Late Effects of Whole or Partial Body X-irradiation on Mice: Life Shortening

Sato, Fumiaki; Sasaki, Shunsaku; Kawashima, Noritaka; Chino, Fumitoshi

doi:10.1080/09553008114550731

Cited by 25 publications

(21 citation statements)

References 10 publications

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“…LD50/ 30 is the whole-body radiation dose that would kill 50% of an exposed population within 30 days of exposure. For a mouse, the lethal dose ranges from 5.0 to 7.6 Gy, depending on the strain of mouse and age at exposure (16)(17)(18). The effects of sublethal radiation doses become more significant for studies involving a series of images of the same animals over time.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of the radiation dose in micro‐CT with optimization of the scan protocol

Willekens¹,

Buls²,

Lahoutte³

et al. 2010

Contrast Media & Molecular

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Section: Discussionmentioning

confidence: 98%

Evaluation of the radiation dose in micro‐CT with optimization of the scan protocol

Willekens¹,

Buls²,

Lahoutte³

et al. 2010

Contrast Media & Molecular

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“…The LD 50/30 for a mouse is reported to be approximately 700 cGy, 24,25 and the daily dose given in typical fractionated radiation therapy is approximately 200 cGy. 26 Therefore, even with repeated scans for longitudinal studies or a 40 second perfusion scan (27 cGy), we are well below these values in terms of the radiation dose given to the animal.…”

Section: Discussionmentioning

confidence: 99%

Image quality assessment of a pre-clinical flat-panel volumetric micro-CT scanner

Lee

Holdsworth

2006

SPIE Proceedings

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Small animal imaging has recently become an area of increased interest because more human diseases can be modeled in transgenic and knockout rodents. Current micro-CT systems are capable of achieving spatial resolution on the order of 10 µm, giving highly detailed anatomical information. However, the speed of data acquisition of these systems is relatively slow, when compared with clinical CT systems. Dynamic CT perfusion imaging has proven to be a powerful tool clinically in detecting and diagnosing cancer, stroke, pulmonary and ischemic heart diseases. In order to perform this technique in mice and rats, quantitative CT images must be acquired at a rate of at least 1 Hz. Recently, a research pre-clinical CT scanner (eXplore Ultra, GE Healthcare) has been designed specifically for dynamic perfusion imaging in small animals. Using an amorphous silicon flat-panel detector and a clinical slip-ring gantry, this system is capable of acquiring volumetric image data at a rate of 1 Hz, with in-plane resolution of 150 µm, while covering the entire thoracic region of a mouse or whole organs of a rat. The purpose of this study was to evaluate the principal imaging performance of the micro-CT system, in terms of spatial resolution, image uniformity, linearity, dose and voxel noise for the feasibility of imaging mice and rats. Our investigations show that 3D images can be obtained with a limiting spatial resolution of 2.7 line pairs per mm and noise of 42 HU, using an acquisition interval of 8 seconds at an entrance dose of 6.4 cGy.

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“…This delicate balance of x-ray energy, absorbed dose, and image noise leads to limitations in both the contrast and resolution for micro-CT of small animals. Ford et al [8] have argued that, for an entrance dose of 5.0 Gy (very nearly the lethal dose for whole body irradiation [9]), a minimum voxel size of about 65 microns was possible when imaging mice.…”

Section: Conventional Micro-ct Of Small Animalsmentioning

confidence: 99%

Energy and dose considerations for diffraction enhanced CT in small animal studies

et al. 2007

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Diffraction enhanced imaging (DEI) uses monochromatic x-rays coupled to an analyzer crystal to extract information about the refraction of x-rays within the object. Studies of excised biological tissues show that DEI has significant contrast-to-noise ratio (CNR) advantages for soft tissue when compared to standard radiography. DEI differs from conventional CT in that its refraction contrast depends on x-ray energy as 1/E, thus the energy and dose considerations for conventional CT will be inappropriate. The goal of this study was to assess the optimal energy for in vivo CT imaging of a mouse head to obtain the largest soft tissue refraction CNR. Through a theoretical model, optimum refraction CNR for mouse brain imaging was found to be about 20 keV. The findings were tested experimentally using the DEI system at the X15A beamline of the National Synchrotron Light Source. Using the parameters for optimized refraction CNR (20 keV, silicon [333] reflection), large image artifacts were caused by DEI's scatter-rejection properties. By increasing the x-ray energy and using a lower order diffraction, silicon [111], soft tissue features within the brain, including the hippocampus, could be resolved.

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Late Effects of Whole or Partial Body X-irradiation on Mice: Life Shortening

Cited by 25 publications

References 10 publications

Evaluation of the radiation dose in micro‐CT with optimization of the scan protocol

Evaluation of the radiation dose in micro‐CT with optimization of the scan protocol

Image quality assessment of a pre-clinical flat-panel volumetric micro-CT scanner

Energy and dose considerations for diffraction enhanced CT in small animal studies

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