Antibacterial Layer-by-Layer Coatings for Medical Implants

Escobar, Ane; Muzzio, Nicolás Eduardo; Moya, Sergio

doi:10.3390/pharmaceutics13010016

Cited by 57 publications

(53 citation statements)

References 152 publications

(196 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The binding of polyelectrolytes to cells occurs due to the electrostatic interaction with a negatively charged plasma membrane [16,17]. One of the possible applications for polyelectrolyte multilayers would be to improve the bio-interface of implantable materials [18]. Thus, these polymers need to be biocompatible, nontoxic, non-immunogenic, biodegradable, have a high drug-carrying capacity, and have controlled release of the drugs at the target site.…”

Section: Introductionmentioning

confidence: 99%

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

et al. 2021

View full text Add to dashboard Cite

Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems.

show abstract

Section: Introductionmentioning

confidence: 99%

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The magnetron-sputtering (MS) method involves the sputtering of metallic targets and the deposition of nanometric clusters on a substrate located a few centimetres below the target. The method is very useful for incorporating ions on the surface of titanium implants [ 32 , 33 , 34 ]. The deposition of thin films over metals and alloys is also straightforward.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Antibacterial Metal Surfaces Using Magnetron-Sputtering Method

et al. 2021

View full text Add to dashboard Cite

One-hundred-nanometer films consisting of silver, copper, and gold nanocrystallites were prepared, and their antibacterial properties were quantitatively measured. The magnetron-sputtering method was used for the preparation of the metallic films over the glass plate. Single- and double-layer films were manufactured. The films were thoroughly characterized with the XRD, SEM, EDS, and XPS methods. The antibacterial activity of the samples was investigated. Gram-negative Escherichia coli, strain K12 ATCC 25922 (E. coli), and Gram-positive Staphylococcus epidermidis, ATCC 49461 (S. epidermidis), were used in the microbial tests. The crystallite size was about 30 nm in the cases of silver and gold and a few nanometers in the case of copper. Significant oxidation of the copper films was proven. The antibacterial efficacy of the tested samples followed the order: Ag/Cu > Au/Cu > Cu. It was concluded that such metallic surfaces may be applied as contact-killing materials for a more effective fight against bacteria and viruses.

show abstract

“…Studies have proposed approaches to enhance implant osseointegration by developing an osteogenic surface or to prevent infection by incorporating antibiotic molecules or peptides in the surface coating (Smeets et al 2016; Escobar et al 2021). One promising alternative could be the development of implants with multifunctional surfaces capable of interacting with the host tissue to increase osseointegration while reducing bacterial activity (Raphel et al 2016; Rao et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

et al. 2021

View full text Add to dashboard Cite

Osseointegration of dental, craniofacial, and orthopedic implants is critical for their long-term success. Multifunctional surface treatment of implants was found to significantly improve cell adhesion and induce osteogenic differentiation of dental-derived stem cells in vitro. Moreover, local and sustained release of antibiotics via nanolayers from the surface of implants can present unparalleled therapeutic benefits in implant dentistry. Here, we present a layer-by-layer surface treatment of titanium implants capable of incorporating BMP-2–mimicking short peptides and gentamicin to improve their osseointegration and antibacterial features. Additionally, instead of conventional surface treatments, we employed polydopamine coating before layer-by-layer assembly to initiate the formation of the nanolayers on rough titanium surfaces. Cytocompatibility analysis demonstrated that modifying the titanium implant surface with layer-by-layer assembly did not have adverse effects on cellular viability. The implemented nanoscale coating provided sustained release of osteoinductive peptides with an antibacterial drug. The surface-functionalized implants showed successful osteogenic differentiation of periodontal ligament stem cells and antimicrobial activity in vitro and increased osseointegration in a rodent animal model 4 wk postsurgery as compared with untreated implants. Altogether, our in vitro and in vivo studies suggest that this approach can be extended to other dental and orthopedic implants since this surface functionalization showed improved osseointegration and an enhanced success rate.

show abstract

Antibacterial Layer-by-Layer Coatings for Medical Implants

Cited by 57 publications

References 152 publications

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Fabrication of Antibacterial Metal Surfaces Using Magnetron-Sputtering Method

Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

Contact Info

Product

Resources

About