LC‐ESI‐MS/MS Chemical Characterization, Antioxidant and Antidiabetic Properties of Propolis Extracted with Organic Solvents from Eastern Anatolia Region

Akman, Tuğrul Çağrı; Simsek, Samed; Kayır, Ömer; Akşit, Zeynep; Akşit, Hüseyin; Genç, Nusret

doi:10.1002/cbdv.202201189

Cited by 14 publications

(5 citation statements)

References 39 publications

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“…[20,27] Given the chemical composition greatly influence its bioactivities, and the present ultrafiltration process can retain its active components profile, so it is suitable for raw propolis pretreatment. [28,29] The membrane filtration dynamic model fitted with y = (ax…”

Section: A Mwco 30 Kda Membrane Provides Satisfied Results Following ...mentioning

confidence: 99%

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Liang,

Wu,

Chen

et al. 2024

Chemistry & Biodiversity

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Propolis is one functional supplement with hundreds of years of usage. However, it’s rarely consumed directly for its resinous property. Herein, a pre‐treated process which can remove the impurity while preserve its bioactivities is needed to maximise its therapeutic opportunities. In the present study, a membrane‐based ultrafiltration process was developed on a KM1812‐NF experimental instrument. Using Brazilian green propolis as testing material, all experimental steps and parameters were sequentially optimized. In addition, a mathematical model was developed to fit the process. As a result, the optimum solvent was 60% ethanol adjusted to pH 8‐9, while the optimum MWCO (molecular weight cut‐off) value of membrane was 30 KDa. The membrane filtration dynamic model fitted with the function y=(ax+b)/(1+cx+dx2). The resulting propolis ultrafiltrate from Brazilian green propolis, termed P30K, contains the similar profile of flavonoids and phenolic acids as raw propolis. Meanwhile, the ORAC (oxygen radical absorbance capacity) value of P30K is 11429.45±1557.58 µM TE/g and the IC50 value of inhibition of fluorescent AGEs (advanced glycation end products) formation is 0.064 mg/mL. Our work provides an innovative alternative process for extraction of active compounds from propolis and reveals P30K as an efficient therapeutic antioxidant.

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Section: A Mwco 30 Kda Membrane Provides Satisfied Results Following ...mentioning

confidence: 99%

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Liang,

Wu,

Chen

et al. 2024

Chemistry & Biodiversity

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“…The column oven was set to 30 °C, the solvent flow rate was 0.7 mL/min and the analysis lasted for 34 min. The following are the criteria for the MS/MS analysis: Temperatures of the capillary are 300 °C, the vaporizer is 350 °C, the sheat gas pressure is 30 Arb, the aux gas pressure is 13 Arb, the positive and negative polarities are 2500 V and 4 µA, respectively 31 , 32 .…”

Section: Methodsmentioning

confidence: 99%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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Although the antioxidant properties of Melissa officinalis extract (Mox) are widely known, little work has focused on its protective capacity against heavy metal stress. The primary objective of this study was to determine the potential of Mox to mitigate manganese (II) chloride (MnCI2)-induced cyto-genotoxicity using the Allium and comet assays. Physiological, genotoxic, biochemical and anatomical parameters as well as the phenolic composition of Mox were examined in Allium cepa (L.). Application of 1000 µM MnCl2 reduced the rooting percentage, root elongation, weight gain, mitotic index and levels of chlorophyll a and chlorophyll b pigments compared to the control group. However, it increased micronuclei formation, chromosomal abnormality frequencies, tail DNA percentage, proline amount, lipid peroxidation level and meristematic damage severity. The activities of superoxide dismutase and catalase also increased. Chromosomal aberrations induced by MnCl2 were fragment, sticky chromosome, vagrant chromosome, unequal distribution of chromatin and bridge. Application of 250 mg/L Mox and 500 mg/L Mox along with MnCl2 significantly alleviated adverse effects dose dependently. The antioxidant activity bestowed by the phenolic compounds in Mox assisted the organism to combat MnCl2 toxicity. Consequently, Mox exerted remarkable protection against MnCl2 toxicity and it needs to be investigated further as a potential therapeutic option.

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“…Octadecylsilane-Hypersil 4.6*250 mm column was used. The MS/MS analysis conditions are as follows: capillary temperature, 300 °C; vaporizer temperature, 350 °C; sheath gas pressure, 30 Arb; Aux gas pressure, 13 Arb; positive polarity, 2500 V; negative polarity, 2500 V; and discharge current, 4 μA (Akman et al 2023 ; Kayir et al 2023 ). Quantification was evaluated according to MS/MS.…”

Section: Determination Of Formaldehyde Levelsmentioning

confidence: 99%

Effect of formaldehyde exposure on phytochemical content and functional activity of Agaricus bisporus (Lge.) Sing.

Kutluer

2024

Environ Sci Pollut Res

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In this study, the effect of formaldehyde on phytochemical content and antioxidant activity of Agaricus bisporus was investigated. Synthetic compost based on wheat straw was prepared by fermentation and disinfection. After steam pasteurization, 5 g of A. bisporus mycelia were inoculated into 1 kg of compost. To determine the effects of formaldehyde, 2, 4, and 6% concentrations were added to the composts, while compost without formaldehyde was used for the control group. The harvesting period was set at 10 weeks. Total phenolic and flavonoid content, macro- and microelement profile, and phenolic content were analyzed in the harvested A. bisporus samples. Macro- and microelement content was determined by ICP-OES, and phenolic compound profile was determined by LC-MS/MS analysis. Formaldehyde levels in A. bisporus samples were determined by the acetylacetone spectrophotometry method. The antioxidant capacity of A. bisporus samples was determined by DPPH scavenging activity; antimutagenic effects of samples were determined by Allium test. Application of 2, 4, and 6% formaldehyde resulted in a 1.12-, 1.19-, and 2.07-fold reduction in total phenolic content, respectively. The total phenolic content was reduced between 34.4% and 71.8%. These changes were confirmed by LC-MS/MS analysis. Compounds such as protocatechuic acid, salicylic acid, ferulic acid, and 4-OH benzoic acid, which were detected in the control group, could not be detected in the samples treated with 6% formaldehyde, and it was found that the application of formaldehyde reduced the phenolic content. Similar changes were also observed in macro- and microelements, and significant changes in elemental contents were observed after formaldehyde application. While the presence of formaldehyde at a low level, which may be due to natural production, was detected in the control group, a residue of 11.41 ± 0.93 mg/kg was determined in the 6% FMD applied group. All these changes resulted in a decrease in the antioxidant activity of A. bisporus. The DPPH scavenging activity, which was determined in the range of 21.6–73.3% in the control samples, decreased to 12.3–56.7% in the samples treated with formaldehyde. These results indicate that the application of formaldehyde at different stages of A. bisporus cultivation leads to significant changes in the nutritional value and biological activity of A. bisporus.

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LC‐ESI‐MS/MS Chemical Characterization, Antioxidant and Antidiabetic Properties of Propolis Extracted with Organic Solvents from Eastern Anatolia Region

Cited by 14 publications

References 39 publications

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Effect of formaldehyde exposure on phytochemical content and functional activity of Agaricus bisporus (Lge.) Sing.

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