Pollen analysis has been carried out on a 549 cm thick sediment profile from lake Plešné jezero (Plešné Lake) in the Bohemian Forest (Šumava, Czech Republic; 1090 m a.s.l.; 48• 47 N; 13• 52 E). Analyses of 67 samples characterise the development of the lake biotope and the surrounding landscape during the last ca. 14,000 years. The pollen diagram shows a very distinct transition between the Late Glacial and the Holocene biostratigraphic units at a depth of ca. 312 cm. In the surroundings of Plešné Lake the vegetation was treeless during the entire Late Glacial. The alpine tree limit, formed by Betula and Pinus with undergrowth of shrubs, might have been at ca. 500 m a.s.l. Pollen transported from long distances was significant due to the openness of the landscape, coming from southern Europe and even Africa, and including high numbers of Artemisia, Poaceae, Chenopodiaceae, and some other herbs and shrubs from steppe and forest-steppe areas in southern Europe or Africa (likely Ephedra, certainly Lygeum spartum). The expansion of shrubs, particularly Juniperus, preceded the expansion of trees near the end of the Late Glacial. Afforestation of the region by thin stands of Betula and Pinus occurred during the Preboreal. Significant warming in the Boreal resulted in the expansion of Corylus, Quercetum mixtum (QM) trees, and probably also Picea and Alnus. Picea as well as QM trees were further expanding during the Early Atlantic. Picea was the dominant tree during the Late Atlantic and Fagus started to spread towards its end. Abrupt expansion of Abies marks the Subboreal. A high degree of afforestation (Abies, Fagus, Picea) was characteristic for the Early Subatlantic. During Late Subatlantic, pollen of synanthropic plants appears. Phases of the lake biotope development were defined on the basis of coccal green algae and Isoëtes.