LC–ESI-MS/MS analysis of total oligomeric flavonoid fraction of Cyperus rotundus and its antioxidant, macromolecule damage protective and antihemolytic effects

Kandikattu, Hemanth Kumar; Rachitha, P.; Krupashree, K.; Jayashree, G.V.; Abhishek, Virat; Khanum, Farhath

doi:10.1016/j.pathophys.2015.07.001

Cited by 37 publications

(18 citation statements)

References 39 publications

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“…Previous studies demonstrated that piperoside isolated from P. retrofractum exhibited moderate peroxyl radical scavenging and reducing potential activity [ 32 ], while Terminalia citrina was found to display protective effects against oxidant-induced Heinz body formation [ 33 ], which contributes to anaemia. Reported active constituents, including total oligomeric flavonoid fractions, nootkatone, aristolone, solavetivone, and orientin obtained from Cyperus rotundus , have protective effects against lipid peroxidation, DNA damage, antihemolytic activity, and anticancer treatment for erythroleukaemia cells, which might be correlated with their high antioxidant activities [ 34 ]. Earlier experiments have also demonstrated that Alpinia galangal exerts in vitro antimelanogenesis activity [ 35 ] and in vivo antiamnesiac activity through its antioxidant properties [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand

Chanthasri

Puangkeaw

Kunworarath

et al. 2018

BMC Complement Altern Med

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BackgroundUses of polyherbal formulations have played a major role in traditional medicine. The present study is focused on the formulations used in traditional Thai folkloric medicine as tonics or bracers. Twenty documented polyherbal mixtures, used as nourishing tonics by the folk healers in Phatthalung and Songkhla provinces in southern Thailand, are targeted. Despite traditional health claims, there is no scientific evidence to support the utilization of polyherbal formulations.MethodsThe phenolic and flavonoid contents of the polyherbal formulations and a series of antioxidant tests were applied to measure their capability as preventive or chain-breaking antioxidants. In addition, the cytotoxic activity of effective formulations was assayed in Vero cells.ResultsNinety-eight plant species belonging to 45 families were used to prepare the tested formulation. The preliminary results revealed that water extracts of THP-R016 and THP-R019 contain a high level of total phenolic and flavonoid contents and exhibit remarkable antioxidant activities, as tested by DPPH, ABTS, and FRAP assays. The extract of THP-R019 also showed the strongest metal chelating activities, whereas THP-R016 extract possessed notable superoxide anion and peroxyl radical scavenging abilities.ConclusionsThe data provide evidence that the water extracts of folkloric polyherbal formulations, particularly THP-R016, are a potential source of natural antioxidants, which will be valuable in the pharmaceutical and nutraceutical industries. The free radical scavenging of THP-R016 may be due to the contribution of phenolic and flavonoid contents. Useful characteristics for the consumer, such as the phytochemical profiles of active ingredients, cellular based antioxidant properties and beneficial effects in vivo, are under further investigation.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2131-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand

Chanthasri

Puangkeaw

Kunworarath

et al. 2018

BMC Complement Altern Med

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“…effectively protected against erythrocyte lysis induced by chronic alcohol consumption and strengthens the resistance of red blood cells, thus testifying to a powerful anti-hemolytic activity. The lysis of the erythrocyte membrane has also been shown to be attenuated by many plant extracts such as Salvia verbenaca [ 42 ], Allium rotundum [ 43 ] and Cyperus rotundus [ 44 ] as well as by some pharmacological agents such as pyrrole carboxylic acids [ 45 ] and S-triazole-2-thiols [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Myrtle berries seeds aqueous extract abrogates chronic alcohol consumption-induced erythrocytes osmotic stability disturbance, haematological and biochemical toxicity

2018

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BackgroundThis study examined the effects of chronic alcohol consumption in the rat erythrocytes membrane as well as the involvement of reactive oxygen species and proinflammatory cytokines in its pathogenicity in rats and evaluated the ameliorating effects of myrtle berries seeds aqueous extract (MBSAE).MethodsFifty adult male Wistar rats were equally divided into five groups and treated daily for two months as follows: control, ethanol (3 g kg− 1 b.w., p.o.), and ethanol + MBSAE (25, 50 and 100 mg kg− 1, b.w., p.o.).ResultsExposure of rats to alcohol caused significant changes of some haematological parameters, enhanced erythrocytes hemolysis as well as an overproduction of reactive oxygen species such as H2O2, OH• radical and superoxide anion, hence the increase of lipoperoxidation and the depletion of antioxidant enzymes activity as well as non-enzymatic antioxidant (-SH groups and GSH) levels. On the other hand, ethanol intoxication caused the increase of serum TNFα, IL-8, IL-6 and 1Lβ, markers of tissue inflammation. However, treatment with MBSAE alleviated all the deleterious effects of alcohol consumption.ConclusionsMBSAE possess active compounds, which exert marked protective effects in chronic alcohol intoxication, possibly by regulating the erythrocytes osmotic stability as well as antioxidant and inflammatory mediators.

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“…The protective efficacy of the synthesized compounds against 2,2′-azobisĲ2-amidinopropane) dihydrochloride (AAPH) (Sigma Aldrich, Bangalore, India)-induced DNA damage was studied using plasmid pRSET-A plasmid DNA (Invitrogen, Thermo Fisher Scientific, India). 19 Briefly, 350 ng of plasmid DNA was dissolved in 10 μL of TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH 8.0), and then, AAPH was added to a final concentration of 10 mM with or without samples followed by incubation for a period of 20 min at room temperature. The plasmid DNA without sample was used as a control, and gallic acid (GA) was used as a standard antioxidant.…”

Section: Protective Effect On the Oxidation Of Biomolecules In Vitromentioning

confidence: 99%

“…Bovine serum albumin (BSA) (Merck, Bangalore, India) was oxidized by an azo compound, AAPH, which upon decomposition with oxygen generated a peroxyl radical. 19 Briefly, BSA (5 μg) was dissolved in phosphate buffer (pH 7.3) and incubated for 2 h with or without AAPH (400 mM final concentration) in the presence or absence of samples. After incubation, the protein samples were subjected to SDS-PAGE electrophoresis (10%); then, gels were stained with 0.15% Coomassie Brilliant Blue R-250, and the amount of protein damage was quantified by measuring the density of each band.…”

Section: Protein Oxidationmentioning

confidence: 99%

Development of piperazine-1-carbothioamide chitosan silver nanoparticles (P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking validation

Karthik¹,

Manukumar

Sandeep³

et al. 2018

Med. Chem. Commun.

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Natural products are important leads in drug discovery. The search for effective plant-derived agents or their synthetic analogues has continued to be of interest to biologists and chemists for a long time. Herein, we have synthesized a novel compound, P1C, and P1C-Tit*CAgNPs from chitosan; P1C is a precursor and an anti-inflammatory candidate, which has been validated by molecular docking studies. The synthesized P1C-Tit*CAgNPs showed monodisperse, spherical, and cationic nature and antioxidant properties, protecting destabilization of the erythrocyte membrane by the azo compound 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH); the involvement of NPs as a protective agent for biomolecules, such as DNA and protein, followed by the treatment of NPs with AAPH was confirmed. The inhibition of cellular damage and leakage of cellular inflammatory agents was confirmed by AFM, SEM, TEM, SDS-PAGE, LDH, and PLA2 enzyme inhibition studies. The anti-inflammatory property of P1C was further validated by molecular docking studies and showed that, the P1C best pose aligned to PLA2 compared to standard drug. The significant anticancer property of P1C-Tit*CAgNPs was confirmed against MCF7, U373, and C6 cancer cell lines. Thus, the present study highlights the synthesized P1C in P1C-Tit*CAgNPs as a target PLA2-specific anti-inflammatory candidate, and further tuning of small and development-functionalized nanoparticles has a great future in medicine; hence, their clinical applications are warranted.

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LC–ESI-MS/MS analysis of total oligomeric flavonoid fraction of Cyperus rotundus and its antioxidant, macromolecule damage protective and antihemolytic effects

Cited by 37 publications

References 39 publications

Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand

Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand

Myrtle berries seeds aqueous extract abrogates chronic alcohol consumption-induced erythrocytes osmotic stability disturbance, haematological and biochemical toxicity

Development of piperazine-1-carbothioamide chitosan silver nanoparticles (P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking validation

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