Laser videofluorometer system for real-time characterization of high-performance liquid chromatographic eluate

Skoropinski, D. B.; Callis, James B.; Danielson, Jennifer; Christian, Gary D.

doi:10.1021/ac00126a054

Cited by 8 publications

(2 citation statements)

References 26 publications

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“…Mathmatically, the trilinear model is expressed as in eqn. ( 2), (2) where x ijk is the ijk th element of the I 3 J 3 K three-way data array X, â ir , bIRjr and ĉkr are the i th , j th and k th row of the r th column of the estimated factor loading matrices Â, B and Ĉ, R is the rank the model and e ijk is the error in the model. The PARAFAC algorithm decomposes trilinear data in to R sets of triads, which make up the matrices Â, B and Ĉ with the dimensions I 3 R, J 3 R and K 3 R, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescence excitation emission matrix (EEM) spectroscopy has long been known as a powerful method for complex mixture analysis. [1][2][3] The ability to easily collect full emission spectra for several excitation wavelengths, has generally required expensive and complex instrumentation. The multi-way characteristics of EEM data enable the extraction of the salient chemical features.…”

Section: Introductionmentioning

confidence: 99%

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Light emitting diode excitation emission matrix fluorescence spectroscopy

Hart

JiJi

2002

Analyst

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An excitation emission matrix (EEM) fluorescence instrument has been developed using a linear array of light emitting diodes (LED). The wavelengths covered extend from the upper UV through the visible spectrum: 370-640 nm. Using an LED array to excite fluorescence emission at multiple excitation wavelengths is a low-cost alternative to an expensive high power lamp and imaging spectrograph. The LED-EEM system is a departure from other EEM spectroscopy systems in that LEDs often have broad excitation ranges which may overlap with neighboring channels. The LED array can be considered a hybrid between a spectroscopic and sensor system, as the broad LED excitation range produces a partially selective optical measurement. The instrument has been tested and characterized using fluorescent dyes: limits of detection (LOD) for 9,10-bis(phenylethynyl)-anthracene and rhodamine B were in the mid parts-per-trillion range; detection limits for the other compounds were in the low parts-per-billion range (< 5 ppb). The LED-EEMs were analyzed using parallel factor analysis (PARAFAC), which allowed the mathematical resolution of the individual contributions of the mono- and dianion fluorescein tautomers a priori. Correct identification and quantitation of six fluorescent dyes in two to six component mixtures (concentrations between 12.5 and 500 ppb) has been achieved with root mean squared errors of prediction (RMSEP) of less than 4.0 ppb for all components.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%