LbetaT2 gonadotroph cells secrete follicle stimulating hormone (FSH) in response to active A

Graham, Kathryn E.; Nusser, Kevin D.; Low, Malcolm J.

doi:10.1677/joe.0.162r001

Cited by 111 publications

(69 citation statements)

References 26 publications

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“…In fact, in the rat, activin bA-subunit-immunostained ®bers were distributed throughout the hypothalamus and GnRH-positive perikarya, and ®bers were in close association with bA subunit-immunoreactive ®bers (26). In addition, it was reported that FSH secretion is stimulated over 6-fold by concomitant GnRH and activin A administration (8). So, the presence of activin A in those cells containing and secreting GnRH (FNC-B4) suggests that these peptides may regulate the reproductive function modulating pituitary FSH release and biosynthesis; GnRH stimulation of activin peptide production provides regulatory control over the production of FSH.…”

Section: Discussionmentioning

confidence: 99%

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Human GnRH-secreting cultured neurons express activin betaA subunit mRNA and secrete dimeric activin A

Florio

Vannelli²,

Luisi³

et al. 2000

European Journal of Endocrinology

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Objective: To evaluate the expression of activin bA-subunit mRNA and the secretion of activin A in/from cultured GnRH-secreting neuronal cells cloned from human olfactory epithelium (FNC-B4), which showed biochemical and antigenic properties of GnRH-secreting neurons. Design: FNC-B4 cells were cultured in basal and conditioned media. Methods: Reverse transcription-polymerase chain reaction (RTR±PCR) evaluated the expression of activin bA-subunit mRNA. By using a speci®c ELISA, dimeric activin A concentrations were measured in culture media, in the absence or presence of carvone or forskolin and with different doses of progesterone, GnRH, and estradiol. Results: RT±PCR experiments performed on total RNA isolated from FNC-B4 cells, using speci®c primers for the activin bA gene, showed a 787 bp DNA band corresponding to the bA gene. FNC-B4 cells secreted activin A, and the highest accumulation in conditioned medium was achieved after 3 h culture: the addition of forskolin, but not of carvone, was able to stimulate the release of activin A from cultured neuronal cells (P < 0.01). When progesterone or GnRH was added, a signi®cant accumulation of activin A was observed (P < 0.01), while estradiol administration did not signi®cantly affect activin A secretion. Conclusion: To date, this is the only study, in an in vitro human model reporting, that GnRH-secreting neuronal cells expressed activin bA-subunit mRNA, and released dimeric activin A in culture medium. The expression and secretion of activin suggests that in these cells activin A might exert its action by autocrine/paracrine mechanisms.

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Section: Discussionmentioning

confidence: 99%

“…With regards to activin A action within the hypothalamo±pituitary±gonadal axis, the stimulatory role on FSH release from pituitary cells is well documented (1,7,8). In fact, activin induces a rapid and marked increases in FSH b-subunit mRNA and FSH release (1, 7).…”

Section: Introductionmentioning

confidence: 99%

Human GnRH-secreting cultured neurons express activin betaA subunit mRNA and secrete dimeric activin A

Florio

Vannelli²,

Luisi³

et al. 2000

European Journal of Endocrinology

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“…The ␣T1-1 cells represent a precursor to the gonadotrope/thyrotrope lineages as they express pituitary glycoprotein hormone ␣ subunit (CGA), whereas the ␣T3-1 cells represent a precursor to the gonadotrope lineage because they express CGA, the GnRH receptor, and SF1 but not LHB and FSHB. The L␤T2 cell line has many characteristics of a mature, differentiated gonadotrope (57,58). CV-1 cells, a monkey kidney cell line that does not express detectable levels of SF1 or PTX1 (59,60), were also used.…”

Section: ␤-Gal Staining Of Lhb-cre X Rosa26mentioning

confidence: 99%

FOXO1 Transcription Factor Inhibits Luteinizing Hormone β Gene Expression in Pituitary Gonadotrope Cells

Arriola

Mayo

Skarra

et al. 2012

Journal of Biological Chemistry

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Background: Insulin signaling regulates luteinizing hormone (LH) production in pituitary gonadotrope cells through unknown mechanisms. Results: FOXO1 phosphorylation and cellular localization are regulated by insulin signaling, and FOXO1 represses LH ␤-subunit (Lhb) transcription. Conclusion: Our study highlights a novel mechanism for regulation of Lhb gene expression. Significance: FOXO1 may act as a metabolic sensor in controlling LH levels and fertility.

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“…ARIP2b cDNA was introduced into L T2 cells using TransFast liposome reagents. Stimulation by 50 ng/ml activin A and measurement of FSH levels in conditioned media by radioimmunoassay (RIA) were performed as previously described (Graham et al 1999). The sensitivity of the RIA was 4 ng/ml.…”

Section: Radioimmunoassay For Follicle Stimulating Hormone (Fsh)mentioning

confidence: 99%

“…4A). We also studied the effects of ARIP2b on FSH secretion in gonadotroph L T2 cells, which secrete FSH in response to activin (Graham et al 1999). L T2 cells were transfected with ARIP2b and CAGA-lux DNAs, and activin-induced luciferase activity was measured.…”

Section: Arip2b/2c Has a Stimulatory Effect On Activin-induced Signalmentioning

confidence: 99%

Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling

Liu¹,

Tsuchida²,

Matsuzaki³

et al. 2006

Journal of Endocrinology

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Activin type II receptors (ActRIIs) including ActRIIA and ActRIIB are serine/threonine kinase receptors that form complexes with type I receptors to transmit intracellular signaling of activins, nodal, myostatin and a subset of bone morphogenetic proteins. ActRIIs are unique among serine/threonine kinase receptors in that they associate with proteins having PSD-95, Discs large and ZO-1 (PDZ) domains. In our previous studies, we reported specific interactions of ActRIIs with two independent PDZ proteins named activin receptor-interacting proteins 1 and 2 (ARIP1 and ARIP2). Overexpression of both ARIP1 and ARIP2 reduce activin-induced transcription. Here, we report the isolation of two isoforms of ARIP2 named ARIP2b and 2c. ARIP2, ARIP2b and ARIP2c recognize COOH-terminal residues of ActRIIA that match a PDZ-binding consensus motif. ARIP2 and its isoforms have one PDZ domain in the NH 2 -terminal region, and interact with ActRIIA. Although PDZ domains containing GLGF motifs of ARIP2b and 2c are identical to that of ARIP2, their COOH-terminal sequences differ from that of ARIP2. Interestingly, unlike ARIP2, overexpression of ARIP2b or 2c did not affect ActRIIA internalization. ARIP2b/2c inhibit inhibitory actions of ARIP2 on activin signaling. ARIP2 is widely distributed in mouse tissues. ARIP2b/2c is expressed in more restricted tissues such as heart, brain, kidneys and liver. Our results indicate that although both ARIP2 and ARIP2b/2c interact with activin receptors, they regulate ActRIIA function in a different manner.

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LbetaT2 gonadotroph cells secrete follicle stimulating hormone (FSH) in response to active A

Cited by 111 publications

References 26 publications

Human GnRH-secreting cultured neurons express activin betaA subunit mRNA and secrete dimeric activin A

Human GnRH-secreting cultured neurons express activin betaA subunit mRNA and secrete dimeric activin A

FOXO1 Transcription Factor Inhibits Luteinizing Hormone β Gene Expression in Pituitary Gonadotrope Cells

Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling

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