Lateral diffusion in the plasma membrane of maize protoplasts with implications for cell culture

Dugas, Catherine M.; Li, Quanning; Khan, Iarar A.; Nothnagel, Eugene A.

doi:10.1007/bf02411462

Cited by 3 publications

(4 citation statements)

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“…At the end of the culture period, live cells were identified using fluorescein diacetate (FDA) as described by Dugas et al (1989). Aliquots of cell suspensions were treated with FDA and then observed through a fluorescence microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Lateral diffusion measurements of lipids, proteins, and glycoconjugates of the plasma membrane of protoplasts were made by fluorescence photobleaching recovery (Li and Nothnagel 1988;Dugas et al 1989;Walko and Nothnagel 1989). In brief, protoplasts were fluorescence-labeled on the plasma membrane and then examined under a fluorescence microscope where a laser beam was brought to a 1-~m-radius focus on the plasma membrane.…”

Section: Measurements Of Lateral Diffusion Of Plasma-membrane Componementioning

confidence: 99%

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Effects of Yariv phenylglycosides onRosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation

Serpe

Nothnagel

1994

Planta

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171

161

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Section: Methodsmentioning

confidence: 99%

Section: Measurements Of Lateral Diffusion Of Plasma-membrane Componementioning

confidence: 99%

Effects of Yariv phenylglycosides onRosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation

Serpe

Nothnagel

1994

Planta

Self Cite

171

161

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“…The induced rigidity of the plasma membrane causes the microtubules to dissociate from the plasma membrane (Ö rvar et al 2000;Sangwan et al 2001). In addition, low-temperature stress increases the diffusion of the lipids in the plasma membrane and causes depolymerization of the microtubules (Dugas et al 1989). Thus, chemical fixation at 2-3°C might depolymerize the microtubules in cambial cells and their derivatives through changes in the properties of the plasma membrane.…”

Section: Discussionmentioning

confidence: 98%

Cold stability of microtubules in wood-forming tissues of conifers during seasons of active and dormant cambium

Begum

Shibagaki

Furusawa

et al. 2011

Planta

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The cold stability of microtubules during seasons of active and dormant cambium was analyzed in the conifers Abies firma, Abies sachalinensis and Larix leptolepis by immunofluorescence microscopy. Samples were fixed at room temperature and at a low temperature of 2-3°C to examine the effects of low temperature on the stability of microtubules. Microtubules were visible in cambium, xylem cells and phloem cells after fixation at room temperature during seasons of active and dormant cambium. By contrast, fixation at low temperature depolymerized microtubules in cambial cells, differentiating tracheids, differentiating xylem ray parenchyma and phloem ray parenchyma cells during the active season. However, similar fixation did not depolymerize microtubules during cambial dormancy in winter. Our results indicate that the stability of microtubules in cambial cells and cambial derivatives at low temperature differs between seasons of active and dormant cambium. Moreover, the change in the stability of microtubules that we observed at low temperature might be closely related to seasonal changes in the cold tolerance of conifers. In addition, low-temperature fixation depolymerized microtubules in cambial cells and differentiating cells that had thin primary cell walls, while such low-temperature fixation did not depolymerize microtubules in differentiating secondary xylem ray parenchyma cells and tracheids that had thick secondary cell walls. The stability of microtubules at low temperature appears to depend on the structure of the cell wall, namely, primary or secondary. Therefore, we propose that the secondary cell wall might be responsible for the cold stability of microtubules in differentiating secondary xylem cells of conifers.

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“…In an animal system, Aszalos et al (1) found that microtubule depolymerization increased the fluidity of the plasma membrane, a change associated with increased low temperature tolerance in plants. Similarly, in maize protoplasts microtubule depolymerization increased the lateral diffusion of membrane lipids and proteins (6).…”

mentioning

confidence: 96%

Effect of Microtubule Stabilization on the Freezing Tolerance of Mesophyll Cells of Spinach

Bartolo¹,

Carter²

1991

Plant Physiol.

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Freezing, dehydration, and supercooling cause microtubules in mesophyll cells of spinach (Spinacia oleracea L. cv Bloomsdale) to depolymerize (ME Bartolo, JV Carter, Plant Physiol [1991] 97: 175-181). The objective of this study was to determine whether the LT50 (lethal temperature: the freezing temperature at which 50% of the tissue is killed) of spinach leaf tissue can be changed by diminishing the extent of microtubule depolymerization in response to freezing. Also examined was how tolerance to the components of extracellular freezing, low temperature and dehydration, is affected by microtubule stabilization. Leaf sections of nonacclimated and cold-acclimated spinach were treated with 20 micromolar taxol, a microtubule-stabilizing compound, prior to freezing, supercooling, or dehydration. Taxol stabilized microtubules against depolymerization in cells subjected to these stresses. When pretreated with taxol both nonacclimated and cold-acclimated cells exhibited increased injury during freezing and dehydration. In contrast, supercooling did not injure cells with taxol-stabilized microtubules. Electrolyte leakage, visual appearance of the cells, or a microtubule repolymerization assay were used to assess injury. As leaves were cold-acclimated beyond the normal period of 2 weeks taxol had less of an effect on cell survival during freezing. In leaves acclimated for up to 2 weeks, stabilizing microtubules with taxol resulted in death at a higher freezing temperature. At certain stages of cold acclimation, it appears that if microtubule depolymerization does not occur during a freeze-thaw cycle the plant cell will be killed at a higher temperature than if microtubule depolymerization proceeds normally. An alternative explanation of these results is that taxol may generate abnormal microtubules, and connections between microtubules and the plasma membrane, such that normal cellular responses to freeze-induced dehydration and subsequent rehydration are blocked, with resultant enhanced freezing injury.Microtubules are dynamic structures, existing in an equilibrium between soluble tubulin subunits and the polymerized filament. Several chemical compounds, ions, and environmental conditions shift the equilibrium toward depolymerization (8). Included in the list of destabilizing agents are freezing and low temperature.

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Lateral diffusion in the plasma membrane of maize protoplasts with implications for cell culture

Cited by 3 publications

References 0 publications

Effects of Yariv phenylglycosides onRosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation

Effects of Yariv phenylglycosides onRosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation

Cold stability of microtubules in wood-forming tissues of conifers during seasons of active and dormant cambium

Effect of Microtubule Stabilization on the Freezing Tolerance of Mesophyll Cells of Spinach

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