The whole territory of Bohemia and Moravia with only two exceptions was located in the periglacial region during the Quaternary glacial periods. These exceptions were the northernmost part of Bohemia and the mouth of the Moravian Gate through which the continental glacier in the past penetrated this country and left traces in the form of moraines and in Moravia also sediments in the oldest Czech lake, Stonava. On the basis of pollen analysis, the organic sediments recovered there were dated to the Holsteinian Interglacial (Mindel/Riss). Natural lakes are rare in the Czech Republic (Černé, Čertovo, Prášilské, Plešné, Laka, Stonava, Komořany, Chrást, Stará Boleslav, Polabské černavy, Soos, Měňany, Švarcenberk, and Velanská cesta), since most of its territory has never been glaciated. As indicated by new palynological research, this statement does not fully apply to southern Moravia, where a number of lakes existed in the past, e.g. the former lakes Vracov, Čejčské, Kobylské, Vacenovice and Vlkoš. The deposition of lake sediments occurred mainly in the Holocene. According to the present research on the Čejčské Lake, the vegetation assemblages consist of species with various ecological requirements. Due to favorable circumstances, this site has been put forward for protection and designated a Natural Monument.•