Layer-by-layer modification strategies for electrochemical detection of biomarkers

Erkmen, Cem; Selcuk, Ozge; Ünal, Didem Nur; Kurbanoğlu, Sevinç; Uslu, Bengi

doi:10.1016/j.biosx.2022.100270

Cited by 6 publications

(5 citation statements)

References 102 publications

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“…Recently, Gajos et al in 2023, reported a non-covalent inflow biofunctionalization for capture assays for IgG antibodies. One of the most typical layer-by-layer techniques for self-assembly for various biomolecular immobilization [61]. Caruso et al introduced the study of a polyamine hydrochloride/polystyrene sulphonate layer for SAM mercaptopropionic acid, enabling a charged modification on the transducer element [62].…”

Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Gandhi

2024

Electrochem

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Electrochemistry is a hotspot in today’s research arena. Many different domains have been extended for their role towards the Internet of Things, digital health, personalized nutrition, and/or wellness using electrochemistry. These advances have led to a substantial increase in the power and popularity of electroanalysis and its expansion into new phases and environments. The recent COVID-19 pandemic, which turned our lives upside down, has helped us to understand the need for miniaturized electrochemical diagnostic platforms. It also accelerated the role of mobile and wearable, implantable sensors as telehealth systems. The major principle behind these platforms is the role of electrochemical immunoassays, which help in overshadowing the classical gold standard methods (reverse transcriptase polymerase chain reaction) in terms of accuracy, time, manpower, and, most importantly, economics. Many research groups have endeavoured to use electrochemical and bio-electrochemical tools to overcome the limitations of classical assays (in terms of accuracy, accessibility, portability, and response time). This review mainly focuses on the electrochemical technologies used for immunosensing platforms, their fabrication requirements, mechanistic objectives, electrochemical techniques involved, and their subsequent output signal amplifications using a tagged and non-tagged system. The combination of various techniques (optical spectroscopy, Raman scattering, column chromatography, HPLC, and X-ray diffraction) has enabled the construction of high-performance electrodes. Later in the review, these combinations and their utilization will be explained in terms of their mechanistic platform along with chemical bonding and their role in signal output in the later part of article. Furthermore, the market study in terms of real prototypes will be elaborately discussed.

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Section: Functional Model Of Bonding Between the Protein And The Unde...mentioning

confidence: 99%

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Gandhi

2024

Electrochem

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“…For decades, layer-by-layer (LBL) self-assembly for constructing nano-films has brought hope to solve this problem [22,23]. The principle of LBL technology is to use strong interaction forces (such as chemical bonds, etc.)…”

Section: Introductionmentioning

confidence: 99%

The layer-by-layer assembled ERGO+/ERGO− multilayer modified electrode for sensitive detection of dopamine

Lin,

Mo,

Dai

et al. 2024

Mater. Res. Express

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Graphene materials represented by graphene oxide (GO) have been widely regarded as functional coatings or films to modify surface of the electrode for detecting dopamine molecules. However, interfacial material properties for detection sensitivity, film stability, and applicability to electrodes are still highly desired. Herein, we first present a screen-printing carbon electrode (SPCE) coated with an electrochemically reduced layer-by-layer (LbL) assembled multilayer driven by an electrostatic interaction between positively charged polyethyleneimine-modified GO with amine groups (ERGO+) and negatively charged carboxyl-functionalized GO (ERGO-), which is briefly described as (ERGO+/ERGO-)n/SPCE. Firstly, without using conventional glassy carbon and gold electrodes, SPCE was tried to make coatings adapt to more flexible and unstable electrodes, simultaneously guaranteeing higher detection performance. Secondly, although a variety of electrochemical sensors such as GO-/SPCE and ERGO-/SPCE were obtained through the drop-casting technique, as-prepared (ERGO+/ERGO-)n/SPCE showed much higher electrocatalytic activities with enhanced peak current signals and reduced charge transfer resistance. Finally, excellent electrochemical properties and sensing performances of the (ERGO+/ERGO-)n/SPCE sensor for detection of dopamine were demonstrated, especially having a linear range of 1 μM to 1000 μM. Meanwhile, the detection limit is as low as 0.39 μM and S/N is equal to 3. The present work offers a potential direction to develop GO modified electrodes for sensitive biomolecular detection.

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“…Since its conception in the 1950s, researchers have been interested in studying the fundamentals of the effects of shape, surrounding medium, material, and their interaction with light of different wavelengths [ 1 ]. With this well-established knowledge, plasmonics is witnessing an enormous potential for applications in different fields, including forensics [ 2 ]; environmental safety [ 3 ]; biosensing [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ], e.g., SARS-CoV-2 detection [ 12 ]; and homeland security [ 13 ]. The applications of plasmonics majorly rely on surface plasmon resonance (SPR) or localized surface plasmon resonance (LSPR) effects [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Trends in SERS-Based Plasmonic Sensors for Disease Diagnostics, Biomolecules Detection, and Machine Learning Techniques

2023

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Surface-enhanced Raman spectroscopy/scattering (SERS) has evolved into a popular tool for applications in biology and medicine owing to its ease-of-use, non-destructive, and label-free approach. Advances in plasmonics and instrumentation have enabled the realization of SERS’s full potential for the trace detection of biomolecules, disease diagnostics, and monitoring. We provide a brief review on the recent developments in the SERS technique for biosensing applications, with a particular focus on machine learning techniques used for the same. Initially, the article discusses the need for plasmonic sensors in biology and the advantage of SERS over existing techniques. In the later sections, the applications are organized as SERS-based biosensing for disease diagnosis focusing on cancer identification and respiratory diseases, including the recent SARS-CoV-2 detection. We then discuss progress in sensing microorganisms, such as bacteria, with a particular focus on plasmonic sensors for detecting biohazardous materials in view of homeland security. At the end of the article, we focus on machine learning techniques for the (a) identification, (b) classification, and (c) quantification in SERS for biology applications. The review covers the work from 2010 onwards, and the language is simplified to suit the needs of the interdisciplinary audience.

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Layer-by-layer modification strategies for electrochemical detection of biomarkers

Cited by 6 publications

References 102 publications

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

Modelling Prospects of Bio-Electrochemical Immunosensing Platforms

The layer-by-layer assembled ERGO+/ERGO− multilayer modified electrode for sensitive detection of dopamine

Recent Trends in SERS-Based Plasmonic Sensors for Disease Diagnostics, Biomolecules Detection, and Machine Learning Techniques

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