Latex agglutination test for detection of Clostridium difficile toxin in stool samples

Shahrabadi, M. S.; Bryan, L. E.; Gaffney, Donna; Coderre, S.; Gordon, Ralph C.; Pai, Chik Hyun

doi:10.1128/jcm.20.3.339-341.1984

Cited by 38 publications

(7 citation statements)

References 21 publications

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“…Sequences of the primers and probes used in the PCR P146 GCT GGC TAA GAT TCT ATA TTT TTG TCC AGT a 868-897 c EntA GAA CGC CAA TCA TAT AAA TTT CCA GCT GGG a 783-812 c a Complementary DNA strand. Position in the C. perfringens phospholipase C gene sequence(25). Position in the CPE gene sequence(28).…”

mentioning

confidence: 99%

Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test

Fach

Popoff

1997

Appl Environ Microbiol

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A duplex PCR procedure was evaluated for the detection of Clostridium perfringens in food and biological samples and for the identification of enterotoxigenic strains. This method uses two sets of primers which amplify in the same reaction two different DNA fragments simultaneously: the 283-bp C. perfringens phospholipase C gene fragment and the 426-bp enterotoxin gene fragment. Internal primers within the two primer sets confirmed the specificity of the method by DNA-DNA hybridization with the PCR products. No cross-reaction was observed with other Clostridium species or with other bacteria routinely found in food. The detection level was approximately 10 5 C. perfringens cells per g of stool or food sample. When overnight enrichment culture was used, 10 C. perfringens cells per g was detected in 57 artificially contaminated food samples. The duplex PCR is a rapid, sensitive, and reliable method for the detection and identification of enterotoxigenic C. perfringens strains in food samples. A slide latex agglutination test was also evaluated as a rapid, simple technique for the detection of C. perfringens enterotoxin in stool samples.

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mentioning

confidence: 99%

Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test

Fach

Popoff

1997

Appl Environ Microbiol

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“…There is only one report on the use of antibody-latex agglutination test in detection of C. difficile , in which positive latex results were confirmed by cytotoxicity assay. Accordingly, this test can be an acceptable diagnostic tool for screening C. difficile toxins ( Shahrabadi et al, 1984 ), which can be considered a simple and affordable diagnostic tool for clinical use.…”

Section: Development Of Rapid Detection Methods Based On Recombinant ...mentioning

confidence: 99%

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives

et al. 2022

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Clostridioides difficile, the most common cause of nosocomial diarrhea, has been continuously reported as a worldwide problem in healthcare settings. Additionally, the emergence of hypervirulent strains of C. difficile has always been a critical concern and led to continuous efforts to develop more accurate diagnostic methods for detection of this recalcitrant pathogen. Currently, the diagnosis of C. difficile infection (CDI) is based on clinical manifestations and laboratory tests for detecting the bacterium and/or its toxins, which exhibit varied sensitivity and specificity. In this regard, development of rapid diagnostic techniques based on antibodies has demonstrated promising results in both research and clinical environments. Recently, application of recombinant antibody (rAb) technologies like phage display has provided a faster and more cost-effective approach for antibody production. The application of rAbs for developing ultrasensitive diagnostic tools ranging from immunoassays to immunosensors, has allowed the researchers to introduce new platforms with high sensitivity and specificity. Additionally, DNA encoding antibodies are directly accessible in these approaches, which enables the application of antibody engineering to increase their sensitivity and specificity. Here, we review the latest studies about the antibody-based ultrasensitive diagnostic platforms for detection of C. difficile bacteria, with an emphasis on rAb technologies.

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“…The gold standard for detecting toxin is a tissue culture cytotoxicity assay. 8 Other techniques, including enzyme immunosorbent assay (EIA), 9 c o u n t e r i m m u n o e l e c t r o p h o r e s i s (CIE), 10 and latex agglutination 11 have been proposed to supplant this technique. However, EIA reagents are not commercially available, CIE lacks sensitivity and specificity, and the latex agglutination test which is commercially available is currently clouded in controversy.…”

Section: Diarrheal Disease In the Hospitalized Patientmentioning

confidence: 99%

Diarrheal Disease in the Hospitalized Patient

Gilligan

1986

Infect. Control

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In the past 15 years, our knowledge of the pathogenesis and etiologic agents of diarrheal disease has expanded rapidly. No longer is it scientifically appropriate or cost effective for the laboratory simply to culture a stool for Salmonella and Shigella and to do a wet mount for amebae. Today we have a plethora of agents including, but not limited to, Campylobacter, Yersinia, Clostridium difficile, Escherichia coli, Aeromonas, Plesiomonas, vibrios, rotavirus, Norwalk agent, Giardia, and Cryptosporidium, which have all been suggested to be agents of diarrheal disease. Given such a wide range of possibilities, what approaches should be taken to determine the etiologic agent of diarrheal disease in the hospitalized patient in an era of cost containment?

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Latex agglutination test for detection of Clostridium difficile toxin in stool samples

Cited by 38 publications

References 21 publications

Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test

Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives

Diarrheal Disease in the Hospitalized Patient

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