LB Film Containing Acetylcholinesterase for Fiber-Optic Organophosphorus Sensor

Biomedical application of immobilized enzymes

“…In addition, increased sensitivity and selectivity of a enzyme‐based electrode can also be obtained by modifying the electrode surface with semiconducting films using electropolymerization of a redox monomer, such as phenazine,53 phenoxazine and phenothiazine derivatives,54 viologens,55 and thionine 56. Polymer‐modified electrodes with a three‐dimensional distribution of mediators are preferred over a monolayer distribution of mediators for the design of biosensors because a much larger catalytic response of the polymer coating will be obtained because of the volume effect.…”

“…In addition to minimizing oxygen sensitivity, coupled mediated sensing systems, in which the mediator is attached to either the electrode or to the enzyme, could also provide a faster electron transfer, improved biosensor lifetime, 51 and reducing susceptibility to interferences from other electrochemically active species because of the low potential generally needed for oxidation of the mediators. 52 In addition, increased sensitivity and selectivity of a enzyme-based electrode can also be obtained by modifying the electrode surface with semiconducting films using electropolymerization of a redox monomer, such as phenazine, 53 phenoxazine and phenothiazine derivatives, 54 viologens, 55 and thionine. 56 Polymer-modified electrodes with a three-dimensional distribution of mediators are preferred over a monolayer distribution of mediators for the design of biosensors because a much larger catalytic response of the polymer coating will be obtained because of the volume effect.…”