Lateral flow immunoassay for 5-hydroxyflunixin based on near-infrared fluorescence molecule as an alternative label to gold nanoparticles

Fan, Ruiqi; Zhang, Wanjun; Jin, Yongpeng; Zhao, Rongmao; Yang, Chunjiang; Chen, Qian; He, Lin; Chen, Yiqiang

doi:10.1007/s00604-020-04338-z

Cited by 17 publications

(8 citation statements)

References 23 publications

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“…A detector in the device senses the light that the dye or the marker emits. Correct sensing is very important to quantify the target molecule as the amount of the target analyte in the sample immediately correlates with the produced light intensity [17][18][19]. In some cases, the target analyte concentration in the sample is estimated by comparing the intensity of the fluorescent signal to a reference signal [20].…”

Section: Working Principlementioning

confidence: 99%

“…The light source plays a critical role in providing the required energy for light emission by the target molecule excited in the sample kept within the lateral flow immunoassay test strip. Common light sources are light-emitting diodes [18,[45][46][47] and laser diodes [48,49]. A light-emitting diode (LED) is a solidstate, stable, and compact source producing light when a current is applied.…”

Section: Light Sourcementioning

confidence: 99%

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Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

AKSOY

DULDA

Ertas

2023

EJOSAT

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Fluorescence-based optical test strip readers are used to detect and quantify fluorescent signals from immunoassay test strips in medicine, especially for point-of-care applications. The design of optical systems including light sources and detection systems in these devices is not only indispensable but also the most critical part for specific detection applications. This study aims to provide detailed information about fluorescence-based optical test strip readers, existing and emerging technologies, and their contributions to the design of the device. The most commonly used technologies of light sources and detection systems have been discussed and compared for the ideal design. Arc and Xenon lamps may not be appropriate for portable and low-cost devices as they are larger and more costly when compared to LEDs and laser diodes. Photodiodes and CMOS detectors can be used for the design of low-cost, portable fluorescence-based optical test strip readers as they are cheaper and smaller in size when compared to CCDs and PMTs. Both light source and detector should be chosen according to the application priorities and spectral characteristics of the fluorescent molecule by integrating them with proper optical elements like filters, mirrors, etc. This study contributes to the people who are interested in the design of fluorescence-based optical test strip readers as it serves as a guideline for the optical test strip reader systems.

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Section: Working Principlementioning

confidence: 99%

Section: Light Sourcementioning

confidence: 99%

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

AKSOY

DULDA

Ertas

2023

EJOSAT

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“…14 By using 5-FLU as a hapten coupled to bovine serum albumin (BSA) to prepare an immunogen, Fan et al obtained a monoclonal antibody (mAb) with an IC 50 of 3.2 ng mL −1 for 5-FLU and 3.3 ng mL −1 for FLU. 1 Although this mAb had a broad specificity, it had low affinity for both FLU and 5-FLU. We can conclude that the antibodies prepared in these studies demonstrate either low affinity or unclear broad specificity recognition to both FLU and 5-FLU.…”

Section: Introductionmentioning

confidence: 99%

Antibody Production, Immunoassay Establishment, and Specificity Study for Flunixin and 5-Hydroxyflunixin

Li,

Mi,

Bai

et al. 2024

J. Agric. Food Chem.

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Flunixin (FLU) is a nonsteroidal drug that is widely used in animals, causing severe drug residues in animal-derived foods and environment. The development of antibody-based rapid immunoassay methods is of great significance for the monitoring of FLU and its metabolite 5-hydroxyflunixin (5-FLU). We prepared monoclonal antibodies (mAbs) with different recognition spectra through FLU-keyhole limpet hemocyanin conjugates as immunogen coupled with antibody screening strategies. mAb5E6 and mAb6D7 recognized FLU with high affinity, and mAb2H5 and mAb4A4 recognized FLU and 5-FLU with broad specificity. Through evaluating the recognition of these mAbs against more than 11 structural analogues and employing computational chemistry, molecular docking, and molecular dynamics methodologies, we preliminarily determined the recognition epitope and recognition mechanism of these mAbs. Finally, an indirect competitive enzyme-linked immunosorbent assay for FLU based on mAb6D7 was developed, which exhibited limits of detection as low as 0.016−0.042 μg kg −1 (L −1 ) in milk and muscle samples.

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“…Nevertheless, it was reported to be limited by two major aspects: low sensitivity and inability to analyze quantitatively (Bahadir and Sezginturk, 2016). Thus, numerous methods have been developed to solve the problems such as fluorescence, surface plasmon resonance, and chemiluminescence (Cui et al, 2015;Fan et al, 2020;Kim K et al, 2021). However, these developed methods have not satisfied the detection limit and precision applicable to clinical practice so far.…”

Section: Introductionmentioning

confidence: 99%

SERS Based Lateral Flow Assay for Rapid and Ultrasensitive Quantification of Dual Laryngeal Squamous Cell Carcinoma-Related miRNA Biomarkers in Human Serum Using Pd-Au Core-Shell Nanorods and Catalytic Hairpin Assembly

Niu

et al. 2022

Front. Mol. Biosci.

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Non-invasive early diagnosis is of great significant in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. However, it was still a challenge to report an accurate and sensitive method for the detection of miRNA during cancer development, especially in the presence of its analogs that produce intense background noise. Herein, we developed a surface-enhanced Raman scattering (SERS)–based lateral flow assay (LFA) biosensor, assisted with catalytic hairpin assembly (CHA) amplification strategy, for the dynamic monitoring of miR-106b and miR-196b, associated with laryngeal squamous cell carcinoma (LSCC). In the presence of target miRNAs, two hairpin DNAs could self-assemble into double-stranded DNA, exposing the biotin molecules modified on the surface of palladium (Pd)–gold (Au) core–shell nanorods (Pd-AuNRs). Then, the biotin molecules could be captured by the streptavidin (SA), which was fixed on the test lines (T1 line and T2 line) beforehand. The core–shell spatial structures and aggregation Pd-AuNRs generated abundant active “hot spots” on the T line, significantly amplifying the SERS signals. Using this strategy, the limits of detections were low to aM level, and the selectivity, reproducibility, and uniformity of the proposed SERS-LFA biosensor were satisfactory. Finally, this rapid analysis strategy was successfully applied to quantitatively detect the target miRNAs in clinical serum obtained from healthy subjects and patients with LSCC at different stages. The results were consistent with the quantitative real-time PCR (qRT-PCR). Thus, the CHA-assisted SERS-LFA biosensor would become a promising alternative tool for miRNAs detection, which showed a tremendous clinical application prospect in diagnosing LSCC.

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Lateral flow immunoassay for 5-hydroxyflunixin based on near-infrared fluorescence molecule as an alternative label to gold nanoparticles

Cited by 17 publications

References 23 publications

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

Antibody Production, Immunoassay Establishment, and Specificity Study for Flunixin and 5-Hydroxyflunixin

SERS Based Lateral Flow Assay for Rapid and Ultrasensitive Quantification of Dual Laryngeal Squamous Cell Carcinoma-Related miRNA Biomarkers in Human Serum Using Pd-Au Core-Shell Nanorods and Catalytic Hairpin Assembly

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