Lateral Degassing Method for Disposable Film-Chip Microfluidic Devices

Park, Suhee; Cho, Hyungseok; Kim, Junhyeong; Han, Ki-Ho

doi:10.3390/membranes11050316

Cited by 8 publications

(5 citation statements)

References 54 publications

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“…Once formed, bubbles are extremely difficult to remove and can cause flow instabilities and degrade the microfluidic device's performance. [ 54 ] However, this problem has been eliminated with the developed PhSC because the photocrosslinking is a fast reaction that does not cause any thermal changes during crosslinking and therefore no bubbles are formed in the development of microfluidic devices.…”

Section: Resultsmentioning

confidence: 99%

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Alioglu,

Yilmaz,

Gerhard

et al. 2023

Adv Materials Technologies

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Embedded printing has emerged as a valuable tool for fabricating complex structures and microfluidic devices. Currently, an ample of amount of research is going on to develop new materials to advance its capabilities and increase its potential applications. Here, a novel, transparent, printable, photocrosslinkable, and tuneable silicone composite is demonstrated that can be utilized as a support bath or an extrudable ink for embedded printing. Its properties can be tuned to achieve ideal rheological properties, such as optimal self‐recovery and yield stress, for use in 3D printing. When used as a support bath, it facilitated the generation microfluidic devices with circular channels of diameter up to 30 µm. To demonstrate its utility, flow focusing microfluidic devices are fabricated for generation of Janus microrods, which can be easily modified for multitude of applications. When used as an extrudable ink, 3D printing of complex‐shaped constructs are achieved with integrated electronics, which greatly extends its potential applications toward soft robotics. Further, its biocompatibility is tested with multiple cell types to validate its applicability for tissue engineering. Altogether, this material offers a myriad of potential applications (i.e., soft robotics, microfluidics, bioprinting) by providing a facile approach to develop complicated 3D structures and interconnected channels.

show abstract

Section: Resultsmentioning

confidence: 99%

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Alioglu,

Yilmaz,

Gerhard

et al. 2023

Adv Materials Technologies

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show abstract

“…Nevertheless, PDMS devices have some limitations, such as a labor-intensive fabrication process [100] and problems due to leakage and/or unbalanced pressure among chambers [101]. Air bubbles removal is a further limitation in closed PDMS devices, which can be overcome by using effective but complex fluidic designs that might include open channels and/or semipermeable membrane for degassing [77,[102][103][104][105][106]. This issue is particularly relevant for IR plasmonics, as local thermally-driven outgassing is a confounding agent in interpreting results.…”

Section: Discussionmentioning

confidence: 99%

Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures

et al. 2022

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Background Extracellular Vesicles (EVs) are sub-micrometer lipid-bound particles released by most cell types. They are considered a promising source of cancer biomarkers for liquid biopsy and personalized medicine due to their specific molecular cargo, which provides biochemical information on the state of parent cells. Despite this potential, EVs translation process in the diagnostic practice is still at its birth, and the development of novel medical devices for their detection and characterization is highly required. Results In this study, we demonstrate mid-infrared plasmonic nanoantenna arrays designed to detect, in the liquid and dry phase, the specific vibrational absorption signal of EVs simultaneously with the unspecific refractive index sensing signal. For this purpose, EVs are immobilized on the gold nanoantenna surface by immunocapture, allowing us to select specific EV sub-populations and get rid of contaminants. A wet sample-handling technique relying on hydrophobicity contrast enables effortless reflectance measurements with a Fourier-transform infrared (FTIR) spectro-microscope in the wavelength range between 10 and 3 µm. In a proof-of-principle experiment carried out on EVs released from human colorectal adenocarcinoma (CRC) cells, the protein absorption bands (amide-I and amide-II between 5.9 and 6.4 µm) increase sharply within minutes when the EV solution is introduced in the fluidic chamber, indicating sensitivity to the EV proteins. A refractive index sensing curve is simultaneously provided by our sensor in the form of the redshift of a sharp spectral edge at wavelengths around 5 µm, where no vibrational absorption of organic molecules takes place: this permits to extract of the dynamics of EV capture by antibodies from the overall molecular layer deposition dynamics, which is typically measured by commercial surface plasmon resonance sensors. Additionally, the described metasurface is exploited to compare the spectral response of EVs derived from cancer cells with increasing invasiveness and metastatic potential, suggesting that the average secondary structure content in EVs can be correlated with cell malignancy. Conclusions Thanks to the high protein sensitivity and the possibility to work with small sample volumes—two key features for ultrasensitive detection of extracellular vesicles- our lab-on-chip can positively impact the development of novel laboratory medicine methods for the molecular characterization of EVs. Graphical Abstract

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“…Although photocrosslinking occurred within a minute, PhSC was exposed to light for 10 min to ensure fully crosslinking. In the literature, one of the challenges of silicone-based microfluidic device fabrication is the need for degassing after casting 24 . However, this problem was eliminated with PhSC because reaction occurred rapidly without a thermal change and without bubbles generation, and thus the degassing step after casting was not required.…”

Section: Development Of Phsc Materials For Embedded Printing Of Micro...mentioning

confidence: 99%

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Alioglu,

Yilmaz,

Gerhard

et al. 2023

Preprint

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Embedded printing has emerged as a valuable tool for fabricating complex structures and microfluidic devices. Currently, an ample of amount of research is going on to develop new materials to advance its capabilities and increase its potential applications. Here, we demonstrate a novel, transparent, 3D printable, photocrosslinkable, and tuneable silicone composite that can be utilized as a support bath or an extrudable ink for embedded printing. The proposed silicone composite can be tuned to achieve ideal rheological properties, such as optimal self-recovery and yield stress, for use in 3D printing. When used as a support bath, it facilitated the generation microfluidic devices with circular channels of diameter up to 30 microns. To demonstrate its utility, flow focusing microfluidic devices were fabricated for generation of Janus microrods, which can be easily modified for multitude of applications. When used as an extrudable ink, 3D printing of complex-shaped micro- and macro-constructs were achieved with integrated electronics, which greatly extends its potential applications towards developing complex flexible parts for soft robotics and prosthetics. Further, its biocompatibility was tested with multiple cell types to validate its applicability for medical and tissue engineering use. Altogether, this material offers a myriad of potential applications in material and medical fields by providing a facile approach to develop complicated 3D structures and interconnected channels that can further advance microfluidics and soft-robotics research.

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Lateral Degassing Method for Disposable Film-Chip Microfluidic Devices

Cited by 8 publications

References 54 publications

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

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