications. However, the new stable curvature can be obtained with adjustment of laser power. To obtain the satisfying stable curvature of an implant the ossified volumes should be avoided The laser‐induced stress‐relaxation mechanism for aged cartilage can be particularly different from that of healthy tissue and the optimal laser regimes should be specified. Lasers Surg. Med. © 2020 Wiley Periodicals, Inc.

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Laser radiation effect on chondrocytes and intercellular matrix of costal and articular cartilage impregnated with magnetite nanoparticles

Cited by 6 publications

References 25 publications

Controlling the near‐infrared transparency of costal cartilage by impregnation with clearing agents and magnetite nanoparticles

Controlling the near‐infrared transparency of costal cartilage by impregnation with clearing agents and magnetite nanoparticles

Mechanical properties of extensive calcified costal cartilage: An experimental study

Infrared Laser Effect on Healthy and Ossified Costal Cartilage: The Development of Stable Load‐Bearing Autoimplants

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