Laser scanning cytometry distinguishes lymphocytes, monocytes, and granulocytes by differences in their chromatin structure

Clinical applications of laser scanning cytometry

“…Therefore, staining with 7-AAD partially replaces staining by an antibody. Similar results have been shown for PI (66). Triggering on the nucleus promises to yield reliable data on cells even in pathological states where cellular morphology and shape might be altered due to disease.…”

ASB-2 Inhibits Growth and Promotes Commitment in Myeloid Leukemia Cells

“…Fluorescence Measurement-Cellular red and green fluorescence intensities were measured by laser scanning cytometer (CompuCyte) as described (29,30). DNA content frequency histograms were deconvoluted to obtain the percentage of cells in different phases of the cell cycle.…”

“…The percentage of cells with condensed chromatin, i.e. with increased intensity of the DNA-associated maximal pixel PI fluorescence and decreased nuclear area, was obtained by gating analysis of the bivariate red integrated fluorescence versus maximal red fluorescence pixel distributions (29). The frequency of apoptotic cells was estimated from the bivariate distributions of PI versus fluorescein isothiocyanate integrated fluorescence intensities (28).…”

Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes

“…6). It is quite evident that lacking G 0 cells, the HL-60 cell population, compared to lymphocytes ( 24,48, and 72 h were subjected to analysis of nucleoli and nucleolin expression by LSC, using two different approaches. First, the integration contour was set based on red fluorescence of PI (nuclear outline), which allowed us to integrate the green immunofluorescence of nucleolin from over the whole nucleus.…”