Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties

Zhang, Yanmei; Wang, Fumiao; Huang, Qiaoling; Patil, Aniruddha; Hu, Juncheng; Fan, Lili; Yang, Yun Jung; Duan, Huajun; Dong, Xiang; Lin, Changjian

doi:10.1016/j.msec.2020.110690

Cited by 32 publications

(20 citation statements)

References 56 publications

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“…The antibacterial characteristics of ε-polylysine is well established in food industry and it is increasingly being applied in biomedicine in recent years [ 19 , 20 , 21 , 22 ]. Few studies have utilized ε-polylysine for dental application purposes, including composite [ 23 , 24 ], dental adhesive [ 25 ], implant surface modification [ 26 ] and antimicrobials [ 27 , 28 ]. However, the effect of ε-polylysine on P. gingivalis is less studied and the investigating methods of its antibacterial effectiveness against oral microorganisms were limited primarily to inhibition of planktonic bacterial growth.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries

et al. 2020

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Antimicrobials are important adjuncts in the treatment of caries and periodontitis. However, increased bacterial resistance and hypersensitivity reactions to commonly used antimicrobials have led to an increasing demand for safe and natural substances. The objective of this study was to investigate the antibacterial effects of ε-polylysine against oral pathogens Streptococcus mutans and Porphyromonas gingivalis. Broth dilution assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) analyses were performed to explore the antibacterial effect of ε-polylysine against S. mutans strain ATCC25175 and P. gingivalis strain ATCC332277. For the test solution, ε-polylysine was added to the bacterial suspension to prepare 0.125%, 0.25%, 0.5% and 1% ε-polylysine solutions diluted in broth medium. All four concentrations demonstrated complete inhibition of S. mutans and significantly reduced viable cell counts of P. gingivalis after 24 h. From starting inoculum of 9.15 log CFU/mL, P. gingivalis cell counts reduced to 4.01 log CFU/mL in the 0.125% ε-polylysine treatment group. SEM, CLSM, and the LIVE/DEAD bacterial assay of ε-polylysine application on P. gingivalis biofilm-dentin specimens revealed bacterial cell membrane disruption and irregular cell morphologies. The results indicated satisfactory antibacterial efficacy of ε-polylysine against P. gingivalis and S. mutans in liquid medium and as an application on biofilm-dentin specimens.

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Section: Introductionmentioning

confidence: 99%

Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries

et al. 2020

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“…The remaining studies of carried medicines involved polydopamine (89,90), stem cells, microRNA nanoparticles (91), plasmid deoxyribonucleic acid (DNA) (92), and other drugs that meet the different specific design requirements (93,94).…”

Section: Coating Technologymentioning

confidence: 99%

Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review

Zuo

Lin

et al. 2021

Ann Transl Med

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Owing to their excellent biocompatibility and corrosion-resistant properties, titanium (Ti) (and its alloy) are essential artificial substitute biomaterials for orthopedics. However, flaws, such as weak osteogenic induction ability and higher Young's modulus, have been observed during clinical application. As a result, short-and long-term postoperative follow-up has found that several complications have occurred.For decades, scientists have exerted efforts to compensate for these deficiencies. Different modification methods have been investigated, including changing alloy contents, surface structure transformation, threedimensional (3D) structure transformation, coating, and surface functionalization technologies. The cellsurface interaction effect and imitation of the natural 3D bone structure are the two main mechanisms of these improved methods. In recent years, significant progress has been made in materials science research methods, including thorough research of titanium alloys of different compositions, precise surface pattern control technology, controllable 3D structure construction technology, improvement of coating technologies, and novel concepts of surface functionalization. These improvements facilitate the possibility for further research in the field of bone tissue engineering. Although the underlying mechanism is still not fully understood, these studies still have some implications for clinical practice. Therefore, for the direction of further research, it is beneficial to summarize these studies according to the basal method used. This literature review aimed to classify these technologies, thereby providing beginners with a preliminary understanding of the field.

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“…Zhang et al coated an array of titanium dioxide nanotubes with a multilayer assembled with the antimicrobial peptide ε-polylysine as polication and gum Arabic (polyanion) [ 115 ]. The anodized titanium surface was initially functionalized with polydopamine in order to covalently graft the first layer of ε-polylysine.…”

Section: Lbl For Antimicrobial Peptides Encapsulationmentioning

confidence: 99%

“…( F ) Microscopy images of rat bone marrow mesenchymal stem cells stained for actyn cytoskeleton and cell nucleus 4 days after seeding on the osteogenic layer. A, B and C adapted with permission from [ 115 ]. Elsevier, 2020.…”

Section: Figurementioning

confidence: 99%

Antibacterial Layer-by-Layer Coatings for Medical Implants

2020

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The widespread occurrence of nosocomial infections and the emergence of new bacterial strands calls for the development of antibacterial coatings with localized antibacterial action that are capable of facing the challenges posed by increasing bacterial resistance to antibiotics. The Layer-by-Layer (LbL) technique, based on the alternating assembly of oppositely charged polyelectrolytes, can be applied for the non-covalent modification of multiple substrates, including medical implants. Polyelectrolyte multilayers fabricated by the LbL technique have been extensively researched for the development of antibacterial coatings as they can be loaded with antibiotics, antibacterial peptides, nanoparticles with bactericide action, in addition to being capable of restricting adhesion of bacteria to surfaces. In this review, the different approaches that apply LbL for antibacterial coatings, emphasizing those that can be applied for implant modification are presented.

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Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties

Cited by 32 publications

References 56 publications

Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries

Antibacterial Effect of the Natural Polymer ε-Polylysine Against Oral Pathogens Associated with Periodontitis and Caries

Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review

Antibacterial Layer-by-Layer Coatings for Medical Implants

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