Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity

Fang, Zhiyuan; Huang, Jing; Lie, Puchang; Xiao, Zhuo; Ouyang, Chuanyan; Wu, Qing; Wu, Yibo; Liu, Guodong; Zeng, Lingwen

doi:10.1039/c0cc02782k

Cited by 109 publications

(46 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Liu et al, 2014;C. Liu et al, 2014) and metallic cations (Mazumdar et al, 2010;Torabi and Lu, 2011;Kuang et al, 2013;López-Marzo et al, 2013aFang et al, 2010) also can be detected using several strategies. In the case of the work of Fang et al (2010), Cu 2 þ ions can be detected taking advantage of the cleavage that cooper causes, in presence of ascorbate, on Cu 2 þ -specific-DNAzymes (linked to AuNPs) which, if broken, will be captured on TL.…”

Section: Designed a Lfsmentioning

confidence: 97%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

View full text Add to dashboard Cite

Lateral flow biosensors (LFBs) are paper-based devices which permit the performance of low-cost and fast diagnostics with good robustness, specificity, sensitivity and low limits of detection. The use of nanoparticles (NPs) as labels play an important role in the design and fabrication of a lateral flow strip (LFS). The choice of NPs and the corresponding detection method directly affect the performance of these devices. This review discusses aspects related to the application of different nanomaterials (e.g. gold nanoparticles, carbon nanotubes, quantum dots, up-converting phosphor technologies, and latex beads, between others) in LFBs. Moreover, different detection methods (colorimetric, fluorescent, electrochemical, magnetic, etc.) and signal enhancement strategies (affording secondary reactions or modifying the architecture of the LFS) as well as the use of devices such as smartphones to mediate the response of LFSs will be analyzed.

show abstract

Section: Designed a Lfsmentioning

confidence: 97%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

View full text Add to dashboard Cite

show abstract

“…Zeng and coworkers utilized Cu 2+ - and Pb 2+ -dependent DNAzymes to fabricate a similar lateral flow device for the detection of Cu 2+ (Figure 8B) and Pb 2+ , respectively. 158,159 The introduction of a catalytic DNA circuit in the Pb 2+ -sensitive device dramatically enhanced the sensitivity of the sensor when compared with previous solution-based approaches. 159 …”

Section: Metal Ion-dependent Dnazymes For Metal Ion Recognitionmentioning

confidence: 99%

DNA as Sensors and Imaging Agents for Metal Ions

Xiang

Lu²

2013

Inorg. Chem.

142

104

View full text Add to dashboard Cite

Increasing interests in detecting metal ions in many chemical and biomedical fields have created demands for developing sensors and imaging agents for metal ions with high sensitivity and selectivity. This review covers recent progress in DNA-based sensors and imaging agents for metal ions. Through both combinatorial selection and rational design, a number of metal ion-dependent DNAzymes and metal ion-binding DNA structures that can selectively recognize specific metal ions have been obtained. By attaching these DNA molecules with signal reporters such as fluorophores, chromophores, electrochemical tags, and Raman tags, a number of DNA-based sensors for both diamagnetic and paramagnetic metal ions have been developed for fluorescent, colorimetric, electrochemical, and surface Raman detections. These sensors are highly sensitive (with detection limit down to 11 ppt) and selective (with selectivity up to millions-fold) toward specific metal ions. In addition, through further development to simplify the operation, such as the use of “dipstick tests”, portable fluorometers, computer-readable discs, and widely available glucose meters, these sensors have been applied for on-site and real-time environmental monitoring and point-of-care medical diagnostics. The use of these sensors for in situ cellular imaging has also been reported. The generality of the combinatorial selection to obtain DNAzymes for almost any metal ion in any oxidation state, and the ease of modification of the DNA with different signal reporters make DNA an emerging and promising class of molecules for metal ion sensing and imaging in many fields of applications.

show abstract

“…In the absence of Cu 2+ , however, no release of the ssDNA occurred, thus the AuNPs moved across the test line and were captured on the control line where complementary DNA was immobilized. Therefore, a red line appeared on the test line only when Cu 2+ was present in the samples [255]. Later, the same research group further incorporated a catalytic DNA circuit that could amplify the release of a ssDNA by the 8-17 DNAzyme and its substrate, achieving the detection of Pb 2+ on a lateral-flow device with high sensitivity [277].…”

Section: Nucleic Acid Cleaving/ligating Dnazymes and Aunps For Biosenmentioning

confidence: 98%

DNAzyme-Functionalized Gold Nanoparticles for Biosensing

Xiang

Tan

et al. 2013

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

Recent progress in using DNAzyme-functionalized gold nanoparticles (AuNPs) for biosensing is summarized in this chapter. A variety of methods, including those for attaching DNA on AuNPs, detecting metal ions and small molecules by DNAzyme-functionalized AuNPs, and intracellular applications of DNAzyme-functionalized AuNPs are discussed. DNAzyme-functionalized AuNPs will increasingly play more important roles in biosensing and many other multidisciplinary applications. This chapter covers the recent advancement in biosensing applications of DNAzyme-functionalized gold nanoparticles, including the detection of metal ions, small molecules, and intracellular imaging.

show abstract

Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity

Abstract: A lateral flow nucleic acid biosensor based on copper-dependent DNA-cleaving DNAzyme and gold nanoparticles has been developed for the visual detection of copper ions (Cu(2+)) in an aqueous solution with a detection limit of 10 nM.

Cited by 109 publications

References 22 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

DNA as Sensors and Imaging Agents for Metal Ions

DNAzyme-Functionalized Gold Nanoparticles for Biosensing

Contact Info

Product

Resources

About